Natriuretic Peptide Receptor A (NPR1) (NM_000906) Human Tagged ORF Clone

SKU RC209267

NPR1 (Myc-DDK-tagged)-Human natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) (NPR1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Natriuretic Peptide Receptor A
• Synonyms: ANPa; ANPRA; GUC2A; GUCY2A; NPRA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209267 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCGGGGCCCCGGCGCCCCGCTGGCTCCCGCCTGCGCCTGCTCCTGCTCCTGCTGCTGCCGCCGCTGC; TGCTGCTGCTCCGGGGCAGCCACGCGGGCAACCTGACGGTAGCCGTGGTACTGCCGCTGGCCAATACCTC; GTACCCCTGGTCGTGGGCGCGCGTGGGACCCGCCGTGGAGCTGGCCCTGGCCCAGGTGAAGGCGCGCCCC; GACTTGCTGCCGGGCTGGACGGTCCGCACGGTGCTGGGCAGCAGCGAAAACGCGCTGGGCGTCTGCTCCG; ACACCGCAGCGCCCCTGGCCGCGGTGGACCTCAAGTGGGAGCACAACCCCGCTGTGTTCCTGGGCCCCGG; CTGCGTGTACGCCGCCGCCCCAGTGGGGCGCTTCACCGCGCACTGGCGGGTCCCGCTGCTGACCGCCGGC; GCCCCGGCGCTGGGCTTCGGTGTCAAGGACGAGTATGCGCTGACCACCCGCGCGGGGCCCAGCTACGCCA; AGCTGGGGGACTTCGTGGCGGCGCTGCACCGACGGCTGGGCTGGGAGCGCCAAGCGCTCATGCTCTACGC; CTACCGGCCGGGTGACGAAGAGCACTGCTTCTTCCTCGTGGAGGGGCTGTTCATGCGGGTCCGCGACCGC; CTCAATATTACGGTGGACCACCTGGAGTTCGCCGAGGACGACCTCAGCCACTACACCAGGCTGCTGCGGA; CCATGCCGCGCAAAGGCCGAGTTATCTACATCTGCAGCTCCCCTGATGCCTTCAGAACCCTCATGCTCCT; GGCCCTGGAAGCTGGCTTGTGTGGGGAGGACTACGTTTTCTTCCACCTGGATATCTTTGGGCAAAGCCTG; CAAGGTGGACAGGGCCCTGCTCCCCGCAGGCCCTGGGAGAGAGGGGATGGGCAGGATGTCAGTGCCCGCC; AGGCCTTTCAGGCTGCCAAAATCATTACATATAAAGACCCAGATAATCCCGAGTACTTGGAATTCCTGAA; GCAGTTAAAACACCTGGCCTATGAGCAGTTCAACTTCACCATGGAGGATGTCCTGGTGAACACCATCCCA; GCATCCTTCCACGACGGGCTCCTGCTCTATATCCAGGCAGTGACGGAGACTCTGGCACATGGGGGAACTG; TTACTGATGGGGAGAACATCACTCAGCGGATGTGGAACCGAAGCTTTCAAGGTGTGACAGGATACCTGAA; AATTGATAGCAGTGGCGATCGGGAAACAGACTTCTCCCTCTGGGATATGGATCCCGAGAATGGTGCCTTC; AGGGTTGTACTGAACTACAATGGGACTTCCCAAGAGCTGGTGGCTGTGTCGGGGCGCAAACTGAACTGGC; CCCTGGGGTACCCTCCTCCTGACATCCCCAAATGTGGCTTTGACAACGAAGACCCAGCATGCAACCAAGA; TCACCTTTCCACCCTGGAGGTGCTGGCTTTGGTGGGCAGCCTCTCCTTGCTCGGCATTCTGATTGTCTCC; TTCTTCATATACAGGAAGATGCAGCTGGAGAAGGAACTGGCCTCGGAGCTGTGGCGGGTGCGCTGGGAGG; ACGTTGAGCCCAGTAGCCTTGAGAGGCACCTGCGGAGTGCAGGCAGCCGGCTGACCCTGAGCGGGAGAGG; CTCCAATTACGGCTCCCTGCTAACCACAGAGGGCCAGTTCCAAGTCTTTGCCAAGACAGCATATTATAAG; GGCAACCTCGTGGCTGTGAAACGTGTGAACCGTAAACGCATTGAGCTGACACGAAAAGTCCTGTTTGAAC; TGAAGCATATGCGGGATGTGCAGAATGAACACCTGACCAGGTTTGTGGGAGCCTGCACCGACCCCCCCAA; TATCTGCATCCTCACAGAGTACTGTCCCCGTGGGAGCCTGCAGGACATTCTGGAGAATGAGAGCATCACC; CTGGACTGGATGTTCCGGTACTCACTCACCAATGACATCGTCAAGGGCATGCTGTTTCTACACAATGGGG; CTATCTGTTCCCATGGGAACCTCAAGTCATCCAACTGCGTGGTAGATGGGCGCTTTGTGCTCAAGATCAC; CGACTATGGGCTGGAGAGCTTCAGGGACCTGGACCCAGAGCAAGGACACACCGTTTATGCCAAAAAGCTG; TGGACGGCCCCTGAGCTCCTGCGAATGGCTTCACCCCCTGTGCGGGGCTCCCAGGCTGGTGACGTATACA; GCTTTGGGATCATCCTTCAGGAGATTGCCCTGAGGAGTGGGGTCTTCCACGTGGAAGGTTTGGACCTGAG; CCCCAAAGAGATCATCGAGCGGGTGACTCGGGGTGAGCAGCCCCCCTTCCGGCCCTCCCTGGCCCTGCAG; AGTCACCTGGAGGAGTTGGGGCTGCTCATGCAGCGGTGCTGGGCTGAGGACCCACAGGAGAGGCCACCAT; TCCAGCAGATCCGCCTGACGTTGCGCAAATTTAACAGGGAGAACAGCAGCAACATCCTGGACAACCTGCT; GTCCCGCATGGAGCAGTACGCGAACAATCTGGAGGAACTGGTGGAGGAGCGGACCCAGGCATACCTGGAG; GAGAAGCGCAAGGCTGAGGCCCTGCTCTACCAGATCCTGCCTCACTCAGTGGCTGAGCAGCTGAAGCGTG; GGGAGACGGTGCAGGCCGAAGCCTTTGACAGTGTTACCATCTACTTCAGTGACATTGTGGGTTTCACAGC; GCTGTCGGCGGAGAGCACGCCCATGCAGGTGGTGACCCTGCTCAATGACCTGTACACTTGCTTTGATGCT; GTCATAGACAACTTTGATGTGTACAAGGTGGAGACAATTGGCGATGCCTACATGGTGGTGTCAGGGCTCC; CTGTGCGGAACGGGCGGCTACACGCCTGCGAGGTAGCCCGCATGGCCCTGGCACTGCTGGATGCTGTGCG; CTCCTTCCGAATCCGCCACCGGCCCCAGGAGCAGCTGCGCTTGCGCATTGGCATCCACACAGGACCTGTG; TGTGCTGGAGTGGTGGGACTGAAGATGCCCCGTTACTGTCTCTTTGGGGATACAGTCAACACAGCCTCAA; GAATGGAGTCTAATGGGGAAGCCCTGAAGATCCACTTGTCTTCTGAGACCAAGGCTGTCCTGGAGGAGTT; TGGTGGTTTCGAGCTGGAGCTTCGAGGGGATGTAGAAATGAAGGGCAAAGGCAAGGTTCGGACCTACTGG; CTCCTTGGGGAGAGGGGGAGTAGCACCCGAGGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209267 protein sequence; Red=Cloning site Green=Tags(s); ; MPGPRRPAGSRLRLLLLLLLPPLLLLLRGSHAGNLTVAVVLPLANTSYPWSWARVGPAVELALAQVKARP; DLLPGWTVRTVLGSSENALGVCSDTAAPLAAVDLKWEHNPAVFLGPGCVYAAAPVGRFTAHWRVPLLTAG; APALGFGVKDEYALTTRAGPSYAKLGDFVAALHRRLGWERQALMLYAYRPGDEEHCFFLVEGLFMRVRDR; LNITVDHLEFAEDDLSHYTRLLRTMPRKGRVIYICSSPDAFRTLMLLALEAGLCGEDYVFFHLDIFGQSL; QGGQGPAPRRPWERGDGQDVSARQAFQAAKIITYKDPDNPEYLEFLKQLKHLAYEQFNFTMEDVLVNTIP; ASFHDGLLLYIQAVTETLAHGGTVTDGENITQRMWNRSFQGVTGYLKIDSSGDRETDFSLWDMDPENGAF; RVVLNYNGTSQELVAVSGRKLNWPLGYPPPDIPKCGFDNEDPACNQDHLSTLEVLALVGSLSLLGILIVS; FFIYRKMQLEKELASELWRVRWEDVEPSSLERHLRSAGSRLTLSGRGSNYGSLLTTEGQFQVFAKTAYYK; GNLVAVKRVNRKRIELTRKVLFELKHMRDVQNEHLTRFVGACTDPPNICILTEYCPRGSLQDILENESIT; LDWMFRYSLTNDIVKGMLFLHNGAICSHGNLKSSNCVVDGRFVLKITDYGLESFRDLDPEQGHTVYAKKL; WTAPELLRMASPPVRGSQAGDVYSFGIILQEIALRSGVFHVEGLDLSPKEIIERVTRGEQPPFRPSLALQ; SHLEELGLLMQRCWAEDPQERPPFQQIRLTLRKFNRENSSNILDNLLSRMEQYANNLEELVEERTQAYLE; EKRKAEALLYQILPHSVAEQLKRGETVQAEAFDSVTIYFSDIVGFTALSAESTPMQVVTLLNDLYTCFDA; VIDNFDVYKVETIGDAYMVVSGLPVRNGRLHACEVARMALALLDAVRSFRIRHRPQEQLRLRIGIHTGPV; CAGVVGLKMPRYCLFGDTVNTASRMESNGEALKIHLSSETKAVLEEFGGFELELRGDVEMKGKGKVRTYW; LLGERGSSTRG; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000906
• ORF Size: 3183 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000906.2, NP_000897.2
• RefSeq Size: 4201 bp
• RefSeq ORF: 3186 bp
• Locus ID: 4881
• UniProt ID: P16066
• Cytogenetics: 1q21.3
• Domains: ANF_receptor, CYCc, pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Purine metabolism, Vascular smooth muscle contraction
• MW: 119 kDa
• Summary: Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009]