ZNF784 (NM_203374) Human Tagged ORF Clone

SKU
RC209589
ZNF784 (Myc-DDK-tagged)-Human zinc finger protein 784 (ZNF784)
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF784
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209589 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCTGCGCGCCCAGAGGCCCAGAGTCGGAGCTCACCGACTCCGGAGTCGCGATCCCAGGAGCCAC
TGGACCTGGTCCTGGTGCCTGATGACTGCCGGCCTGGCACACCCCCGAGTGACCTCATCGAGATCCAGGT
GGTGAAGGTGACGGACACCACGCTGGTCCCTGAGCCCCCGGAGCCAGGTTCTTTCCACTGTGCCTTGTGC
CCTGCTGCCTTCCGGCTGGTTTCCGAGCTGCTGTTCCACGAACATGGCCACTTGGCTGGGGCCGAGGGAG
GCGGGCAGGGTGGGGACCCGAGCCGGTGCCACGTGTGCGGCCACAGCTGCCCGGGCCCCGCCAGCCTGCG
CGCGCACTACAGCTTGCACACGGGGGAGCGGCCCTACCGCTGCGCGCTCTGCCCCCGCGCCTTCAAGGCC
TTGGCGCCCCTGCTCCGGCACCAGCACCGGCACGGGGTGGAGCCGGGCACCTCTCGGAGGCCTCCGGACA
CAGCGGCCGTTGCAGAACAGAGGCCCGGGGTGGCCCCGGAGAGGGCGGAGGTGGTGATGGCGGCGGCGGC
GGCGGGCGCAGCGGTGGGGAAGCCTTTTGCCTGCAGGTTCTGCGCCAAGCCCTTCCGCCGCTCCTCAGAC
ATGCGAGACCACGAGCGCGTGCACACTGGCGAGCGGCCATACCATTGCGGCATCTGCGGCAAGGGCTTCA
CCCAGTCCTCGGTGCTTAGCGGCCACGCCCGCATCCACACTGGCGAGCGCCCGTTCCGCTGCACGCTCTG
CGACCGCACCTTCAACAACTCCTCCAACTTCCGCAAGCACCAGCGCACCCACTTCCACGGGCCGGGGCCG
GGGCTGGGAGACTCTGGAGGCCAGCTGGGCTCGTCGGCGGCTGAGGGGTCGGGGAGCGGGTGTGGGGTAG
GGGACCCTGCGGAGGAGGGGCGGGGGGAGACCGCGAAGGTGAAGGTGGAGGCCGACCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209589 protein sequence
Red=Cloning site Green=Tags(s)

MAAARPEAQSRSSPTPESRSQEPLDLVLVPDDCRPGTPPSDLIEIQVVKVTDTTLVPEPPEPGSFHCALC
PAAFRLVSELLFHEHGHLAGAEGGGQGGDPSRCHVCGHSCPGPASLRAHYSLHTGERPYRCALCPRAFKA
LAPLLRHQHRHGVEPGTSRRPPDTAAVAEQRPGVAPERAEVVMAAAAAGAAVGKPFACRFCAKPFRRSSD
MRDHERVHTGERPYHCGICGKGFTQSSVLSGHARIHTGERPFRCTLCDRTFNNSSNFRKHQRTHFHGPGP
GLGDSGGQLGSSAAEGSGSGCGVGDPAEEGRGETAKVKVEADQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_203374
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_203374.2
RefSeq Size 2006 bp
RefSeq ORF 972 bp
Locus ID 147808
UniProt ID Q8NCA9
Cytogenetics 19q13.42
MW 34.2 kDa
Summary May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]
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Citations

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