D2HGDH (NM_152783) Human Tagged ORF Clone

CAT#: RC207367

D2HGDH (Myc-DDK-tagged)-Human D-2-hydroxyglutarate dehydrogenase (D2HGDH), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY407275) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207367 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified D2HGDH protein (Cat# TP307367). The protein was produced from HEK293T cells transfected with D2HGDH cDNA clone (Cat# RC207367) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: D2HGDH
• Synonyms: D2HGD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207367 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCCGTCGGCCTCTGGCGTGGCCCGCGTGGCTGTTGCGGGGTGCTCCGGGAGCCGCGGGTTCTT
  GGGGTCGGCCGGTTGGCCCCCTGGCCCGCAGAGGCTGCTGCTCCGCCCCGGGGACCCCCGAGGTGCCGCT
  GACCCGGGAGCGCTACCCCGTGCAGCGCTTGCCGTTCTCCACGGTGTCTAAGCAGGACCTGGCCGCCTTT
  GAGCGCATCGTGCCCGGCGGGGTCGTCACGGACCCGGAAGCGCTGCAGGCTCCCAACGTGGACTGGTTGC
  GGACGCTGCGAGGCTGTAGCAAGGTGCTGCTGAGGCCACGGACGTCGGAGGAGGTGTCCCACATCCTCAG
  GCACTGCCACGAGAGGAACCTGGCCGTGAACCCACAGGGGGGCAACACAGGCATGGTGGGTGGCAGCGTC
  CCCGTCTTTGACGAGATCATCCTCTCCACTGCCCGCATGAACCGGGTCCTCAGCTTCCACAGCGTGTCTG
  GAATTCTGGTTTGCCAGGCGGGCTGCGTCCTGGAGGAGCTGAGCCGGTATGTGGAGGAACGGGACTTCAT
  CATGCCGCTGGACTTAGGAGCCAAGGGCAGCTGCCACATCGGGGGAAACGTGGCAACCAACGCTGGAGGC
  CTGCGGTTTCTTCGATATGGCTCACTGCATGGGACTGTCCTGGGCCTGGAAGTGGTGCTGGCCGACGGCA
  CTGTCCTGGACTGCCTGACCTCCCTGAGGAAGGACAACACGGGCTATGACCTGAAGCAGCTGTTCATCGG
  GTCGGAGGGCACTTTGGGGATCATCACCACGGTGTCCATCTTGTGTCCACCCAAGCCCAGGGCTGTGAAC
  GTGGCTTTCCTCGGCTGCCCAGGCTTTGCTGAGGTTCTGCAGACCTTCAGCACCTGCAAGGGGATGCTGG
  GTGAGATCCTGTCTGCATTCGAGTTCATGGATGCTGTGTGCATGCAGCTGGTCGGGCGCCATCTCCACCT
  GGCCAGCCCGGTGCAAGAGAGTCCGTTTTACGTCCTCATCGAGACTTCAGGCTCCAACGCAGGCCATGAC
  GCTGAGAAGCTGGGCCACTTCCTGGAGCACGCGCTGGGCTCCGGCCTGGTGACCGATGGGACCATGGCCA
  CCGACCAGAGGAAAGTCAAGATGCTGTGGGCCCTGAGGGAAAGGATCACAGAGGCGCTGAGCCGGGATGG
  CTACGTGTACAAGTACGACCTCTCCCTCCCTGTGGAGCGGCTCTACGACATCGTGACTGACCTGCGCGCC
  CGCCTCGGCCCGCACGCCAAGCACGTGGTGGGCTATGGCCACCTTGGAGATGGTAACCTGCACCTCAATG
  TGACGGCGGAGGCCTTCAGCCCCTCGCTCCTGGCTGCCCTGGAGCCCCACGTGTACGAGTGGACGGCCGG
  GCAGCAGGGCAGCGTCAGCGCGGAGCACGGAGTGGGCTTCAGGAAGAGGGACGTCCTGGGCTACAGCAAG
  CCACCGGGGGCCCTGCAGCTCATGCAGCAGCTCAAGGCCCTGCTGGACCCCAAGGGCATCCTCAACCCCT
  ACAAGACGCTGCCCAGCCAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207367 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPRRPLAWPAWLLRGAPGAAGSWGRPVGPLARRGCCSAPGTPEVPLTRERYPVQRLPFSTVSKQDLAAF
  ERIVPGGVVTDPEALQAPNVDWLRTLRGCSKVLLRPRTSEEVSHILRHCHERNLAVNPQGGNTGMVGGSV
  PVFDEIILSTARMNRVLSFHSVSGILVCQAGCVLEELSRYVEERDFIMPLDLGAKGSCHIGGNVATNAGG
  LRFLRYGSLHGTVLGLEVVLADGTVLDCLTSLRKDNTGYDLKQLFIGSEGTLGIITTVSILCPPKPRAVN
  VAFLGCPGFAEVLQTFSTCKGMLGEILSAFEFMDAVCMQLVGRHLHLASPVQESPFYVLIETSGSNAGHD
  AEKLGHFLEHALGSGLVTDGTMATDQRKVKMLWALRERITEALSRDGYVYKYDLSLPVERLYDIVTDLRA
  RLGPHAKHVVGYGHLGDGNLHLNVTAEAFSPSLLAALEPHVYEWTAGQQGSVSAEHGVGFRKRDVLGYSK
  PPGALQLMQQLKALLDPKGILNPYKTLPSQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152783
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152783.2, NP_689996.3
• RefSeq Size: 2660 bp
• RefSeq ORF: 1566 bp
• Locus ID: 728294
• UniProt ID: Q8N465
• Cytogenetics: 2q37.3
• MW: 56.4 kDa
• Gene Summary: This gene encodes D-2hydroxyglutarate dehydrogenase, a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme, which is most active in liver and kidney but also active in heart and brain, converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria, a rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. [provided by RefSeq, Jul 2008]