ATP6V1H (NM_015941) Human Tagged ORF Clone

SKU RC206841

ATP6V1H (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ATP6V1H
• Synonyms: CGI-11; MSTP042; NBP1; SFD; SFDalpha; SFDbeta; VMA13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206841 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACCAAAATGGATATCCGAGGTGCTGTGGATGCTGCTGTCCCCACCAATATTATTGCTGCCAAGGCTG; CAGAAGTTCGTGCAAACAAAGTCAACTGGCAATCCTATCTTCAGGGACAGATGATTTCTGCTGAAGATTG; TGAGTTTATTCAGAGGTTTGAAATGAAACGAAGCCCTGAAGAGAAGCAAGAGATGCTTCAAACTGAAGGC; AGCCAGTGTGCTAAAACATTTATAAATCTGATGACTCATATCTGCAAAGAACAGACCGTTCAGTATATAC; TAACTATGGTGGATGATATGCTGCAGGAAAATCATCAGCGTGTTAGCATTTTCTTTGACTATGCAAGATG; TAGCAAGAACACTGCGTGGCCCTACTTTCTGCCAATGTTGAATCGCCAGGATCCCTTCACTGTTCATATG; GCAGCAAGAATTATTGCCAAGTTAGCAGCTTGGGGAAAAGAACTGATGGAAGGCAGTGACTTAAATTACT; ATTTCAATTGGATAAAAACTCAGCTGAGTTCACAGAAACTGCGTGGTAGCGGTGTTGCTGTTGAAACAGG; AACAGTCTCTTCAAGTGATAGTTCGCAGTATGTGCAGTGCGTGGCCGGGTGTTTGCAGCTGATGCTCCGG; GTCAATGAGTACCGCTTTGCTTGGGTGGAAGCAGATGGGGTAAATTGCATAATGGGAGTGTTGAGTAACA; AGTGTGGCTTTCAGCTCCAGTATCAAATGATTTTTTCAATATGGCTCCTGGCATTCAGTCCTCAAATGTG; TGAACACCTGCGGCGCTATAATATCATTCCAGTTCTGTCTGATATCCTTCAGGAGTCTGTCAAAGAGAAA; GTAACAAGAATCATTCTTGCAGCATTTCGTAACTTTTTAGAAAAATCAACTGAAAGAGAAACTCGCCAAG; AATATGCCCTGGCTATGATTCAGTGCAAAGTTCTGAAACAGTTGGAGAACTTGGAACAGCAGAAGTACGA; TGATGAAGATATCAGCGAAGATATCAAATTTCTTTTGGAAAAACTTGGAGAGAGTGTCCAGGACCTTAGT; TCATTTGATGAATACAGTTCAGAACTTAAATCTGGAAGGTTGGAATGGAGTCCTGTGCACAAATCTGAGA; AATTTTGGAGAGAGAATGCTGTGAGGTTAAATGAGAAGAATTATGAACTCTTGAAAATCTTGACAAAACT; TTTGGAAGTGTCAGATGATCCCCAAGTCTTAGCTGTTGCTGCTCACGATGTTGGAGAATATGTGCGGCAT; TATCCACGAGGCAAACGGGTCATCGAGCAGCTCGGTGGGAAGCAGCTGGTCATGAACCACATGCATCATG; AAGACCAGCAGGTCCGCTATAATGCTCTGCTGGCCGTGCAGAAGCTCATGGTGCACAACTGGGAATACCT; TGGCAAGCAGCTCCAGTCCGAGCAGCCCCAGACCGCTGCCGCCCGAAGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206841 protein sequence; Red=Cloning site Green=Tags(s); ; MTKMDIRGAVDAAVPTNIIAAKAAEVRANKVNWQSYLQGQMISAEDCEFIQRFEMKRSPEEKQEMLQTEG; SQCAKTFINLMTHICKEQTVQYILTMVDDMLQENHQRVSIFFDYARCSKNTAWPYFLPMLNRQDPFTVHM; AARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGSGVAVETGTVSSSDSSQYVQCVAGCLQLMLR; VNEYRFAWVEADGVNCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEK; VTRIILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLS; SFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRH; YPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015941
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015941.4
• RefSeq Size: 2186 bp
• RefSeq ORF: 1452 bp
• Locus ID: 51606
• UniProt ID: Q9UI12
• Cytogenetics: 8q11.23
• Domains: V-ATPase_H
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• MW: 55.9 kDa
• Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]