SREBP2 (SREBF2) (NM_004599) Human Untagged Clone

CAT#: SC127910

SREBF2 (untagged)-Human sterol regulatory element binding transcription factor 2 (SREBF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SREBP2
• Synonyms: bHLHd2; SREBP-2; SREBP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127910 sequence for NM_004599 edited (data generated by NextGen Sequencing)
  ATGGACGACAGCGGCGAGCTGGGTGGTCTGGAGACCATGGAGACCCTCACGGAGCTGGGC
  GACGAGCTGACCCTGGGAGACATCGACGAGATGCTGCAATTTGTCAGTAATCAAGTGGGA
  GAGTTCCCTGACTTGTTTTCAGAACAGCTGTGTAGCTCCTTTCCTGGCAGTGGTGGTAGT
  GGTAGCAGCAGCGGCAGCAGTGGCAGCAGCAGCAGCAGCAGCAATGGCAGGGGCAGCAGC
  AGCGGAGCTGTGGACCCTTCAGTGCAACGGTCATTCACCCAGGTCACATTACCTTCCTTC
  TCTCCCTCGGCGGCCTCCCCACAGGCTCCAACTCTGCAAGTCAAGGTTTCTCCCACCTCA
  GTTCCCACCACACCCAGGGCAACTCCTATTCTTCAGCCCCGCCCCCAGCCCCAGCCTCAA
  CCTCAAACTCAGCTGCAACAACAGACGGTAATGATCACGCCAACATTCAGCACCACTCCG
  CAGACGAGGATCATCCAGCAGCCTTTGATATACCAGAATGCAGCTACTAGCTTTCAAGTC
  CTTCAGCCTCAAGTCCAAAGCCTGGTGACATCCTCCCAGGTACAGCCGGTCACCATTCAG
  CAGCAGGTGCAGACAGTACAGGCCCAGCGGGTGCTGACACAAACGGCCAATGGCACGCTG
  CAGACCCTTGCCCCGGCTACGGTGCAGACAGTTGCTGCGCCACAGGTGCAGCAGGTCCCG
  GTCCTGGTCCAGCCTCAGATCATCAAGACAGATTCCCTTGTTTTGACCACACTGAAGACA
  GATGGCAGCCCTGTTATGGCTGCGGTCCAGAACCCGGCCCTCACCGCCCTCACCACCCCT
  ATCCAGACGGCTGCCCTTCAAGTACCAACCCTGGTGGGCAGCAGTGGGACCATTCTGACC
  ACAATGCCTGTAATGATGGGGCAAGAGAAAGTGCCCATTAAGCAGGTACCTGGGGGAGTC
  AAGCAGCTTGAGCCCCCCAAAGAAGGAGAAAGGCGGACAACCCATAATATCATTGAGAAA
  CGATATCGCTCCTCCATCAATGACAAAATCATCGAATTGAAAGACCTGGTCATGGGGACA
  GACGCCAAGATGCACAAGTCTGGCGTTCTGAGGAAGGCCATTGATTACATCAAATACTTG
  CAGCAGGTCAATCATAAACTGCGCCAGGAGAACATGGTGCTGAAGCTGGCAAATCAAAAG
  AACAAGCTTCTAAAGGGCATCGACCTAGGCAGTCTGGTGGACAATGAGGTGGACCTGAAG
  ATCGAGGACTTTAATCAGAATGTCCTTCTGATGTCCCCCCCAGCCTCTGACTCAGGGTCC
  CAGGCTGGCTTCTCTCCCTACTCCATTGACTCTGAGCCAGGAAGCCCTCTATTGGATGAT
  GCAAAGGTCAAAGATGAGCCAGACTCTCCTCCTGTGGCGCTGGGCATGGTAGACCGCTCA
  CGGATTCTTCTGTGTGTCCTCACCTTCCTGTGCCTCTCCTTTAACCCCCTGACTTCCCTG
  CTGCAGTGGGGAGGGGCCCACGACTCTGACCAGCACCCACACTCAGGCTCTGGCCGCAGT
  GTCCTGTCATTCGAGTCAGGTTCTGGGGGCTGGTTTGACTGGATGATGCCTACTCTTCTC
  TTATGGCTGGTAAATGGTGTGATTGTCCTGAGCGTCTTTGTGAAGCTGCTGGTTCATGGG
  GAGCCAGTGATCCGGCCACACTCGCGCTCCTCGGTCACCTTCTGGAGGCACCGGAAACAG
  GCAGATCTGGATCTCGCCAGAGGAGATTTTGCAGCTGCTGCCGGCAACCTACAAACCTGC
  CTGGCAGTTTTGGGCCGGGCACTGCCCACCTCCCGCCTGGACCTGGCCTGCAGCCTCTCC
  TGGAACGTGATCCGCTACAGCCTGCAGAAGCTACGCCTGGTGCGCTGGCTGCTCAAGAAA
  GTCTTCCAGTGCCGGCGGGCCACGCCAGCCACTGAGGCAGGCTTTGAAGACGAAGCTAAG
  ACCAGCGCCCGGGATGCGGCTCTGGCCTATCACCGGCTGCACCAGCTGCACATCACAGGG
  AAGCTTCCTGCAGGATCCGCCTGTTCCGATGTACACATGGCGTTGTGTGCCGTGAACCTG
  GCTGAATGTGCAGAGGAGAAGATCCCACCGAGCACACTGGTTGAGATCCATCTGACTGCT
  GCCATGGGGCTCAAGACCCGGTGTGGAGGCAAGCTGGGCTTCCTGGCCAGCTACTTCCTC
  AGCCGAGCCCAGAGCCTGTGTGGCCCCGAGCACAGTGCTGTTCCTGACTCCCTGCGCTGG
  CTCTGCCACCCCCTGGGCCAGAAGTTTTTCATGGAGCGGAGCTGGTCTGTGAAGTCAGCT
  GCCAAGGAGAGTCTATACTGTGCCCAGAGGAACCCAGCTGACCCCATTGCGCAGGTCCAC
  CAGGCCTTCTGCAAGAACCTGCTGGAGCGAGCTATAGAGTCCTTGGTGAAACCTCAGGCC
  AAGAAGAAGGCTGGAGACCAGGAAGAAGAGAGCTGTGAATTCTCCAGTGCTCTGGAGTAC
  TTGAAATTACTTCATTCTTTTGTGGACTCTGTGGGGGTTATGAGCCCCCCACTCTCCAGG
  AGCTCCGTGCTCAAGTCCGCCCTGGGTCCAGACATCATCTGTCGGTGGTGGACGTCTGCA
  ATCACTGTGGCCATCAGCTGGCTCCAGGGAGACGATGCAGCTGTGCGCTCTCATTTTACC
  AAAGTGGAACGCATCCCCAAGGCCCTGGAAGTGACAGAGAGCCCCCTGGTGAAGGCCATC
  TTCCATGCCTGCAGAGCCATGCATGCCTCACTCCCTGGGAAAGCAGATGGGCAGCAGAGT
  TCCTTCTGCCATTGCGAGAGGGCCAGTGGCCACCTATGGAGCAGCCTCAACGTCAGTGGG
  GCCACCTCTGACCCTGCCCTCAACCACGTGGTCCAGCTGCTCACCTGTGACCTGCTACTG
  TCGCTACGGACAGCGCTCTGGCAAAAACAGGCCAGTGCCAGCCAGGCTGTGGGGGAGACC
  TACCACGCGTCAGGCGCTGAACTGGCGGGCTTCCAACGGGACCTGGGCAGCCTGCGCAGG
  CTGGCACACAGCTTCCGCCCAGCATACCGCAAGGTGTTCCTGCATGAAGCCACCGTGCGC
  CTGATGGCAGGAGCCAGCCCCACCCGCACCCACCAGCTGCTGGAACACAGCCTGCGGCGG
  CGCACCACGCAGAGCACCAAGCACGGAGAGGTGGATGCCTGGCCCGGCCAGCGAGAGCGG
  GCCACCGCCATCCTGCTGGCCTGCCGCCACCTGCCCCTCTCCTTCCTCTCCTCCCCGGGC
  CAGCGGGCAGTGCTGCTGGCCGAAGCTGCCCGCACCCTGGAGAAGGTGGGCGACCGGCGC
  TCCTGCAACGACTGCCAGCAGATGATTGTTAAGCTGGGTGGTGGCACTGCCATTGCCGCC
  TCCTGA
  
