ETV7 (NM_016135) Human Tagged ORF Clone

SKU
RC207742
ETV7 (Myc-DDK-tagged)-Human ets variant 7 (ETV7), transcript variant 1
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ETV7
Synonyms TEL-2; TEL2; TELB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207742 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGAGGGAGAATTGGCTATTTCTCCTATAAGCCCTGTGGCAGCCATGCCTCCCCTAGGCACCCACG
TGCAAGCCAGATGTGAAGCTCAAATTAACCTGCTGGGTGAAGGGGGGATCTGCAAGCTGCCAGGAAGACT
CCGCATCCAGCCCGCACTGTGGAGCAGGGAGGACGTGCTGCACTGGCTGCGCTGGGCAGAGCAGGAGTAC
TCTCTGCCATGCACCGCGGAGCACGGGTTCGAGATGAACGGACGCGCCCTCTGCATCCTCACCAAGGACG
ACTTCCGGCACCGTGCGCCCAGCTCAGGTGACGTCCTGTATGAGCTGCTCCAGTACATCAAGACCCAGCG
GCGAGCCCTGGTGTGTGGACCCTTTTTTGGAGGGATCTTCAGGCTGAAGACGCCCACCCAGCACTCTCCA
GTCCCCCCGGAAGAGGTGACTGGCCCCTCTCAGATGGACACCCGAAGGGGCCACCTGCTGCAGCCACCAG
ACCCAGGGCTTACCAGCAACTTCGGCCACCTGGATGACCCTGGCCTGGCAAGGTGGACCCCTGGCAAGGA
GGAGTCCCTCAACTTATGTCACTGTGCAGAGCTCGGCTGCAGGACCCAGGGGGTCTGTTCCTTCCCCGCG
ATGCCGCAGGCCCCCATTGACGGCAGGATCGCTGACTGCCGCCTGCTGTGGGATTACGTGTATCAGCTGC
TCCTTGATACCCGATATGAGCCCTACATCAAGTGGGAAGACAAGGACGCCAAGATCTTCCGAGTTGTGGA
TCCAAATGGGCTCGCCAGACTCTGGGGAAATCACAAGAACCGGGTGAACATGACCTACGAGAAGATGTCT
CGTGCCCTGCGCCACTATTATAAGCTTAATATCATTAAGAAGGAACCGGGGCAGAAACTCCTGTTCAGAT
TTCTAAAGACTCCGGGAAAGATGGTCCAGGACAAGCACAGCCACCTGGAGCCGCTGGAGAGCCAGGAGCA
GGACAGAATAGAGTTCAAGGACAAGAGGCCAGAAATCTCTCCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207742 protein sequence
Red=Cloning site Green=Tags(s)

MQEGELAISPISPVAAMPPLGTHVQARCEAQINLLGEGGICKLPGRLRIQPALWSREDVLHWLRWAEQEY
SLPCTAEHGFEMNGRALCILTKDDFRHRAPSSGDVLYELLQYIKTQRRALVCGPFFGGIFRLKTPTQHSP
VPPEEVTGPSQMDTRRGHLLQPPDPGLTSNFGHLDDPGLARWTPGKEESLNLCHCAELGCRTQGVCSFPA
MPQAPIDGRIADCRLLWDYVYQLLLDTRYEPYIKWEDKDAKIFRVVDPNGLARLWGNHKNRVNMTYEKMS
RALRHYYKLNIIKKEPGQKLLFRFLKTPGKMVQDKHSHLEPLESQEQDRIEFKDKRPEISP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_016135
ORF Size 1023 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016135.2, NP_057219.1
RefSeq Size 1761 bp
RefSeq ORF 1026 bp
Locus ID 51513
UniProt ID Q9Y603
Cytogenetics 6p21.31
Domains ETS, SAM_PNT, SAPA, SAPB, SapB_1, SapB_2
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Dorso-ventral axis formation
MW 39 kDa
Summary The protein encoded by this gene belongs to the ETS family of transcription factors, which is a large group of evolutionarily conserved transcriptional regulators that play an important role in a variety of cellular processes throughout development and differentiation, and are involved in oncogenesis as well. This protein is predominantly expressed in hematopoietic tissues. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene (PMID:11108721).[provided by RefSeq, May 2011]
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SKU Description Size Price
RC207742L1 Lenti ORF clone of Human ets variant 7 (ETV7), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC207742L2 Lenti ORF clone of Human ets variant 7 (ETV7), transcript variant 1, mGFP tagged 10 ug
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RC207742L3 Lenti ORF clone of Human ets variant 7 (ETV7), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC207742L4 Lenti ORF clone of Human ets variant 7 (ETV7), transcript variant 1, mGFP tagged 10 ug
$757.00
RG207742 ETV7 (tGFP-tagged) - Human ets variant 7 (ETV7), transcript variant 1 10 ug
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SC114475 ETV7 (untagged)-Human ets variant 7 (ETV7), transcript variant 1 10 ug
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Citations

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