ENPP6 (NM_153343) Human Tagged ORF Clone

SKU RC206360

ENPP6 (Myc-DDK-tagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 6 (ENPP6)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ENPP6
• Synonyms: NPP6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206360 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGTGAAGCTTGGGACCCTCCTGCTGGCCCTTGCCCTGGGCCTGGCCCAGCCAGCCTCTGCCCGCC; GGAAGCTGCTGGTGTTTCTGCTGGATGGTTTTCGCTCAGACTACATCAGTGATGAGGCGCTGGAGTCATT; GCCTGGTTTCAAAGAGATTGTGAGCAGGGGAGTAAAAGTGGATTACTTGACTCCAGACTTCCCTAGTCTC; TCGTATCCCAATTATTATACCCTAATGACTGGCCGCCATTGTGAAGTCCATCAGATGATCGGGAACTACA; TGTGGGACCCCACCACCAACAAGTCCTTTGACATTGGCGTCAACAAAGACAGCCTAATGCCTCTCTGGTG; GAATGGATCAGAACCTCTGTGGGTCACTCTGACCAAGGCCAAAAGGAAGGTCTACATGTACTACTGGCCA; GGCTGTGAGGTTGAGATTCTGGGTGTCAGACCCACCTACTGCCTAGAATATAAAAATGTCCCAACGGATA; TCAATTTTGCCAATGCAGTCAGCGATGCTCTTGACTCCTTCAAGAGTGGCCGGGCCGACCTGGCAGCCAT; ATACCATGAGCGCATTGACGTGGAAGGCCACCACTACGGGCCTGCATCTCCGCAGAGGAAAGATGCCCTC; AAGGCTGTAGACACTGTCCTGAAGTACATGACCAAGTGGATCCAGGAGCGGGGCCTGCAGGACCGCCTGA; ACGTCATTATTTTCTCGGATCACGGAATGACCGACATTTTCTGGATGGACAAAGTGATTGAGCTGAATAA; GTACATCAGCCTGAATGACCTGCAGCAAGTGAAGGACCGCGGGCCTGTTGTGAGCCTTTGGCCGGCCCCT; GGGAAACACTCTGAGATATATAACAAACTGAGCACAGTGGAACACATGACTGTCTACGAGAAAGAAGCCA; TCCCAAGCAGGTTCTATTACAAGAAAGGAAAGTTTGTCTCTCCTTTGACTTTAGTGGCTGATGAAGGCTG; GTTCATAACTGAGAATCGAGAGATGCTTCCGTTTTGGATGAACAGCACCGGCAGGCGGGAAGGTTGGCAG; CGTGGATGGCACGGCTACGACAACGAGCTCATGGACATGCGGGGCATCTTCCTGGCCTTCGGACCTGATT; TCAAATCCAACTTCAGAGCTGCTCCTATCAGGTCGGTGGACGTCTACAATGTCATGTGCAATGTGGTGGG; CATCACCCCGCTGCCCAACAACGGATCCTGGTCCAGGGTGATGTGCATGCTGAAGGGCCGCGCCAGCACT; GCCCCGCCTGTCTGGCCCAGCCACTGTGCCCTGGCACTGATTCTTCTCTTCCTGCTTGCA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206360 protein sequence; Red=Cloning site Green=Tags(s); ; MAVKLGTLLLALALGLAQPASARRKLLVFLLDGFRSDYISDEALESLPGFKEIVSRGVKVDYLTPDFPSL; SYPNYYTLMTGRHCEVHQMIGNYMWDPTTNKSFDIGVNKDSLMPLWWNGSEPLWVTLTKAKRKVYMYYWP; GCEVEILGVRPTYCLEYKNVPTDINFANAVSDALDSFKSGRADLAAIYHERIDVEGHHYGPASPQRKDAL; KAVDTVLKYMTKWIQERGLQDRLNVIIFSDHGMTDIFWMDKVIELNKYISLNDLQQVKDRGPVVSLWPAP; GKHSEIYNKLSTVEHMTVYEKEAIPSRFYYKKGKFVSPLTLVADEGWFITENREMLPFWMNSTGRREGWQ; RGWHGYDNELMDMRGIFLAFGPDFKSNFRAAPIRSVDVYNVMCNVVGITPLPNNGSWSRVMCMLKGRAST; APPVWPSHCALALILLFLLA; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153343
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_153343.4
• RefSeq Size: 3936 bp
• RefSeq ORF: 1323 bp
• Locus ID: 133121
• UniProt ID: Q6UWR7
• Cytogenetics: 4q35.1
• Domains: Phosphodiest
• Protein Families: Secreted Protein
• Protein Pathways: Ether lipid metabolism
• MW: 50.2 kDa
• Summary: Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid.[UniProtKB/Swiss-Prot Function]