Calcium independent Phospholipase A2 (PLA2G6) (NM_003560) Human Tagged ORF Clone

SKU RC207546

PLA2G6 (Myc-DDK-tagged)-Human phospholipase A2, group VI (cytosolic, calcium-independent) (PLA2G6), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Calcium independent Phospholipase A2
• Synonyms: CaI-PLA2; GVI; INAD1; iPLA2; IPLA2-VIA; iPLA2beta; NBIA2; NBIA2A; NBIA2B; PARK14; PLA2; PNPLA9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207546 representing NM_003560; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCAGTTCTTTGGCCGCCTGGTCAATACCTTCAGTGGCGTCACCAACTTGTTCTCTAACCCATTCCGGG; TGAAGGAGGTGGCTGTGGCCGACTACACCTCGAGTGACCGAGTTCGGGAGGAAGGGCAGCTGATTCTGTT; CCAGAACACTCCCAACCGCACCTGGGACTGCGTCCTGGTCAACCCCAGGAACTCACAGAGTGGATTCCGA; CTCTTCCAGCTGGAGTTGGAGGCTGACGCCCTAGTGAATTTCCATCAGTATTCTTCCCAGCTGCTACCCT; TCTATGAGAGCTCCCCTCAGGTCCTGCACACTGAGGTCCTGCAGCACCTGACCGACCTCATCCGTAACCA; CCCCAGCTGGTCAGTGGCCCACCTGGCTGTGGAGCTAGGGATCCGCGAGTGCTTCCATCACAGCCGTATC; ATCAGCTGTGCCAATTGCGCGGAGAACGAGGAGGGCTGCACACCCCTGCACCTGGCCTGCCGCAAGGGTG; ATGGGGAGATCCTGGTGGAGCTGGTGCAGTACTGCCACACTCAGATGGATGTCACCGACTACAAGGGAGA; GACCGTCTTCCATTATGCTGTCCAGGGTGACAATTCTCAGGTGCTGCAGCTCCTTGGAAGGAACGCAGTG; GCTGGCCTGAACCAGGTGAATAACCAAGGGCTGACCCCGCTGCACCTGGCCTGCCAGCTGGGGAAGCAGG; AGATGGTCCGCGTGCTGCTGCTGTGCAATGCTCGGTGCAACATCATGGGCCCCAACGGCTACCCCATCCA; CTCGGCCATGAAGTTCTCTCAGAAGGGGTGTGCGGAGATGATCATCAGCATGGACAGCAGCCAGATCCAC; AGCAAAGACCCCCGTTACGGAGCCAGCCCCCTCCACTGGGCCAAGAACGCAGAGATGGCCCGCATGCTGC; TGAAACGGGGCTGCAACGTGAACAGCACCAGCTCCGCGGGGAACACGGCCCTGCACGTGGCGGTGATGCG; CAACCGCTTCGACTGTGCCATAGTGCTGCTGACCCACGGGGCCAACGCGGATGCCCGCGGAGAGCACGGC; AACACCCCGCTGCACCTGGCCATGTCGAAAGACAACGTGGAGATGATCAAGGCCCTCATCGTGTTCGGAG; CAGAAGTGGACACCCCGAATGACTTTGGGGAGACTCCTACATTCCTAGCCTCCAAAATCGGCAGACTTGT; CACCAGGAAGGCGATCTTGACTCTGCTGAGAACCGTGGGGGCCGAATACTGCTTCCCACCCATCCACGGG; GTCCCCGCGGAGCAGGGCTCTGCAGCGCCACATCATCCCTTCTCCCTGGAAAGAGCTCAGCCCCCACCGA; TCAGCCTAAACAACCTAGAACTACAGGATCTCATGCACATCTCACGGGCCCGGAAGCCAGCGTTCATCCT; GGGCTCCATGAGGGACGAGAAGCGGACCCACGACCACCTGCTGTGCCTGGATGGAGGAGGAGTGAAAGGC; CTCATCATCATCCAGCTCCTCATCGCCATCGAGAAGGCCTCGGGTGTGGCCACCAAGGACCTGTTTGACT; GGGTGGCGGGCACCAGCACTGGAGGCATCCTGGCCCTGGCCATTCTGCACAGTAAGTCCATGGCCTACAT; GCGCGGCATGTACTTTCGCATGAAGGATGAGGTGTTCCGGGGCTCCAGGCCCTACGAGTCGGGGCCCCTG; GAGGAGTTCCTGAAGCGGGAGTTTGGGGAGCACACCAAGATGACGGACGTCAGGAAACCCAAGGTGATGC; TGACAGGGACACTGTCTGACCGGCAGCCGGCTGAACTCCACCTCTTCCGGAACTACGATGCTCCAGAAAC; TGTCCGGGAGCCTCGTTTCAACCAGAACGTTAACCTCAGGCCTCCAGCTCAGCCCTCAGACCAGCTGGTG; TGGCGGGCGGCCCGAAGCAGCGGGGCAGCTCCTACTTACTTCCGACCCAATGGGCGCTTCCTGGACGGTG; GGCTGCTGGCCAACAACCCCACGCTGGATGCCATGACCGAGATCCATGAGTACAATCAGGACCTGATCCG; CAAGGGTCAGGCCAACAAGGTGAAGAAACTCTCCATCGTTGTCTCCCTGGGGACAGGGAGGTCCCCACAA; GTGCCTGTGACCTGTGTGGATGTCTTCCGTCCCAGCAACCCCTGGGAGCTGGCCAAGACTGTTTTTGGGG; CCAAGGAACTGGGCAAGATGGTGGTGGACTGTTGCACGGATCCAGACGGGCGGGCTGTGGACCGGGCACG; GGCCTGGTGCGAGATGGTCGGCATCCAGTACTTCAGATTGAACCCCCAGCTGGGGACGGACATCATGCTG; GATGAGGTCAGTGACACAGTGCTGGTCAACGCCCTCTGGGAGACCGAGGTCTACATCTATGAGCACCGCG; AGGAGTTCCAGAAGCTCATCCAGCTGCTGCTCTCACCC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207546 representing NM_003560; Red=Cloning site Green=Tags(s); ; MQFFGRLVNTFSGVTNLFSNPFRVKEVAVADYTSSDRVREEGQLILFQNTPNRTWDCVLVNPRNSQSGFR; LFQLELEADALVNFHQYSSQLLPFYESSPQVLHTEVLQHLTDLIRNHPSWSVAHLAVELGIRECFHHSRI; ISCANCAENEEGCTPLHLACRKGDGEILVELVQYCHTQMDVTDYKGETVFHYAVQGDNSQVLQLLGRNAV; AGLNQVNNQGLTPLHLACQLGKQEMVRVLLLCNARCNIMGPNGYPIHSAMKFSQKGCAEMIISMDSSQIH; SKDPRYGASPLHWAKNAEMARMLLKRGCNVNSTSSAGNTALHVAVMRNRFDCAIVLLTHGANADARGEHG; NTPLHLAMSKDNVEMIKALIVFGAEVDTPNDFGETPTFLASKIGRLVTRKAILTLLRTVGAEYCFPPIHG; VPAEQGSAAPHHPFSLERAQPPPISLNNLELQDLMHISRARKPAFILGSMRDEKRTHDHLLCLDGGGVKG; LIIIQLLIAIEKASGVATKDLFDWVAGTSTGGILALAILHSKSMAYMRGMYFRMKDEVFRGSRPYESGPL; EEFLKREFGEHTKMTDVRKPKVMLTGTLSDRQPAELHLFRNYDAPETVREPRFNQNVNLRPPAQPSDQLV; WRAARSSGAAPTYFRPNGRFLDGGLLANNPTLDAMTEIHEYNQDLIRKGQANKVKKLSIVVSLGTGRSPQ; VPVTCVDVFRPSNPWELAKTVFGAKELGKMVVDCCTDPDGRAVDRARAWCEMVGIQYFRLNPQLGTDIML; DEVSDTVLVNALWETEVYIYEHREEFQKLIQLLLSP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003560
• ORF Size: 2418 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003560.4
• RefSeq Size: 3239 bp
• RefSeq ORF: 2421 bp
• Locus ID: 8398
• UniProt ID: O60733
• Cytogenetics: 22q13.1
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• MW: 89.7 kDa
• Summary: The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only three of them have been determined to date. [provided by RefSeq, Dec 2010]