  Clone variation with respect to NM_004599.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004599 unedited
  TTTGGTATACGACTCAATATAGGGCGGCCGCGATATCGGCACGAGTGCGCAACCATGGCG
  GCGGGTGGCAACCCGTCGGTGAGGCGGTGCCGGGCGGGGGTTGTCGGGTGTCATTGGGCG
  GTGGCGACGGCACCGCCCCCGCGTCTCCCTTGAGCGGGACGGCAGGGGGGGCTTCTGCGC
  TGAGCCGGGCGATGGACGACAGCGGCGAGCTGGGTGGTCTGGAGACCATGGAGACCCTCA
  CGGAGCTGGGCGACGAGCTGACCCTGGGAGACATCGACGAGATGCTGCAATNTTGTCAGT
  AANTCAAGTGGGAGAGTTCCCTGACTTGTTTTCAGAACAGCTGTGTAGCTCCTTTCCTGG
  CAGTGGTGGTTAGTGGTAGCAGCAGCGGCAGCAGTGGCAGCAGCAGCAGCAGCAGCAATG
  GCAGGGGCAGCAGCAGCGGGAGCTGTGGACCCTTCATGNCAACGGTCATTCACCCAGGTC
  ACATTACCTTCCTTCTCTCCCTCGGCGGCCTCCCCACAGGCTCCAACTCTGCAAGTAAAG
  GTTTCTCCCACCTCAGTTCCCACCACACCCAGGGCAACTCCTATTCTTCAGCCCCGCCCC
  CAGCCCCAGCCTCAACCTCAAACTCAGCTGCAACAACAGACGGGNNATGATCACGCCAAC
  ATTCAGCACCACTCCGCAGACCGAGGATCATCCANCAGCCTTTGATATACCAGATGCAGC
  TACTAGCTTTTCAAGTCTTTAGCCTCAGTCCAAAGCCTGGTGGACATCTCCAGTAACAAG
  CCGTCACCATAACAACAGGGGCAACAGTCAGCCCCCCGGGGGGGCTAACACAACGGCCAT
  GGACGCTTGAAACCTTTGCCGGNTAGGGGGAAAAAATTGCTCCCCAGGGCACAAAAACCC
  CGCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004599
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004599.2, NP_004590.2
• RefSeq Size: 4325 bp
• RefSeq ORF: 3426 bp
• Locus ID: 6721
• UniProt ID: Q12772
• Cytogenetics: 22q13.2
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.