SREBP2 (SREBF2) (NM_004599) Human Tagged ORF Clone

CAT#: RC208942

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SREBF2 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 2 (SREBF2)



  "NM_004599" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,360.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SREBF2
Synonyms bHLHd2; SREBP-2; SREBP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208942 representing NM_004599
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGACAGCGGCGAGCTGGGTGGTCTGGAGACCATGGAGACCCTCACGGAGCTGGGCGACGAGCTGA
CCCTGGGAGACATCGACGAGATGCTGCAATTTGTCAGTAATCAAGTGGGAGAGTTCCCTGACTTGTTTTC
AGAACAGCTGTGTAGCTCCTTTCCTGGCAGTGGTGGTAGTGGTAGCAGCAGCGGCAGCAGTGGCAGCAGC
AGCAGCAGCAGCAATGGCAGGGGCAGCAGCAGCGGAGCTGTGGACCCTTCAGTGCAACGGTCATTCACCC
AGGTCACATTACCTTCCTTCTCTCCCTCGGCGGCCTCCCCACAGGCTCCAACTCTGCAAGTCAAGGTTTC
TCCCACCTCAGTTCCCACCACACCCAGGGCAACTCCTATTCTTCAGCCCCGCCCCCAGCCCCAGCCTCAA
CCTCAAACTCAGCTGCAACAACAGACGGTAATGATCACGCCAACATTCAGCACCACTCCGCAGACGAGGA
TCATCCAGCAGCCTTTGATATACCAGAATGCAGCTACTAGCTTTCAAGTCCTTCAGCCTCAAGTCCAAAG
CCTGGTGACATCCTCCCAGGTACAGCCGGTCACCATTCAGCAGCAGGTGCAGACAGTACAGGCCCAGCGG
GTGCTGACACAAACGGCCAATGGCACGCTGCAGACCCTTGCCCCGGCTACGGTGCAGACAGTTGCTGCGC
CACAGGTGCAGCAGGTCCCGGTCCTGGTCCAGCCTCAGATCATCAAGACAGATTCCCTTGTTTTGACCAC
ACTGAAGACAGATGGCAGCCCTGTTATGGCTGCGGTCCAGAACCCGGCCCTCACCGCCCTCACCACCCCT
ATCCAGACGGCTGCCCTTCAAGTACCAACCCTGGTGGGCAGCAGTGGGACCATTCTGACCACAATGCCTG
TAATGATGGGGCAAGAGAAAGTGCCCATTAAGCAGGTACCTGGGGGAGTCAAGCAGCTTGAGCCCCCCAA
AGAAGGAGAAAGGCGGACAACCCATAATATCATTGAGAAACGATATCGCTCCTCCATCAATGACAAAATC
ATCGAATTGAAAGACCTGGTCATGGGGACAGACGCCAAGATGCACAAGTCTGGCGTTCTGAGGAAGGCCA
TTGATTACATCAAATACTTGCAGCAGGTCAATCATAAACTGCGCCAGGAGAACATGGTGCTGAAGCTGGC
AAATCAAAAGAACAAGCTTCTAAAGGGCATCGACCTAGGCAGTCTGGTGGACAATGAGGTGGACCTGAAG
ATCGAGGACTTTAATCAGAATGTCCTTCTGATGTCCCCCCCAGCCTCTGACTCAGGGTCCCAGGCTGGCT
TCTCTCCCTACTCCATTGACTCTGAGCCAGGAAGCCCTCTATTGGATGATGCAAAGGTCAAAGATGAGCC
AGACTCTCCTCCTGTGGCGCTGGGCATGGTAGACCGCTCACGGATTCTTCTGTGTGTCCTCACCTTCCTG
TGCCTCTCCTTTAACCCCCTGACTTCCCTGCTGCAGTGGGGAGGGGCCCACGACTCTGACCAGCACCCAC
ACTCAGGCTCTGGCCGCAGTGTCCTGTCATTCGAGTCAGGTTCTGGGGGCTGGTTTGACTGGATGATGCC
TACTCTTCTCTTATGGCTGGTAAATGGTGTGATTGTCCTGAGCGTCTTTGTGAAGCTTCTGGTTCATGGG
GAGCCAGTGATCCGGCCACACTCGCGCTCCTCGGTCACCTTCTGGAGGCACCGGAAACAGGCAGATCTGG
ATCTCGCCAGAGGAGATTTTGCAGCTGCTGCCGGCAACCTACAAACCTGCCTGGCAGTTTTGGGCCGGGC
ACTGCCCACCTCCCGCCTGGACCTGGCCTGCAGCCTCTCCTGGAACGTGATCCGCTACAGCCTGCAGAAG
CTACGCCTGGTGCGCTGGCTGCTCAAGAAAGTCTTCCAGTGCCGGCGGGCCACGCCAGCCACTGAGGCAG
GCTTTGAAGACGAAGCTAAGACCAGCGCCCGGGATGCGGCTCTGGCCTATCACCGGCTGCACCAGCTGCA
CATCACAGGGAAGCTTCCTGCAGGATCCGCCTGTTCCGATGTACACATGGCGTTGTGTGCCGTGAACCTG
GCTGAATGTGCAGAGGAGAAGATCCCACCGAGCACACTGGTTGAGATCCATCTGACTGCTGCCATGGGGC
TCAAGACCCGGTGTGGAGGCAAGCTGGGCTTCCTGGCCAGCTACTTCCTCAGCCGAGCCCAGAGCCTGTG
TGGCCCCGAGCACAGTGCTGTTCCTGACTCCCTGCGCTGGCTCTGCCACCCCCTGGGCCAGAAGTTTTTC
ATGGAGCGGAGCTGGTCTGTGAAGTCAGCTGCCAAGGAGAGTCTATACTGTGCCCAGAGGAACCCAGCTG
ACCCCATTGCGCAGGTCCACCAGGCCTTCTGCAAGAACCTGCTGGAGCGAGCTATAGAGTCCTTGGTGAA
ACCTCAGGCCAAGAAGAAGGCTGGAGACCAGGAAGAAGAGAGCTGTGAATTCTCCAGTGCTCTGGAGTAC
TTGAAATTACTTCATTCTTTTGTGGACTCTGTGGGGGTTATGAGCCCCCCACTCTCCAGGAGCTCCGTGC
TCAAGTCCGCCCTGGGTCCAGACATCATCTGTCGGTGGTGGACGTCTGCAATCACTGTGGCCATCAGCTG
GCTCCAGGGAGACGATGCAGCTGTGCGCTCTCATTTTACCAAAGTGGAACGCATCCCCAAGGCCCTGGAA
GTGACAGAGAGCCCCCTGGTGAAGGCCATCTTCCATGCCTGCAGAGCCATGCATGCCTCACTCCCTGGGA
AAGCAGATGGGCAGCAGAGTTCCTTCTGCCATTGCGAGAGGGCCAGTGGCCACCTATGGAGCAGCCTCAA
CGTCAGTGGGGCCACCTCTGACCCTGCCCTCAACCACGTGGTCCAGCTGCTCACCTGTGACCTGCTACTG
TCGCTACGGACAGCGCTCTGGCAAAAACAGGCCAGTGCCAGCCAGGCTGTGGGGGAGACCTACCACGCGT
CAGGCGCTGAACTGGCGGGCTTCCAACGGGACCTGGGCAGCCTGCGCAGGCTGGCACACAGCTTCCGCCC
AGCATACCGCAAGGTGTTCCTGCATGAAGCCACCGTGCGCCTGATGGCAGGAGCCAGCCCCACCCGCACC
CACCAGCTGCTGGAACACAGCCTGCGGCGGCGCACCACGCAGAGCACCAAGCACGGAGAGGTGGATGCCT
GGCCCGGCCAGCGAGAGCGGGCCACCGCCATCCTGCTGGCCTGCCGCCACCTGCCCCTCTCCTTCCTCTC
CTCCCCGGGCCAGCGGGCAGTGCTGCTGGCCGAAGCTGCCCGCACCCTGGAGAAGGTGGGCGACCGGCGC
TCCTGCAACGACTGCCAGCAGATGATTGTTAAGCTGGGTGGTGGCACTGCCATTGCCGCCTCC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC208942 representing NM_004599
Red=Cloning site Green=Tags(s)

MDDSGELGGLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGSGGSGSSSGSSGSS
SSSSNGRGSSSGAVDPSVQRSFTQVTLPSFSPSAASPQAPTLQVKVSPTSVPTTPRATPILQPRPQPQPQ
PQTQLQQQTVMITPTFSTTPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQR
VLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTP
IQTAALQVPTLVGSSGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKI
IELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNEVDLK
IEDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFL
CLSFNPLTSLLQWGGAHDSDQHPHSGSGRSVLSFESGSGGWFDWMMPTLLLWLVNGVIVLSVFVKLLVHG
EPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAGNLQTCLAVLGRALPTSRLDLACSLSWNVIRYSLQK
LRLVRWLLKKVFQCRRATPATEAGFEDEAKTSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNL
AECAEEKIPPSTLVEIHLTAAMGLKTRCGGKLGFLASYFLSRAQSLCGPEHSAVPDSLRWLCHPLGQKFF
MERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEY
LKLLHSFVDSVGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFTKVERIPKALE
VTESPLVKAIFHACRAMHASLPGKADGQQSSFCHCERASGHLWSSLNVSGATSDPALNHVVQLLTCDLLL
SLRTALWQKQASASQAVGETYHASGAELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRT
HQLLEHSLRRRTTQSTKHGEVDAWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRR
SCNDCQQMIVKLGGGTAIAAS

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004599
ORF Size 3423 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004599.4
RefSeq Size 4325 bp
RefSeq ORF 3426 bp
Locus ID 6721
UniProt ID Q12772
Cytogenetics 22q13.2
Domains HLH
Protein Families Druggable Genome, Transcription Factors
MW 123.5 kDa
Gene Summary This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

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