TAX1BP1 (NM_006024) Human Tagged ORF Clone

SKU RC208233

TAX1BP1 (Myc-DDK-tagged)-Human Tax1 (human T-cell leukemia virus type I) binding protein 1 (TAX1BP1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TAX1BP1
• Synonyms: CALCOCO3; T6BP; TXBP151
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208233 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACATCCTTTCAAGAAGTCCCATTGCAGACTTCCAACTTTGCCCATGTCATCTTTCAAAATGTGGCCA; AGAGTTACCTTCCTAATGCACACCTGGAATGTCATTACACCTTAACTCCATATATTCATCCACATCCAAA; AGATTGGGTTGGTATATTCAAGGTTGGATGGAGTACTGCTCGTGATTATTACACGTTTTTATGGTCCCCT; ATGCCTGAACATTATGTGGAAGGATCAACAGTCAATTGTGTACTAGCATTCCAAGGATATTACCTTCCAA; ATGATGATGGAGAATTTTATCAGTTCTGTTACGTTACCCATAAGGGTGAAATTCGTGGAGCAAGTACACC; TTTCCAGTTTCGAGCTTCTTCTCCAGTTGAAGAGCTGCTTACTATGGAAGATGAAGGAAATTCTGACATG; TTAGTGGTGACCACAAAAGCAGGCCTTCTTGAGTTGAAAATTGAGAAAACCATGAAAGAAAAAGAAGAAC; TGTTAAAGTTAATTGCCGTTCTGGAAAAAGAAACAGCACAACTTCGAGAACAAGTTGGGAGAATGGAAAG; AGAACTTAACCATGAGAAAGAAAGATGTGACCAACTGCAAGCAGAACAAAAGGGTCTTACTGAAGTAACA; CAAAGCTTAAAAATGGAAAATGAAGAGTTTAAGAAGAGGTTCAGTGATGCTACATCCAAAGCCCATCAGC; TTGAGGAAGATATTGTGTCAGTAACACATAAAGCAATTGAAAAAGAAACCGAATTAGACAGTTTAAAGGA; CAAACTCAAGAAGGCACAACATGAAAGAGAACAACTTGAATGTCAGTTGAAGACAGAGAAGGATGAAAAG; GAACTTTATAAGGTACATTTGAAGAATACAGAAATAGAAAATACCAAGCTTATGTCAGAGGTCCAGACTT; TAAAAAATATAGATGGGAACAAAGAAAGCGTGATTACTCATTTCAAAGAAGAGATTGGCAGGCTGCAGTT; ATGTTTGGCTGAAAAGGAAAATCTGCAAAGAACTTTCCTGCTTACAACCTCAAGTAAAGAAGATACTTGT; TTTTTAAAGGAGCAACTTCGTAAAGCAGAGGAACAGGTTCAGGCAACTCGGCAAGAAGTTGTCTTTCTGG; CTAAAGAACTCAGTGATGCTGTCAACGTACGAGACAGAACGATGGCAGACCTGCATACTGCACGCTTGGA; AAACGAGAAAGTGAAAAAGCAGTTAGCTGATGCAGTGGCAGAACTTAAACTAAATGCTATGAAAAAAGAT; CAGGACAAGACTGATACACTGGAACACGAACTAAGAAGAGAAGTTGAAGATCTGAAACTCCGTCTTCAGA; TGGCTGCAGACCATTATAAAGAAAAATTTAAGGAATGCCAAAGGCTCCAAAAACAAATAAACAAACTTTC; AGATCAATCAGCTAATAATAATAATGTCTTCACAAAGAAAACGGGGAATCAGCAGAAAGTGAATGATGCT; TCAGTAAACACAGACCCAGCCACTTCTGCCTCTACTGTAGATGTAAAGCCATCACCTTCTGCAGCAGAGG; CAGATTTTGACATAGTAACAAAGGGGCAAGTCTGTGAAATGACCAAAGAAATTGCTGACAAAACAGAAAA; GTATAATAAATGTAAACAACTCTTGCAGGATGAGAAAGCAAAATGCAATAAATATGCTGATGAACTTGCA; AAAATGGAGCTGAAATGGAAAGAACAAGTGAAAATTGCTGAAAATGTAAAACTTGAACTAGCTGAAGTAC; AGGACAATTATAAAGAACTTAAAAGGAGTCTAGAAAATCCAGCAGAAAGGAAAATGGAAGGTCAGAATTC; CCAGAGTCCTCAATGTTTCAAAACATGCTCAGAGCAAAATGGTTATGTTCTCACATTGTCAAATGCACAA; CCAGTTCTGCAATATGGTAATCCTTATGCATCTCAGGAAACAAGAGATGGAGCAGATGGTGCTTTTTACC; CAGATGAAATACAAAGGCCACCTGTCAGAGTCCCCTCTTGGGGACTGGAAGACAATGTTGTCTGCAGCCA; GCCTGCTCGAAACTTTAGTCGGCCTGATGGCTTAGAGGACTCTGAGGATAGCAAAGAAGATGAGAATGTG; CCTACTGCTCCTGATCCTCCAAGTCAACATTTACGTGGGCATGGGACAGGCTTTTGCTTTGATTCCAGCT; TTGATGTTCACAAGAAGTGTCCCCTCTGTGAGTTAATGTTTCCTCCTAACTATGATCAGAGCAAATTTGA; AGAACATGTTGAAAGTCACTGGAAGGTGTGCCCGATGTGCAGCGAGCAGTTCCCTCCTGACTATGACCAG; CAGGTGTTTGAAAGGCATGTGCAGACCCATTTTGATCAGAATGTTCTAAATTTTGAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208233 protein sequence; Red=Cloning site Green=Tags(s); ; MTSFQEVPLQTSNFAHVIFQNVAKSYLPNAHLECHYTLTPYIHPHPKDWVGIFKVGWSTARDYYTFLWSP; MPEHYVEGSTVNCVLAFQGYYLPNDDGEFYQFCYVTHKGEIRGASTPFQFRASSPVEELLTMEDEGNSDM; LVVTTKAGLLELKIEKTMKEKEELLKLIAVLEKETAQLREQVGRMERELNHEKERCDQLQAEQKGLTEVT; QSLKMENEEFKKRFSDATSKAHQLEEDIVSVTHKAIEKETELDSLKDKLKKAQHEREQLECQLKTEKDEK; ELYKVHLKNTEIENTKLMSEVQTLKNIDGNKESVITHFKEEIGRLQLCLAEKENLQRTFLLTTSSKEDTC; FLKEQLRKAEEQVQATRQEVVFLAKELSDAVNVRDRTMADLHTARLENEKVKKQLADAVAELKLNAMKKD; QDKTDTLEHELRREVEDLKLRLQMAADHYKEKFKECQRLQKQINKLSDQSANNNNVFTKKTGNQQKVNDA; SVNTDPATSASTVDVKPSPSAAEADFDIVTKGQVCEMTKEIADKTEKYNKCKQLLQDEKAKCNKYADELA; KMELKWKEQVKIAENVKLELAEVQDNYKELKRSLENPAERKMEGQNSQSPQCFKTCSEQNGYVLTLSNAQ; PVLQYGNPYASQETRDGADGAFYPDEIQRPPVRVPSWGLEDNVVCSQPARNFSRPDGLEDSEDSKEDENV; PTAPDPPSQHLRGHGTGFCFDSSFDVHKKCPLCELMFPPNYDQSKFEEHVESHWKVCPMCSEQFPPDYDQ; QVFERHVQTHFDQNVLNFD; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006024
• ORF Size: 2367 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006024.6
• RefSeq Size: 3506 bp
• RefSeq ORF: 2370 bp
• Locus ID: 8887
• UniProt ID: Q86VP1
• Cytogenetics: 7p15.2
• Protein Families: Druggable Genome
• MW: 90.9 kDa
• Summary: This gene encodes a HTLV-1 tax1 binding protein. The encoded protein interacts with TNFAIP3, and inhibits TNF-induced apoptosis by mediating the TNFAIP3 anti-apoptotic activity. Degradation of this protein by caspase-3-like family proteins is associated with apoptosis induced by TNF. This protein may also have a role in the inhibition of inflammatory signaling pathways. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]