RAB1B (NM_030981) Human Tagged ORF Clone
SKU
RC209927
RAB1B (Myc-DDK-tagged)-Human RAB1B, member RAS oncogene family (RAB1B)
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TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Target Symbol | RAB1B |
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Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC209927 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCCCGAATATGACTACCTGTTTAAGCTGCTTTTGATTGGCGACTCAGGCGTGGGCAAGTCATGCC TGCTCCTGCGGTTTGCTGATGACACGTACACAGAGAGCTACATCAGCACCATCGGGGTGGACTTCAAGAT CCGAACCATCGAGCTGGATGGCAAAACTATCAAACTTCAGATCTGGGACACAGCGGGCCAGGAACGGTTC CGGACCATCACTTCCAGCTACTACCGGGGGGCTCATGGCATCATCGTGGTGTATGACGTCACTGACCAGG AATCCTACGCCAACGTGAAGCAGTGGCTGCAGGAGATTGACCGCTATGCCAGCGAGAACGTCAATAAGCT CCTGGTGGGCAACAAGAGCGACCTCACCACCAAGAAGGTGGTGGACAACACCACAGCCAAGGAGTTTGCA GACTCTCTGGGCATCCCCTTCTTGGAGACGAGCGCCAAGAATGCCACCAATGTCGAGCAGGCGTTCATGA CCATGGCTGCTGAAATCAAAAAGCGGATGGGGCCTGGAGCAGCCTCTGGGGGCGAGCGGCCCAATCTCAA GATCGACAGCACCCCTGTAAAGCCGGCTGGCGGTGGCTGTTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209927 protein sequence
Red=Cloning site Green=Tags(s) MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERF RTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFA DSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_030981 |
ORF Size | 603 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_030981.3 |
RefSeq Size | 1955 bp |
RefSeq ORF | 606 bp |
Locus ID | 81876 |
UniProt ID | Q9H0U4 |
Cytogenetics | 11q13.2 |
Domains | ARF, RAB, RAN, ras, RAS, RHO |
Protein Families | Transcription Factors |
MW | 22.2 kDa |
Summary | Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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RC209927L1 | Lenti ORF clone of Human RAB1B, member RAS oncogene family (RAB1B), Myc-DDK-tagged | 10 ug |
$600.00
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RC209927L2 | Lenti ORF clone of Human RAB1B, member RAS oncogene family (RAB1B), mGFP tagged | 10 ug |
$600.00
|
|
RC209927L3 | Lenti ORF clone of Human RAB1B, member RAS oncogene family (RAB1B), Myc-DDK-tagged | 10 ug |
$600.00
|
|
RC209927L4 | Lenti ORF clone of Human RAB1B, member RAS oncogene family (RAB1B), mGFP tagged | 10 ug |
$600.00
|
|
RG209927 | RAB1B (tGFP-tagged) - Human RAB1B, member RAS oncogene family (RAB1B) | 10 ug |
$489.00
MSRP
$500.00
MSRP
$500.00
|
|
SC108613 | RAB1B (untagged)-Human RAB1B, member RAS oncogene family (RAB1B) | 10 ug |
$300.00
|
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.