LRRC25 (NM_145256) Human Tagged ORF Clone

SKU RC209911

LRRC25 (Myc-DDK-tagged)-Human leucine rich repeat containing 25 (LRRC25)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: LRRC25
• Synonyms: MAPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209911 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGGGGCACCCTGGCATGGACGCTGCTGTTGCCGCTGCTGCTGCGGGAGTCAGACAGCCTAGAACCGT; CGTGCACCGTGTCCTCCGCGGATGTGGACTGGAACGCGGAGTTCAGTGCCACGTGCCTGAATTTCAGTGG; CCTCAGCCTGAGCCTGCCTCACAACCAGTCTCTGCGGGCCAGCAACGTGATTCTCCTTGACCTGTCTGGG; AACGGCCTGCGAGAGCTTCCAGTGACCTTCTTTGCCCACCTGCAGAAGCTTGAGGTCCTGAACGTGCTAC; GCAACCCCTTGTCTCGTGTGGATGGGGCGCTGGCCGCCCGCTGTGACCTTGACCTGCAGGCCGACTGCAA; CTGTGCCCTGGAGTCCTGGCACGACATCCGCCGAGACAACTGCTCTGGCCAGAAGCCTCTGCTCTGCTGG; GACACAACCAGCTCCCAGCACAACCTCTCTGCCTTCCTGGAGGTCAGCTGCGCCCCTGGCCTGGCCTCTG; CAACTATCGGGGCAGTGGTGGTCAGCGGGTGCCTGCTTCTTGGACTTGCCATCGCTGGCCCTGTGCTGGC; CTGGAGACTCTGGCGATGCCGAGTGGCCAGAAGCCGGGAGCTGAACAAACCCTGGGCTGCTCAGGATGGG; CCCAAGCCCGGTTTAGGCTTGCAGCCACGGTACGGCAGCCGGAGCGCCCCCAAGCCCCAAGTGGCCGTGC; CATCCTGCCCCTCCACTCCCGACTATGAGAACATGTTTGTGGGCCAGCCAGCAGCCGAGCACCAGTGGGA; TGAACAAGGGGCTCACCCTTCAGAGGACAATGACTTTTACATCAACTACAAGGACATCGACCTGGCTTCC; CAGCCTGTCTACTGTAACCTGCAGTCACTGGGCCAGGCCTCAATGGATGAAGAGGAGTACGTGATCCCCG; GGCAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209911 protein sequence; Red=Cloning site Green=Tags(s); ; MGGTLAWTLLLPLLLRESDSLEPSCTVSSADVDWNAEFSATCLNFSGLSLSLPHNQSLRASNVILLDLSG; NGLRELPVTFFAHLQKLEVLNVLRNPLSRVDGALAARCDLDLQADCNCALESWHDIRRDNCSGQKPLLCW; DTTSSQHNLSAFLEVSCAPGLASATIGAVVVSGCLLLGLAIAGPVLAWRLWRCRVARSRELNKPWAAQDG; PKPGLGLQPRYGSRSAPKPQVAVPSCPSTPDYENMFVGQPAAEHQWDEQGAHPSEDNDFYINYKDIDLAS; QPVYCNLQSLGQASMDEEEYVIPGH; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145256
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145256.2, NP_660299.2
• RefSeq Size: 2430 bp
• RefSeq ORF: 918 bp
• Locus ID: 126364
• UniProt ID: Q8N386
• Cytogenetics: 19p13.11
• Domains: LRR
• Protein Families: Transmembrane
• MW: 33.2 kDa
• Summary: Plays a role in the inhibition of RLR-mediated type I interferon signaling pathway by targeting DDX58/RIG-I for autophagic degradation. Interacts specifically with ISG15-associated DDX58 to promote interaction between DDX58 and the autophagic cargo receptor p62/SQSTM1 to mediate DDX58 degradation via selective autophagy (PubMed:29288164). Plays also a role in the inhibition of NF-kappa-B signaling pathway and inflammatory response by promoting the degradation of p65/RELA.[UniProtKB/Swiss-Prot Function]