Ribonuclease Inhibitor (RNH1) (NM_002939) Human Tagged ORF Clone

SKU RC208360

RNH1 (Myc-DDK-tagged)-Human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Ribonuclease Inhibitor
• Synonyms: RAI; RNH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208360 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCCTGGACATCCAGAGCCTGGACATCCAGTGTGAGGAGCTGAGCGACGCTAGATGGGCCGAGCTCC; TCCCTCTGCTCCAGCAGTGCCAAGTGGTCAGGCTGGACGACTGTGGCCTCACGGAAGCACGGTGCAAGGA; CATCAGCTCTGCACTTCGAGTCAACCCTGCACTGGCAGAGCTCAACCTGCGCAGCAACGAGCTGGGCGAT; GTCGGCGTGCATTGCGTGCTCCAGGGCCTGCAGACCCCCTCCTGCAAGATCCAGAAGCTGAGCCTCCAGA; ACTGCTGCCTGACGGGGGCCGGCTGCGGGGTCCTGTCCAGCACACTACGCACCCTGCCCACCCTGCAGGA; GCTGCACCTCAGCGACAACCTCTTGGGGGATGCGGGCCTGCAGCTGCTCTGCGAAGGACTCCTGGACCCC; CAGTGCCGCCTGGAAAAGCTGCAGCTGGAGTATTGCAGCCTCTCGGCTGCCAGCTGCGAGCCCCTGGCCT; CCGTGCTCAGGGCCAAGCCGGACTTCAAGGAGCTCACGGTTAGCAACAACGACATCAATGAGGCTGGCGT; CCGTGTGCTGTGCCAGGGCCTGAAGGACTCCCCCTGCCAGCTGGAGGCGCTCAAGCTGGAGAGCTGCGGT; GTGACATCAGACAACTGCCGGGACCTGTGCGGCATTGTGGCCTCCAAGGCCTCGCTGCGGGAGCTGGCCC; TGGGCAGCAACAAGCTGGGTGATGTGGGCATGGCGGAGCTGTGCCCAGGGCTGCTCCACCCCAGCTCCAG; GCTCAGGACCCTGTGGATCTGGGAGTGTGGCATCACTGCCAAGGGCTGCGGGGATCTGTGCCGTGTCCTC; AGGGCCAAGGAGAGCCTGAAGGAGCTCAGCCTGGCCGGCAACGAGCTGGGGGATGAGGGTGCCCGACTGC; TGTGTGAGACCCTGCTGGAACCTGGCTGCCAGCTGGAGTCGCTGTGGGTGAAGTCCTGCAGCTTCACAGC; CGCCTGCTGCTCCCACTTCAGCTCAGTGCTGGCCCAGAACAGGTTTCTCCTGGAGCTACAGATAAGCAAC; AACAGGCTGGAGGATGCGGGCGTGCGGGAGCTGTGCCAGGGCCTGGGCCAGCCTGGCTCTGTGCTGCGGG; TGCTCTGGTTGGCCGACTGCGATGTGAGTGACAGCAGCTGCAGCAGCCTCGCCGCAACCCTGTTGGCCAA; CCACAGCCTGCGTGAGCTGGACCTCAGCAACAACTGCCTGGGGGACGCCGGCATCCTGCAGCTGGTGGAG; AGCGTCCGGCAGCCGGGCTGCCTCCTGGAGCAGCTGGTCCTGTACGACATTTACTGGTCTGAGGAGATGG; AGGACCGGCTGCAGGCCCTGGAGAAGGACAAGCCATCCCTGAGGGTCATCTCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208360 protein sequence; Red=Cloning site Green=Tags(s); ; MSLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGD; VGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDP; QCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCG; VTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVL; RAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISN; NRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVE; SVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002939
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002939.4
• RefSeq Size: 2057 bp
• RefSeq ORF: 1386 bp
• Locus ID: 6050
• UniProt ID: P13489
• Cytogenetics: 11p15.5
• Domains: LRR, LRR_RI
• MW: 50 kDa
• Summary: Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]