C6orf134 (ATAT1) (NM_001031722) Human Tagged ORF Clone

SKU
RC208373
ATAT1 (Myc-DDK-tagged)-Human alpha tubulin acetyltransferase 1 (ATAT1), transcript variant 1
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$457.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C6orf134
Synonyms alpha-TAT; alpha-TAT1; C6orf134; MEC17; Nbla00487; TAT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208373 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGTTGACCTGGCCTTTCTGCTTCCTCACAATAACCTTAAGGGAGGAGGGAGTGTGCCACCTTGAAA
GTGTTGATCTACAGCAGCAAATTATGACCATTATAGATGAACTGGGCAAGGCTTCTGCCAAGGCCCAGAA
TCTTTCCGCTCCTATCACTAGTGCATCAAGGATGCAGAGTAACCGCCATGTTGTTTATATTCTCAAAGAC
AGTTCAGCCCGACCGGCTGGAAAAGGAGCCATTATTGGTTTCATCAAAGTTGGATACAAGAAGCTCTTTG
TACTGGATGATCGTGAGGCTCATAATGAGGTAGAACCACTTTGCATCCTGGACTTTTACATCCATGAGTC
TGTGCAACGCCATGGCCATGGGCGAGAACTCTTCCAGTATATGTTGCAGAAGGAGCGAGTGGAACCGCAC
CAACTGGCAATTGACCGACCCTCACAGAAGCTGCTGAAATTCCTGAATAAGCACTACAATCTGGAGACCA
CAGTCCCACAGGTGAACAACTTTGTGATCTTTGAAGGCTTCTTTGCCCATCAACATCGGCCCCCTGCTCC
CTCTCTGAGGGCAACTCGACACTCTCGTGCTGCTGCAGTCGATCCCACGCCCGCTGCTCCAGCAAGGAAG
CTGCCACCCAAGAGAGCAGAGGGAGACATCAAGCCATACTCCTCTAGTGACCGAGAATTTCTGAAGGTAG
CTGTGGAGCCTCCTTGGCCCCTAAACAGGGCCCCTCGCCGCGCCACACCTCCAGCCCACCCACCCCCCCG
CTCCAGCAGCCTGGGAAACTCACCAGAACGAGGTCCCCTCCGCCCCTTTGTGCCAGAGCAGGAGCTGCTG
CGTTCCTTGCGCCTCTGCCCCCCACACCCTACCGCCCGCCTTCTGTTGGCTGCTGACCCTGGGGGCAGCC
CAGCTCAACGTCGTCGCACCAGGGGGACTCCCCCAGGTCTGGTAGCCCAAAGCTGCTGCTACAGCCGCCA
TGGGGGGGTGAATTCCTCATCCCCCAATACAGGCAACCAAGACTCCAAGCAGGGAGAACAGGAAACAAAG
AATAGGTCTGCCAGTGAGGAGCAGGCCTTGTCACAGGATGGGTCTGGGGAGAAGCCCATGCACACAGCTC
CTCCACAGGCCCCGGCCCCGCCAGCCCAGTCCTGGACAGTGGGTGGGGACATACTCAACGCCAGGTTCAT
TCGAAACCTGCAGGAACGTCGCAGCACCAGGCCTTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208373 protein sequence
Red=Cloning site Green=Tags(s)

MWLTWPFCFLTITLREEGVCHLESVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKD
SSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPH
QLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPAAPARK
LPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELL
RSLRLCPPHPTARLLLAADPGGSPAQRRRTRGTPPGLVAQSCCYSRHGGVNSSSPNTGNQDSKQGEQETK
NRSASEEQALSQDGSGEKPMHTAPPQAPAPPAQSWTVGGDILNARFIRNLQERRSTRPW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001031722
ORF Size 1227 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001031722.4
RefSeq Size 1740 bp
RefSeq ORF 1230 bp
Locus ID 79969
UniProt ID Q5SQI0
Cytogenetics 6p21.33
MW 45.6 kDa
Summary This gene encodes a protein that localizes to clathrin-coated pits, where it acetylates alpha tubulin on lysine 40. This process may be important in microtubule growth, for instance during chemotaxis and the formation of cilium. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
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SKU Description Size Price
RC208373L1 Lenti ORF clone of Human alpha tubulin acetyltransferase 1 (ATAT1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC208373L2 Lenti ORF clone of Human alpha tubulin acetyltransferase 1 (ATAT1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC208373L3 Lenti ORF clone of Human alpha tubulin acetyltransferase 1 (ATAT1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC208373L4 Lenti ORF clone of Human alpha tubulin acetyltransferase 1 (ATAT1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG208373 ATAT1 (tGFP-tagged) - Human alpha tubulin acetyltransferase 1 (ATAT1), transcript variant 1 10 ug
$489.00 MSRP $657.00 MSRP $657.00
SC321825 ATAT1 (untagged)-Human alpha tubulin acetyltransferase 1 (ATAT1), transcript variant 1 10 ug
$457.00

Citations

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