HSD17B3 (NM_000197) Human Untagged Clone

CAT#: SC300022

HSD17B3 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 3 (HSD17B3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD17B3
• Synonyms: EDH17B3; SDR12C2
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000197 edited
  GCCAGGGCTGAAACAGTCTGTTGAGTGCAGCCATGGGGGACGTCCTGGAACAGTTCTTCA
  TCCTCACAGGGCTGCTGGTGTGCCTGGCCTGCCTGGCGAAGTGCGTGAGATTCTCCAGAT
  GTGTTTTACTGAACTACTGGAAAGTTTTGCCAAAGTCTTTCTTGCGGTCAATGGGACAGT
  GGGCAGTGATCACTGGAGCAGGCGATGGAATTGGGAAAGCGTACTCGTTCGAGCTAGCAA
  AACGTGGACTCAATGTTGTCCTTATTAGCCGGACGCTGGAAAAACTAGAGGCCATTGCCA
  CAGAGATCGAGCGGACTACAGGGAGGAGTGTGAAGATTATACAAGCAGATTTTACAAAAG
  ATGACATCTACGAGCATATTAAAGAAAAACTTGCAGGCTTAGAAATTGGAATTTTAGTCA
  ACAATGTCGGAATGCTTCCAAACCTTCTCCCAAGCCATTTCCTGAACGCACCGGATGAAA
  TCCAGAGCCTCATCCATTGTAACATCACCTCCGTAGTCAAGATGACACAGCTAATTCTGA
  AACATATGGAATCAAGGCAGAAAGGTCTCATCCTGAACATTTCTTCTGGGATAGCCCTGT
  TTCCTTGGCCTCTCTACTCCATGTACTCAGCTTCCAAGGCGTTTGTGTGCGCATTTTCCA
  AGGCCCTGCAAGAGGAATATAAAGCAAAAGAAGTCATCATCCAGGTGCTGACCCCATATG
  CTGTCTCGACTGCAATGACAAAGTATCTAAATACAAATGTGATAACCAAGACTGCTGATG
  AGTTTGTCAAAGAGTCATTGAATTATGTCACAATTGGAGGTGAAACCTGTGGCTGCCTTG
  CCCATGAAATCTTGGCGGGCTTTCTGAGCCTGATCCCGGCCTGGGCCTTCTACAGCGGTG
  CCTTCCAAAGGCTGCTCCTGACACACTATGTGGCATACCTGAAGCTCAACACCAAGGTCA
  GGTAGCCAGGCGGTGAGGAGTCCAGCACAACCTTTTCCTCACCAGTCCCATGCTGGCTGA
  AGAGGACCAGAGGAGCAGACCAGCACTTCAACCTAGTCCGCTGAAGATGGAGGGGGCTGG
  GGTCACAGAGGCATAGAATACACATTTTTTGCCACTTTAAAAAAAAAAAAAAAAAAAAAA
  AAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000197
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000197.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000197.1, NP_000188.1
• RefSeq Size: 1134 bp
• RefSeq ORF: 933 bp
• Locus ID: 3293
• UniProt ID: P37058
• Cytogenetics: 9q22.32
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• Gene Summary: This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]