FATP1 (SLC27A1) (NM_198580) Human Tagged ORF Clone

SKU RC209285

SLC27A1 (Myc-DDK-tagged)-Human solute carrier family 27 (fatty acid transporter), member 1 (SLC27A1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FATP1
• Synonyms: ACSVL5; FATP; FATP-1; FATP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209285 representing NM_198580; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCGGGCTCCGGGTGCGGGCGCGGCCTCGGTGGTCTCGCTGGCGCTGTTGTGGCTGCTGGGGCTGCCGT; GGACCTGGAGCGCGGCAGCGGCGCTCGGCGTGTACGTGGGCAGCGGCGGCTGGCGCTTCCTGCGCATCGT; CTGCAAGACCGCGAGGCGAGACCTCTTCGGTCTCTCTGTGCTGATCCGCGTGCGCCTGGAGCTGCGGCGG; CACCAGCGTGCCGGCCACACCATCCCGCGCATCTTTCAGGCGGTAGTGCAGCGACAGCCCGAGCGCCTGG; CGCTGGTGGATGCCGGGACCGGCGAGTGCTGGACCTTTGCGCAGCTGGACGCCTACTCCAATGCGGTAGC; CAACCTCTTCCGCCAGCTGGGCTTCGCGCCGGGCGACGTGGTGGCCATCTTCCTGGAGGGCCGGCCGGAG; TTCGTGGGGCTGTGGCTGGGCCTGGCCAAGGCGGGCATGGAGGCCGCGCTGCTCAACGTGAACCTGCGGC; GCGAGCCCCTGGCCTTCTGCCTGGGCACCTCGGGCGCTAAGGCCCTGATCTTTGGAGGAGAAATGGTGGC; GGCGGTGGCCGAAGTGAGCGGGCATCTGGGGAAAAGTTTGATCAAGTTCTGCTCTGGAGACTTGGGGCCC; GAGGGCATCTTGCCGGACACCCACCTCCTGGACCCGCTGCTGAAGGAGGCCTCTACTGCCCCCTTGGCAC; AGATCCCCAGCAAGGGCATGGACGATCGTCTTTTCTACATCTACACGTCGGGGACCACCGGGCTGCCCAA; GGCTGCCATTGTCGTGCACAGCAGGTACTACCGCATGGCAGCCTTCGGCCACCACGCCTACCGCATGCAG; GCGGCTGACGTGCTCTATGACTGCCTGCCCCTGTACCACTCGGCAGGAAACATCATCGGCGTGGGGCAGT; GTCTCATCTATGGGCTGACAGTCGTCCTCCGCAAGAAATTCTCGGCCAGCCGCTTCTGGGACGACTGCAT; CAAGTACAACTGCACGGTGGTTCAGTACATCGGGGAGATCTGCCGCTACCTGCTGAAGCAGCCGGTGCGC; GAGGCGGAGAGGCGACACCGCGTGCGCCTGGCGGTGGGGAACGGGCTGCGTCCTGCCATCTGGGAGGAGT; TCACGGAGCGCTTCGGCGTACGCCAAATCGGGGAGTTCTACGGCGCCACCGAGTGCAACTGCAGCATTGC; CAACATGGACGGCAAGGTCGGCTCCTGTGGTTTCAACAGCCGCATCCTGCCCCACGTGTACCCCATCCGG; CTGGTGAAGGTCAATGAGGACACAATGGAGCTGCTGCGGGATGCCCAGGGCCTCTGCATCCCCTGCCAGG; CCGGGGAGCCTGGCCTCCTTGTGGGTCAGATCAACCAACAGGACCCGCTGCGCCGCTTCGATGGCTATGT; CAGCGAGAGCGCCACCAGCAAGAAGATCGCCCACAGCGTCTTCAGCAAGGGCGACAGCGCCTACCTCTCA; GGTGACGTGCTAGTGATGGATGAGCTGGGCTACATGTACTTCCGGGACCGTAGCGGGGACACCTTCCGCT; GGCGAGGGGAGAACGTCTCCACCACCGAGGTGGAGGGCGTGCTGAGCCGCCTGCTGGGCCAGACAGACGT; GGCCGTCTATGGGGTGGCTGTTCCAGGAGTGGAGGGTAAGGCAGGGATGGCGGCCGTCGCAGACCCCCAC; AGCCTGCTGGACCCCAACGCGATATACCAGGAGCTGCAGAAGGTGCTGGCACCCTATGCCCGGCCCATCT; TCCTGCGCCTCCTGCCCCAGGTGGACACCACAGGCACCTTCAAGATCCAGAAGACGAGGCTGCAGCGAGA; GGGCTTTGACCCACGCCAGACCTCAGACCGGCTCTTCTTCCTGGACCTGAAGCAGGGCCACTACCTGCCC; TTAAATGAGGCAGTCTACACTCGCATCTGCTCGGGCGCCTTCGCCCTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209285 representing NM_198580; Red=Cloning site Green=Tags(s); ; MRAPGAGAASVVSLALLWLLGLPWTWSAAAALGVYVGSGGWRFLRIVCKTARRDLFGLSVLIRVRLELRR; HQRAGHTIPRIFQAVVQRQPERLALVDAGTGECWTFAQLDAYSNAVANLFRQLGFAPGDVVAIFLEGRPE; FVGLWLGLAKAGMEAALLNVNLRREPLAFCLGTSGAKALIFGGEMVAAVAEVSGHLGKSLIKFCSGDLGP; EGILPDTHLLDPLLKEASTAPLAQIPSKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRMAAFGHHAYRMQ; AADVLYDCLPLYHSAGNIIGVGQCLIYGLTVVLRKKFSASRFWDDCIKYNCTVVQYIGEICRYLLKQPVR; EAERRHRVRLAVGNGLRPAIWEEFTERFGVRQIGEFYGATECNCSIANMDGKVGSCGFNSRILPHVYPIR; LVKVNEDTMELLRDAQGLCIPCQAGEPGLLVGQINQQDPLRRFDGYVSESATSKKIAHSVFSKGDSAYLS; GDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEGVLSRLLGQTDVAVYGVAVPGVEGKAGMAAVADPH; SLLDPNAIYQELQKVLAPYARPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGHYLP; LNEAVYTRICSGAFAL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198580
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198580.3
• RefSeq Size: 3562 bp
• RefSeq ORF: 1941 bp
• Locus ID: 376497
• UniProt ID: Q6PCB7
• Cytogenetics: 19p13.11
• Protein Families: Transmembrane
• Protein Pathways: PPAR signaling pathway
• MW: 70.9 kDa
• Summary: Mediates the ATP-dependent import of long-chain fatty acids (LCFA) into the cell by mediating their translocation at the plasma membrane (PubMed:28178239). Has also an acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism (By similarity).[UniProtKB/Swiss-Prot Function]