D2HGDH (NM_152783) Human Untagged Clone

CAT#: SC309200

D2HGDH (untagged)-Human D-2-hydroxyglutarate dehydrogenase (D2HGDH), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: D2HGDH
• Synonyms: D2HGD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_152783 edited
  GGCGGCTCTGGGCCTGCGGCGGGCGCTGCGGATTGGAGCTCGGGACGTGGTCGTGGGCGC
  GCAGCCAGCGGCTCCCTGCCCTTCCCCTCCGGGCCCTGAGTACCGGCCCCCCACCAAGGA
  GGAGCCCGAGGTCTCCGTCCCGGCGGCGATGCTGCCCCGTCGGCCTCTGGCGTGGCCCGC
  GTGGCTGTTGCGGGGTGCTCCGGGAGCCGCGGGTTCTTGGGGTCGGCCGGTTGGCCCCCT
  GGCCCGCAGAGGCTGCTGCTCCGCCCCGGGGACCCCCGAGGTGCCGCTGACCCGGGAGCG
  CTACCCCGTGCAGCGCTTGCCGTTCTCCACGGTGTCTAAGCAGGACCTGGCCGCCTTTGA
  GCGCATCGTGCCCGGCGGGGTCGTCACGGACCCGGAAGCGCTGCAGGCTCCCAACGTGGA
  CTGGTTGCGGACGCTGCGAGGCTGTAGCAAGGTGCTGCTGAGGCCACGGACGTCGGAGGA
  GGTGTCCCACATCCTCAGGCACTGCCACGAGAGGAACCTGGCCGTGAACCCACAGGGGGG
  CAACACAGGCATGGTGGGTGGCAGCGTCCCCGTCTTTGACGAGATCATCCTCTCCACTGC
  CCGCATGAACCGGGTCCTCAGCTTCCACAGCGTGTCTGGAATTCTGGTTTGCCAGGCGGG
  CTGCGTCCTGGAGGAGCTGAGCCGGTATGTGGAGGAACGGGACTTCATCATGCCGCTGGA
  CTTAGGAGCCAAGGGCAGCTGCCACATCGGGGGAAACGTGGCAACCAACGCTGGAGGCCT
  GCGGTTTCTTCGATATGGCTCACTGCATGGGACTGTCCTGGGCCTGGAAGTGGTGCTGGC
  CGACGGCACTGTCCTGGACTGCCTGACCTCCCTGAGGAAGGACAACACGGGCTATGACCT
  GAAGCAGCTGTTCATCGGGTCGGAGGGCACTTTGGGGATCATCACCACGGTGTCCATCTT
  GTGTCCACCCAAGCCCAGGGCTGTGAACGTGGCTTTCCTCGGCTGCCCAGGCTTTGCTGA
  GGTTCTGCAGACCTTCAGCACCTGCAAGGGGATGCTGGGTGAGATCCTGTCTGCATTCGA
  GTTCATGGATGCTGTGTGCATGCAGCTGGTCGGGCGCCATCTCCACCTGGCCAGCCCGGT
  GCAAGAGAGTCCGTTTTACGTCCTCATCGAGACTTCAGGCTCCAACGCAGGCCATGACGC
  TGAGAAGCTGGGCCACTTCCTGGAGCACGCGCTGGGCTCCGGCCTGGTGACCGATGGGAC
  CATGGCCACCGACCAGAGGAAAGTCAAGATGCTGTGGGCCCTGAGGGAAAGGATCACAGA
  GGCGCTGAGCCGGGATGGCTACGTGTACAAGTACGACCTCTCCCTCCCTGTGGAGCGGCT
  CTACGACATCGTGACTGACCTGCGCGCCCGCCTCGGCCCGCACGCCAAGCACGTGGTGGG
  CTATGGCCACCTTGGAGATGGTAACCTGCACCTCAATGTGACGGCGGAGGCCTTCAGCCC
  CTCGCTCCTGGCTGCCCTGGAGCCCCACGTGTACGAGTGGACGGCCGGGCAGCAGGGCAG
  CGTCAGCGCGGAGCACGGAGTGGGCTTCAGGAAGAGGGACGTCCTGGGCTACAGCAAGCC
  ACCGGGGGCCCTGCAGCTCATGCAGCAGCTCAAGGCCCTGCTGGACCCCAAGGGCATCCT
  CAACCCCTACAAGACGCTGCCCAGCCAGGCCTGACGGCCACTCCTGCTGCTGCCAAGGCC
  CACTGGGGGTCGGCGGGTGGCTCTCGGGCGGGGGTGTTGCGGTGGCTCTGAGGGATGAGC
  CGGCAGTGGGCAGGGGACCAGGCACCTGGTTGAAGGGACTGGGAGCCCGCACTGGGGAAC
  TGCCGGACGCAGGCCCTCGGGCAGGAGCATCTGGCAGAGTGGGGGGCGTGGCAGGCACCC
  TCCTTTGCAGGGCGAGGTGGGGCCTCTGCAGCCATCCTGGACAGGCCGGGGTGGCGGCAG
  CTTTGCCCACGTGGAAGCGGGGTGGGTCTCACTTGCGTGGTGGCCCCTGGCCCCATCTTG
  CCTGCTGCGGCCTGGGGAGCAGGCGCTGGGTGGTGGTTCTGCCTGCTTGCTGCTCGTTCC
  CCGGGCATGCGTGGGCAGCGGGGGGCATGCGTGGGCAGCAGGGGGCGTGGGCAGCGGGGG
  CATGGGCAGGACCACGTGGGCCGTGATCGTGGGTTGCCGAGAGGACCTGAGCGCTGCGGC
  TCTGCTGAATGGAGCCGGGTCCCTCAGGCCGTGGACGCCCTCGGGAGGGGGGTGACTGTG
  GCTTGTGTCTGGACAGGAATGTGTCATTTCCCACATCTTCTAGAGGGCTGCCAGCTGGGA
  AGACAGTTATCAGGGCAAGCTGTGCTCTGAGTTTCGGGTTCTGCTCCTACAAAGAACGTG
  CGGTGCTGCGGGCGAGGGCCCCGGCACGGACAAGGGCCACTGCAGAGTGTGTTTCTGCTC
  GTCAGCTGCCCTGGGCAGCGGATGGGCTGGGCGATGCAGCTGGATGCACATCTCATTCTG
  TCATGAATGTCCAGTAAAAATCTGAATTGGTTGCAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_152783
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152783.2, NP_689996.3
• RefSeq Size: 2570 bp
• RefSeq ORF: 1566 bp
• Locus ID: 728294
• UniProt ID: Q8N465
• Cytogenetics: 2q37.3
• Gene Summary: This gene encodes D-2hydroxyglutarate dehydrogenase, a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme, which is most active in liver and kidney but also active in heart and brain, converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria, a rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1).