CRSP8 (MED27) (NM_004269) Human Tagged ORF Clone

SKU RC209398

MED27 (Myc-DDK-tagged)-Human mediator complex subunit 27 (MED27)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CRSP8
• Synonyms: CRAP34; CRSP8; CRSP34; MED3; NEDSCAC; TRAP37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209398 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGACGTGATAAATGTCAGTGTGAACCTGGAGGCCTTTTCCCAGGCCATTAGTGCCATCCAGGCGC; TGCGCTCCAGCGTGAGCAGGGTGTTCGACTGCCTGAAGGATGGGATGCGGAACAAGGAGACGCTGGAGGG; CCGGGAGAAGGCCTTTATTGCGCACTTCCAGGACAACTTACATTCGGTCAACCGGGACCTCAATGAGCTG; GAACGTCTGAGCAATCTGGTAGGCAAGCCATCTGAGAACCATCCTCTTCATAACAGTGGGCTGTTAAGCC; TGGATCCTGTGCAGGACAAAACTCCTCTCTATAGTCAACTCCTTCAAGCATATAAGTGGTCAAACAAGTT; GCAGTACCATGCAGGACTAGCATCTGGCCTTTTAAATCAGCAGTCATTGAAGCGTTCCGCTAATCAGATG; GGAGTATCTGCCAAACGTAGACCAAAGGCTCAGCCCACAACTCTTGTCCTACCACCTCAATATGTTGATG; ATGTGATCAGCCGCATTGACAGGATGTTTCCTGAAATGTCCATCCACTTATCCAGACCCAATGGAACATC; AGCAATGCTTCTGGTGACCTTGGGAAAGGTGTTGAAAGTGATCGTCGTCATGCGGAGCCTGTTCATTGAT; CGAACAATAGTAAAGGGATATAACGAGAATGTCTACACAGAAGATGGCAAGCTTGATATATGGTCCAAAT; CCAACTATCAAGTATTCCAGAAGGTGACAGACCATGCCACCACTGCCCTGCTCCACTATCAGCTGCCCCA; GATGCCGGATGTCGTGGTCCGATCCTTCATGACCTGGTTAAGAAGTTACATAAAGCTGTTCCAGGCCCCG; TGCCAGCGCTGCGGGAAGTTTCTGCAGGACGGCCTTCCCCCGACATGGAGGGATTTCCGAACCCTCGAAG; CCTTCCATGACACCTGCCGGCAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209398 protein sequence; Red=Cloning site Green=Tags(s); ; MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNEL; ERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQM; GVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFID; RTIVKGYNENVYTEDGKLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAP; CQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004269
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004269.4
• RefSeq Size: 1418 bp
• RefSeq ORF: 936 bp
• Locus ID: 9442
• UniProt ID: Q6P2C8
• Cytogenetics: 9q34.13
• MW: 35.4 kDa
• Summary: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]