Pellino 1 (PELI1) (NM_020651) Human Tagged ORF Clone

SKU RC208197

PELI1 (Myc-DDK-tagged)-Human pellino homolog 1 (Drosophila) (PELI1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Pellino 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208197 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTTTCTCCTGATCAAGAAAATCATCCATCTAAAGCACCAGTAAAATATGGTGAACTCATTGTCTTAG; GGTATAATGGGTCTCTCCCAAATGGCGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACC; TAAGGCAAATGGGGTGAAGCCCAGCACTGTGCATATTGCTTGTACTCCTCAGGCTGCAAAGGCAATAAGC; AACAAAGACCAGCATAGCATATCATATACTTTATCTCGGGCCCAGACTGTGGTGGTTGAATATACTCATG; ACAGCAACACCGATATGTTTCAGATTGGCCGGTCGACTGAAAGCCCCATTGATTTTGTAGTAACTGACAC; GGTTCCTGGAAGTCAAAGTAATTCTGATACACAGTCAGTACAAAGCACTATATCAAGATTTGCCTGCAGA; ATCATATGTGAACGGAATCCTCCCTTTACAGCACGGATTTATGCTGCAGGATTTGACTCATCAAAAAACA; TCTTTCTTGGGGAGAAGGCTGCCAAATGGAAGACATCAGATGGACAGATGGATGGCTTGACCACTAATGG; TGTTCTTGTGATGCATCCACGCAATGGGTTCACAGAAGACTCCAAGCCTGGAATATGGAGAGAAATATCG; GTGTGTGGAAATGTATTTAGCCTACGTGAAACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGGAAATTG; AAACCAATCAGTTACAAGATGGCTCGTTAATTGACCTCTGTGGTGCAACATTGTTATGGCGTACTGCAGA; AGGCCTTTCCCACACTCCTACCGTGAAGCATTTAGAAGCTTTAAGACAGGAAATCAATGCAGCACGACCT; CAGTGCCCTGTAGGGTTCAACACACTAGCATTTCCTAGTATGAAGAGGAAAGACGTTGTAGATGAAAAAC; AACCATGGGTATATCTAAACTGCGGCCATGTACATGGCTATCATAACTGGGGAAACAAAGAAGAACGTGA; TGGAAAAGATCGTGAATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTTCCTCTGTGGCTTGGATGTGAA; GCTGGATTTTATGTGGACGCCGGCCCTCCAACCCATGCGTTTAGCCCGTGTGGGCATGTGTGTTCAGAAA; AGACAACTGCCTATTGGTCCCAGATCCCACTTCCTCATGGTACTCATACTTTTCATGCAGCCTGTCCCTT; TTGTGCACATCAGTTGGCTGGTGAACAAGGCTACATCAGACTTATTTTTCAAGGACCTCTAGAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208197 protein sequence; Red=Cloning site Green=Tags(s); ; MFSPDQENHPSKAPVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAIS; NKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQSVQSTISRFACR; IICERNPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREIS; VCGNVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTPTVKHLEALRQEINAARP; QCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCE; AGFYVDAGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020651
• ORF Size: 1254 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020651.4
• RefSeq Size: 3780 bp
• RefSeq ORF: 1257 bp
• Locus ID: 57162
• UniProt ID: Q96FA3
• Cytogenetics: 2p14
• Domains: Pellino
• MW: 46.3 kDa
• Summary: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:12496252, PubMed:17675297). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (PubMed:29883609).[UniProtKB/Swiss-Prot Function]