HSD17B3 (NM_000197) Human Tagged ORF Clone

CAT#: RC206558

HSD17B3 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HSD17B3 (Cat# RC206558, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B3 (Cat# TA811500)(1:2000). Positive lysates LY424871 (100ug) and LC424871 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY424871) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206558 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HSD17B3 protein (Cat# TP306558). The protein was produced from HEK293T cells transfected with HSD17B3 cDNA clone (Cat# RC206558) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HSD17B3
• Synonyms: EDH17B3; SDR12C2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206558 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACGTCCTGGAACAGTTCTTCATCCTCACAGGGCTGCTGGTGTGCCTGGCCTGCCTGGCGAAGT
  GCGTGAGATTCTCCAGATGTGTTTTACTGAACTACTGGAAAGTTTTGCCAAAGTCTTTCTTGCGGTCAAT
  GGGACAGTGGGCAGTGATCACTGGAGCAGGCGATGGAATTGGGAAAGCGTACTCGTTCGAGCTAGCAAAA
  CGTGGACTCAATGTTGTCCTTATTAGCCGGACGCTGGAAAAACTAGAGGCCATTGCCACAGAGATCGAGC
  GGACTACAGGGAGGAGTGTGAAGATTATACAAGCAGATTTTACAAAAGATGACATCTACGAGCATATTAA
  AGAAAAACTTGCAGGCTTAGAAATTGGAATTTTAGTCAACAATGTCGGAATGCTTCCAAACCTTCTCCCA
  AGCCATTTCCTGAACGCACCGGATGAAATCCAGAGCCTCATCCATTGTAACATCACCTCCGTAGTCAAGA
  TGACACAGCTAATTCTGAAACATATGGAATCAAGGCAGAAAGGTCTCATCCTGAACATTTCTTCTGGGAT
  AGCCCTGTTTCCTTGGCCTCTCTACTCCATGTACTCAGCTTCCAAGGCGTTTGTGTGCGCATTTTCCAAG
  GCCCTGCAAGAGGAATATAAAGCAAAAGAAGTCATCATCCAGGTGCTGACCCCATATGCTGTCTCGACTG
  CAATGACAAAGTATCTAAATACAAATGTGATAACCAAGACTGCTGATGAGTTTGTCAAAGAGTCATTGAA
  TTATGTCACAATTGGAGGTGAAACCTGTGGCTGCCTTGCCCATGAAATCTTGGCGGGCTTTCTGAGCCTG
  ATCCCGGCCTGGGCCTTCTACAGCGGTGCCTTCCAAAGGCTGCTCCTGACACACTATGTGGCATACCTGA
  AGCTCAACACCAAGGTCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206558 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDVLEQFFILTGLLVCLACLAKCVRFSRCVLLNYWKVLPKSFLRSMGQWAVITGAGDGIGKAYSFELAK
  RGLNVVLISRTLEKLEAIATEIERTTGRSVKIIQADFTKDDIYEHIKEKLAGLEIGILVNNVGMLPNLLP
  SHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQKGLILNISSGIALFPWPLYSMYSASKAFVCAFSK
  ALQEEYKAKEVIIQVLTPYAVSTAMTKYLNTNVITKTADEFVKESLNYVTIGGETCGCLAHEILAGFLSL
  IPAWAFYSGAFQRLLLTHYVAYLKLNTKVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000197
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000197.2
• RefSeq Size: 1134 bp
• RefSeq ORF: 933 bp
• Locus ID: 3293
• UniProt ID: P37058
• Cytogenetics: 9q22.32
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• MW: 34.5 kDa
• Gene Summary: This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]