Collagen IX (COL9A1) (NM_078485) Human Tagged ORF Clone

SKU RC209473

COL9A1 (Myc-DDK-tagged)-Human collagen, type IX, alpha 1 (COL9A1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Collagen IX
• Synonyms: DJ149L1.1.2; EDM6; MED; STL4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209473 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCTGGACTGCGCGGGACCGCGGGGCCCTGGGGCTGCTGCTGTTGGGGCTCTGCTTGTGCGCGGCTC; AAAGAGGTCCCCCGGGTGAGCAGGGTCCTCCCGGGCCTCCGGGCCCCCCTGGAGTTCCAGGCATCGATGG; CATCGACGGTGACCGAGGTCCTAAGGGCCCCCCGGGCCCCCCGGGTCCTGCAGGTGAACCGGGAAAGCCA; GGAGCTCCAGGCAAGCCTGGCACACCTGGCGCTGATGGATTAACAGGACCTGATGGATCCCCTGGCTCCA; TTGGGTCAAAGGGACAAAAAGGAGAACCTGGTGTGCCTGGATCGCGTGGATTTCCAGGCCGTGGTATTCC; TGGACCCCCTGGTCCTCCTGGGACAGCAGGACTCCCTGGAGAGCTTGGCCGTGTAGGACCTGTTGGTGAC; CCTGGGAGAAGAGGACCACCTGGCCCCCCTGGCCCCCCAGGACCCAGAGGAACAATTGGCTTTCATGATG; GAGATCCATTGTGTCCCAATGCCTGTCCACCAGGTCGCTCAGGATATCCAGGCCTACCAGGCATGAGGGG; TCATAAAGGGGCTAAAGGAGAAATTGGTGAACCAGGAAGACAAGGACACAAGGGTGAAGAAGGTGACCAG; GGAGAACTCGGAGAAGTTGGAGCTCAAGGACCTCCAGGAGCCCAGGGTTTGCGAGGCATCACCGGCATAG; TTGGGGACAAAGGGGAAAAAGGTGCTCGGGGCTTAGATGGTGAACCTGGGCCTCAGGGTCTTCCTGGTGC; ACCTGGTGATCAAGGACAGCGAGGACCTCCAGGAGAAGCAGGTCCCAAAGGAGATAGAGGGGCTGAAGGT; GCTAGAGGAATTCCTGGTCTCCCTGGGCCCAAAGGAGACACGGGTTTGCCAGGTGTGGATGGCCGTGATG; GGATCCCTGGAATGCCTGGAACAAAGGGTGAACCAGGAAAACCTGGGCCTCCTGGTGATGCAGGATTGCA; GGGGTTACCAGGTGTACCGGGAATTCCTGGTGCAAAGGGTGTTGCTGGTGAAAAGGGTAGCACAGGTGCT; CCAGGGAAGCCTGGTCAGATGGGAAATTCAGGCAAACCGGGCCAACAGGGGCCTCCAGGAGAGGTGGGAC; CCCGAGGACCCCAGGGGCTTCCTGGCAGTAGAGGAGAATTAGGACCAGTGGGATCCCCAGGCCTACCAGG; TAAACTGGGTTCTCTGGGTAGCCCTGGCCTCCCTGGCTTGCCTGGGCCCCCTGGACTTCCTGGAATGAAA; GGTGACAGGGGTGTAGTCGGTGAACCGGGTCCAAAGGGTGAACAGGGTGCCTCTGGTGAAGAAGGTGAAG; CAGGAGAAAGGGGGGAACTTGGAGATATAGGATTACCTGGCCCAAAGGGATCTGCAGGTAATCCTGGGGA; ACCTGGCTTGAGAGGGCCTGAGGGAAGTCGGGGGCTTCCTGGAGTGGAAGGACCAAGAGGACCACCTGGA; CCCCGGGGTGTGCAGGGAGAACAGGGTGCCACCGGCCTGCCTGGTGTCCAGGGCCCTCCGGGTAGAGCAC; CGACAGATCAGCACATTAAGCAGGTTTGCATGAGAGTCATACAAGAACATTTTGCTGAGATGGCTGCCAG; TCTTAAGCGTCCAGACTCAGGTGCCACTGGGCTTCCTGGAAGGCCTGGCCCTCCTGGTCCCCCCGGCCCT; CCTGGAGAGAATGGTTTCCCAGGCCAGATGGGAATTCGTGGCCTTCCGGGCATTAAGGGGCCCCCTGGTG; CTCTTGGTTTGAGGGGACCTAAAGGTGACTTGGGAGAAAAGGGGGAGCGTGGCCCTCCAGGAAGAGGTCC; CAACGGTTTGCCTGGAGCTATAGGTCTCCCAGGTGACCCAGGCCCTGCCAGCTATGGCAGAAATGGCCGA; GACGGTGAGCGAGGCCCCCCAGGGGTGGCAGGAATTCCTGGAGTGCCTGGACCCCCGGGACCTCCTGGGC; TTCCCGGTTTCTGTGAGCCAGCCTCCTGCACCATGCAGGCTGGTCAGCGAGCATTTAACAAAGGGCCTGA; CCCT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209473 protein sequence; Red=Cloning site Green=Tags(s); ; MAWTARDRGALGLLLLGLCLCAAQRGPPGEQGPPGPPGPPGVPGIDGIDGDRGPKGPPGPPGPAGEPGKP; GAPGKPGTPGADGLTGPDGSPGSIGSKGQKGEPGVPGSRGFPGRGIPGPPGPPGTAGLPGELGRVGPVGD; PGRRGPPGPPGPPGPRGTIGFHDGDPLCPNACPPGRSGYPGLPGMRGHKGAKGEIGEPGRQGHKGEEGDQ; GELGEVGAQGPPGAQGLRGITGIVGDKGEKGARGLDGEPGPQGLPGAPGDQGQRGPPGEAGPKGDRGAEG; ARGIPGLPGPKGDTGLPGVDGRDGIPGMPGTKGEPGKPGPPGDAGLQGLPGVPGIPGAKGVAGEKGSTGA; PGKPGQMGNSGKPGQQGPPGEVGPRGPQGLPGSRGELGPVGSPGLPGKLGSLGSPGLPGLPGPPGLPGMK; GDRGVVGEPGPKGEQGASGEEGEAGERGELGDIGLPGPKGSAGNPGEPGLRGPEGSRGLPGVEGPRGPPG; PRGVQGEQGATGLPGVQGPPGRAPTDQHIKQVCMRVIQEHFAEMAASLKRPDSGATGLPGRPGPPGPPGP; PGENGFPGQMGIRGLPGIKGPPGALGLRGPKGDLGEKGERGPPGRGPNGLPGAIGLPGDPGPASYGRNGR; DGERGPPGVAGIPGVPGPPGPPGLPGFCEPASCTMQAGQRAFNKGPDP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_078485
• ORF Size: 2034 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_078485.4
• RefSeq Size: 3073 bp
• RefSeq ORF: 2037 bp
• Locus ID: 1297
• UniProt ID: P20849
• Cytogenetics: 6q13
• Domains: Collagen
• Protein Families: Druggable Genome
• MW: 64.4 kDa
• Summary: This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]