NECAB1 (NM_022351) Human Tagged ORF Clone

SKU
RC209704
NECAB1 (Myc-DDK-tagged)-Human N-terminal EF-hand calcium binding protein 1 (NECAB1)
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$457.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NECAB1
Synonyms EFCBP1; STIP-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209704 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGATTCCCAGGAGACATCGCCGTCCTCCAACAACTCCTCGGAGGAGCTCAGCTCTGCTCTGCACC
TGTCCAAGGGCATGTCGATCTTCCTCGACATACTGAGGAGAGCAGACAAAAATGATGATGGAAAATTATC
CTTTGAAGAATTCAAAGCATATTTTGCAGATGGTGTTCTCAGTGGAGAAGAATTACACGAGCTTTTCCAT
ACCATTGATACACATAATACTAATAATCTTGACACAGAAGAGCTATGTGAATATTTTTCTCAGCACTTGG
GCGAGTATGAGAATGTACTAGCAGCACTTGAAGGCCTGAATCTTTCCATCCTGAAGGCAATGGGCAAAAC
AAAGAAAGACTACCAAGAAGCCTCCAATTTGGAACAATTCGTAACTAGATTTTTATTGAAGGAAACCCTG
AATCAGCTGCAGTCTCTCCAGAATTCCCTGGAATGTGCCATGGAAACTACTGAGGAGCAAACCCGTCAAG
AAAGGCAAGGGCCAGCCAAGCCAGAAGTCCTGTCGATTCAATGGCCTGGAAAACGATCAAGCCGCCGAGT
CCAGAGACACAACAGCTTCTCCCCAAACAGCCCTCAGTTTAATGTCAGCGGTCCAGGCTTATTAGAAGAA
GACAACCAGTGGATGACCCAGATAAATAGACTCCAGAAATTAATTGATAGACTGGAAAAGAAGGATCTCA
AACTCGAACCACCAGAAGAAGAAATTATTGAAGGGAATACTAAATCTCACATCATGCTTGTGCAGCGGCA
GATGTCTGTGATAGAAGAGGACCTGGAAGAATTCCAGCTCGCTCTGAAACACTACGTGGAGAGTGCTTCC
TCTCAAAGTGGATGCTTGCGTATTTCTATACAGAAGCTTTCAAATGAATCTCGCTACATGATCTATGAGT
TCTGGGAGAATAGTAGTGTATGGAATAGCCACCTTCAGACAAATTATAGCAAGACATTCCAAAGAAGTAA
TGTGGATTTCTTGGAAACTCCAGAACTCACATCTACAATGCTAGTTCCTGCTTCGTGGTGGATCCTGAAC
AAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209704 protein sequence
Red=Cloning site Green=Tags(s)

MEDSQETSPSSNNSSEELSSALHLSKGMSIFLDILRRADKNDDGKLSFEEFKAYFADGVLSGEELHELFH
TIDTHNTNNLDTEELCEYFSQHLGEYENVLAALEGLNLSILKAMGKTKKDYQEASNLEQFVTRFLLKETL
NQLQSLQNSLECAMETTEEQTRQERQGPAKPEVLSIQWPGKRSSRRVQRHNSFSPNSPQFNVSGPGLLEE
DNQWMTQINRLQKLIDRLEKKDLKLEPPEEEIIEGNTKSHIMLVQRQMSVIEEDLEEFQLALKHYVESAS
SQSGCLRISIQKLSNESRYMIYEFWENSSVWNSHLQTNYSKTFQRSNVDFLETPELTSTMLVPASWWILN
N

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_022351
ORF Size 1053 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022351.5
RefSeq Size 5140 bp
RefSeq ORF 1056 bp
Locus ID 64168
UniProt ID Q8N987
Cytogenetics 8q21.3
Domains ABM, EFh
MW 40.5 kDa
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SKU Description Size Price
RC209704L1 Lenti ORF clone of Human N-terminal EF-hand calcium binding protein 1 (NECAB1), Myc-DDK-tagged 10 ug
$757.00
RC209704L2 Lenti ORF clone of Human N-terminal EF-hand calcium binding protein 1 (NECAB1), mGFP tagged 10 ug
$757.00
RC209704L3 Lenti ORF clone of Human N-terminal EF-hand calcium binding protein 1 (NECAB1), Myc-DDK-tagged 10 ug
$757.00
RC209704L4 Lenti ORF clone of Human N-terminal EF-hand calcium binding protein 1 (NECAB1), mGFP tagged 10 ug
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RG209704 NECAB1 (tGFP-tagged) - Human N-terminal EF-hand calcium binding protein 1 (NECAB1) 10 ug
$489.00 MSRP $657.00 MSRP $657.00
SC108309 NECAB1 (untagged)-Human N-terminal EF-hand calcium binding protein 1 (NECAB1) 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.