Caspase 14 (CASP14) (NM_012114) Human Tagged ORF Clone

SKU RC210146

CASP14 (Myc-DDK-tagged)-Human caspase 14, apoptosis-related cysteine peptidase (CASP14)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Caspase 14
• Synonyms: ARCI12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210146 representing NM_012114; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCAATCCGCGGTCTTTGGAAGAGGAGAAATATGATATGTCAGGTGCCCGCCTGGCCCTAATACTGT; GTGTCACCAAAGCCCGGGAAGGTTCCGAAGAAGACCTGGATGCTCTGGAACACATGTTTCGGCAGCTGAG; ATTCGAAAGCACCATGAAAAGAGACCCCACTGCCGAGCAATTCCAGGAAGAGCTGGAAAAATTCCAGCAG; GCCATCGATTCCCGGGAAGATCCCGTCAGTTGTGCCTTCGTGGTACTCATGGCTCACGGGAGGGAAGGCT; TCCTCAAGGGAGAAGATGGGGAGATGGTCAAGCTGGAGAATCTCTTCGAGGCCCTGAACAACAAGAACTG; CCAGGCCCTGCGAGCTAAGCCCAAGGTGTACATCATACAGGCCTGTCGAGGAGAACAAAGGGACCCCGGT; GAAACAGTAGGTGGAGATGAGATTGTGATGGTCATCAAAGACAGCCCACAAACCATCCCAACATACACAG; ATGCCTTGCACGTTTATTCCACGGTAGAGGGATACATCGCCTACCGACATGATCAGAAAGGCTCATGCTT; TATCCAGACCCTGGTGGATGTGTTCACGAAGAGGAAAGGACATATCTTGGAACTTCTGACAGAGGTGACC; CGGCGGATGGCAGAAGCAGAGCTGGTTCAAGAAGGAAAAGCAAGGAAAACGAACCCTGAAATCCAAAGCA; CCCTCCGGAAACGGCTGTATCTGCAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210146 representing NM_012114; Red=Cloning site Green=Tags(s); ; MSNPRSLEEEKYDMSGARLALILCVTKAREGSEEDLDALEHMFRQLRFESTMKRDPTAEQFQEELEKFQQ; AIDSREDPVSCAFVVLMAHGREGFLKGEDGEMVKLENLFEALNNKNCQALRAKPKVYIIQACRGEQRDPG; ETVGGDEIVMVIKDSPQTIPTYTDALHVYSTVEGYIAYRHDQKGSCFIQTLVDVFTKRKGHILELLTEVT; RRMAEAELVQEGKARKTNPEIQSTLRKRLYLQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012114
• ORF Size: 726 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012114.3
• RefSeq Size: 777 bp
• RefSeq ORF: 729 bp
• Locus ID: 23581
• UniProt ID: P31944
• Cytogenetics: 19p13.12
• Protein Families: Druggable Genome
• MW: 27.5 kDa
• Summary: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008]