PHLDA3 (NM_012396) Human Tagged ORF Clone
SKU
RC206751
PHLDA3 (Myc-DDK-tagged)-Human pleckstrin homology-like domain, family A, member 3 (PHLDA3)
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PHLDA3 |
| Synonyms | TIH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012396 |
| ORF Size | 381 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012396.5 |
| RefSeq Size | 1516 bp |
| RefSeq ORF | 384 bp |
| Locus ID | 23612 |
| UniProt ID | Q9Y5J5 |
| Cytogenetics | 1q32.1 |
| Domains | PH |
| MW | 13.9 kDa |
| Summary | p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012396" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206751L1 | Lenti ORF clone of Human pleckstrin homology-like domain, family A, member 3 (PHLDA3), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC206751L2 | Lenti ORF clone of Human pleckstrin homology-like domain, family A, member 3 (PHLDA3), mGFP tagged | 10 ug |
$450.00
|
|
| RC206751L3 | Lenti ORF clone of Human pleckstrin homology-like domain, family A, member 3 (PHLDA3), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC206751L4 | Lenti ORF clone of Human pleckstrin homology-like domain, family A, member 3 (PHLDA3), mGFP tagged | 10 ug |
$450.00
|
|
| RG206751 | PHLDA3 (tGFP-tagged) - Human pleckstrin homology-like domain, family A, member 3 (PHLDA3) | 10 ug |
$489.00
|
|
| SC115414 | PHLDA3 (untagged)-Human pleckstrin homology-like domain, family A, member 3 (PHLDA3) | 10 ug |
$150.00
|
|
Citations
PHLDA3 promotes lung adenocarcinoma cell proliferation and invasion via activation of the Wnt signaling pathway
Lei, Wang, Li et al
Lab Invest (2021)
Lei, Wang, Li et al
Lab Invest (2021)
Cited by 23
AI Summary: OriGene products pCMV6-Myc-DDK (#PS100001) and pCMV6-Myc-DDK-PHLDA3 (#RC206751) were used to manipulate PHLDA3 expression in lung adenocarcinoma cells, enabling the study of its role in cell proliferation and invasion.
Snippet: pCMV6-Myc-DDK (#PS100001) and pCMV6-Myc-DDK-PHLDA3 (#RC206751) were purchased from Origene (Rockville, MD, USA). siRNA against PHLDA3 (siPHLDA3) and control scrambled siRNA were purchased from RiboBio (Guangzhou, China);pCMV6-Myc-DDK (#PS100001) and pCMV6-Myc-DDK-PHLDA3 (#RC206751) were purchased from Origene (Rockville, MD, USA)
Usefulness of an immunohistochemical score in advanced pancreatic neuroendocrine tumors treated with CAPTEM or everolimus
Viúdez, Crespo, Gómez Dorronsoro et al
Pancreatology (2020) 21 (1), 215-223 DOI: 10.1016/j.pan.2020.12.009
Viúdez, Crespo, Gómez Dorronsoro et al
Pancreatology (2020) 21 (1), 215-223 DOI: 10.1016/j.pan.2020.12.009
Cited by 4
AI Summary: OriGene contributed to this study by providing pCMV6 vectors for NDRG-1 and PHLDA-3 cDNA overexpression, which were used to generate cell lines for functional analysis of these genes in pancreatic neuroendocrine tumors.
Snippet: pCMV6 vectors for NDRG-1 or PHLDA-3 cDNA overexpression were purchased from Origene. Cells were transfected with the corresponding cDNA or an empty vector as a control, and selected with hygromycin until established clones were selected. Colony formation
Showing 1-2 of 2 papers.
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