CD3D (NM_000732) Human Tagged ORF Clone
SKU
RC210010
CD3D (Myc-DDK-tagged)-Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CD3D |
| Synonyms | CD3-DELTA; IMD19; T3D |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000732 |
| ORF Size | 513 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000732.6 |
| RefSeq Size | 771 bp |
| RefSeq ORF | 516 bp |
| Locus ID | 915 |
| UniProt ID | P04234, B0YIY4 |
| Cytogenetics | 11q23.3 |
| Domains | ITAM |
| Protein Families | Druggable Genome |
| Protein Pathways | Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway |
| MW | 18.9 kDa |
| Summary | The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. provided by RefSeq, Feb 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000732" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210010L1 | Lenti ORF clone of Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210010L2 | Lenti ORF clone of Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RC210010L3 | Lenti ORF clone of Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210010L4 | Lenti ORF clone of Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG210010 | CD3D (tGFP-tagged) - Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC119732 | CD3D (untagged)-Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
Membralin Assembles a MAN1B1-VCP Complex to Target Foreign Glycoproteins from the Endoplasmic Reticulum to Lysosomes for Degradation
Zhang, Lu, Li et al
Adv Sci (Weinh) (2025)
Zhang, Lu, Li et al
Adv Sci (Weinh) (2025)
Cited by 7
AI Summary: OriGene products, specifically the pCMV6-CD3D-Myc-FLAG, pCMV6-NCSTN-Myc-FLAG, and pCMV6-VCP-Myc-FLAG plasmids (Cat# RC202082, RC218041, RC219485, RC210010, RC212646, RC211130), were used in this study to express and analyze key proteins involved in the Membralin-MAN1B1-VCP complex that mediates selective degradation of foreign glycoproteins in the endoplasmic reticulum.
Membralin Selects Foreign Glycoproteins from the Endoplasmic Reticulum to Lysosomes for Degradation
Zheng, Zhang, Lu et al
Research Square (2025)
Zheng, Zhang, Lu et al
Research Square (2025)
Cited by 1
AI Summary: OriGene products, including pCMV6-SERPINA1-Myc-Flag, pCMV6-COL2A1-Myc-Flag, pCMV6-DYSF-Myc-Flag, pCMV6-CD3D-Myc-Flag, pCMV6-NCSTN-Myc-Flag, and pCMV6-VCP-Myc-FLAG, were used as expression plasmids to study the roles of various proteins in Membralin-mediated degradation of foreign glycoproteins in the endoplasmic reticulum.
Snippet: pCDNA3.1-NHK was provided by Richard Sifers. pCMV6-SERPINA1-Myc-Flag, pCMV6-COL2A1-Myc-Flag, pCMV6-DYSF-Myc-Flag, pCMV6-CD3D-Myc-Flag, pCMV6-NCSTN-Myc-Flag, and pCMV6-VCP-Myc-FLAG were ordered from Origene (Cat# RC202082, RC218041, RC219485, RC210010, RC212646, RC211130). SERPINA1/AAT, NPC1, COL2A1, and DYSF mutants and pCMV6-VCP-His were created by PCR and ASiSI/MluI digestion followed by homol
A novel method to generate T-cell receptor-deficient chimeric antigen receptor T cells
Kamiya, Wong, Png et al
Blood Adv (2018) 2 (5), 517-528 DOI: 10.1182/bloodadvances.2017012823
Kamiya, Wong, Png et al
Blood Adv (2018) 2 (5), 517-528 DOI: 10.1182/bloodadvances.2017012823
AI Summary: OriGene’s CD3 subunit cDNA products were used to determine the specific CD3 subunit recognized by the antibody in the study, by subcloning and expressing them in K562 cells for subsequent binding analysis.
Snippet: To identify the CD3 subunit bound to the antibody derived from the PLU4 hybridoma, the cDNA of each CD3 subunit (Origene, Rockville, MD) was subcloned into MSCV-internal ribosome entry site-GFP and transduced into K562 cells. K562 cells were then permeabilized with 8E reagent (a permeabilization reagent developed in our laboratory), incubated with supernatant from PLU4 hybridoma cells, followed by...
Showing 1-3 of 3 papers.
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