RNF4 (NM_002938) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RNF4 |
| Synonyms | RES4-26; SLX5; SNURF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002938 |
| ORF Size | 570 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002938.5 |
| RefSeq Size | 2971 bp |
| RefSeq ORF | 573 bp |
| Locus ID | 6047 |
| UniProt ID | P78317 |
| Cytogenetics | 4p16.3 |
| Domains | RING |
| Protein Families | Transcription Factors |
| MW | 21.3 kDa |
| Summary | The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.provided by RefSeq, Jul 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002938" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207273L1 | Lenti ORF clone of Human ring finger protein 4 (RNF4), transcript variant 2, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC207273L2 | Lenti ORF clone of Human ring finger protein 4 (RNF4), transcript variant 2, mGFP tagged | 10 ug |
$600.00
|
|
| RC207273L3 | Lenti ORF clone of Human ring finger protein 4 (RNF4), transcript variant 2, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC207273L4 | Lenti ORF clone of Human ring finger protein 4 (RNF4), transcript variant 2, mGFP tagged | 10 ug |
$600.00
|
|
| RG207273 | RNF4 (tGFP-tagged) - Human ring finger protein 4 (RNF4), transcript variant 2 | 10 ug |
$489.00
$500.00
|
|
| SC110999 | RNF4 (untagged)-Human ring finger protein 4 (RNF4), transcript variant 2 | 10 ug |
$300.00
|
|
Citations
Piperlongumine overcomes osimertinib resistance via governing ubiquitination-modulated Sp1 turnover
Wang, Wang, Liao et al
JCI Insight (2025) 10 (6) DOI: 10.1172/jci.insight.186165
Wang, Wang, Liao et al
JCI Insight (2025) 10 (6) DOI: 10.1172/jci.insight.186165
Cited by 6
AI Summary: OriGene products, specifically the Sp1 (SC101137) and RNF4 (RC207273) cDNA plasmids, were used in this study to investigate the molecular mechanisms by which piperlongumine overcomes osimertinib resistance in non-small cell lung cancer.
Snippet: Sp1 shRNA (#1, TRCN0000020444;#2, TRCN0000020445), and RNF4 shRNA (#1, TRCN0000017053;#2, TRCN0000017054), were purchased from GE Horizon. cDNA plasmids, including Sp1 (SC101137) and RNF4 (RC207273), were obtained from Origene
SPRTN patient variants cause global-genome DNA-protein crosslink repair defects
Weickert, Li, Götz et al
Nat Commun (2023) 14 (1), 352 DOI: 10.1038/s41467-023-35988-1
Weickert, Li, Götz et al
Nat Commun (2023) 14 (1), 352 DOI: 10.1038/s41467-023-35988-1
Cited by 38
AI Summary: OriGene's pCMV6-RNF4-DDK-Myc plasmid (#RC207273) was used in this study to investigate the mechanisms of DNA-protein crosslink repair involving SPRTN patient variants.
Snippet: dered significantly enriched if their log2 fold enrichment was greater than 2 and FDR adjusted p-value smaller than 0.01. Plasmids and site-directed mutagenesis pCMV6-RNF4-DDK-Myc was purchased from Origene (#RC207273). pIRES-AcFL was a gift from the Boulton lab. pcDNA5-FRT/TO-YFP-SPRTN-WT-Strep, pcDNA5-FRT/TO-YFP-SPRTN-EQ (E112Q)-Strep pcDNA5-FRT/TO-YFP-SPRTN-ΔC-Strep, pcDNA5-FRT/TO-YFP-SPRTN-P
The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin
Krastev, Li, Sun et al
Nat Cell Biol (2022) 24 (1), 62-73 DOI: 10.1038/s41556-021-00807-6
Krastev, Li, Sun et al
Nat Cell Biol (2022) 24 (1), 62-73 DOI: 10.1038/s41556-021-00807-6
Cited by 165
AI Summary: OriGene contributed the RNF4 expression construct (RC207273), which was used to study the role of RNF4 in the ubiquitylation and removal of trapped PARP1 from chromatin.
Snippet: …The wild-type PIAS4-expressing construct was obtained from Addgene (15208) and RNF4 from Origene (RC207273). … The RNF4-M136S,R177A was a gift from R. … Antibodies to the following were;The wild-type PIAS4-expressing construct was obtained from Addgene (15208) and RNF4 from Origene (RC207273)
Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications
Ward, Kleinman, Brittain et al
ACS Chem Biol (2019) 14 (11), 2430-2440 DOI: 10.1021/acschembio.8b01083
Ward, Kleinman, Brittain et al
ACS Chem Biol (2019) 14 (11), 2430-2440 DOI: 10.1021/acschembio.8b01083
Cited by 343
AI Summary: In this study, OriGene products—specifically the RNF4 Myc-DDK tagged overexpression plasmid (Cat# RC204232) and RNF4-specific siRNA (Cat# SR300634)—were used to manipulate RNF4 expression levels, enabling the authors to validate the role of RNF4 in targeted protein degradation mediated by their newly identified covalent ligand recruiter.
Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications
Ward, Kleinman, Brittain et al
bioRxiv (2018)
Ward, Kleinman, Brittain et al
bioRxiv (2018)
Trafficking of the transcription factor Nrf2 to promyelocytic leukemia-nuclear bodies: implications for degradation of NRF2 in the nucleus
Malloy, McIntosh, Walters et al
J. Biol. Chem. (2013) 288 (20), 14569-83 DOI: 10.1074/jbc.M112.437392
Malloy, McIntosh, Walters et al
J. Biol. Chem. (2013) 288 (20), 14569-83 DOI: 10.1074/jbc.M112.437392
Cited by 3
Showing 1-6 of 8 papers.
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