Silencer of Death Domain (BAG4) (NM_004874) Human Tagged ORF Clone

SKU RC206235

BAG4 (Myc-DDK-tagged)-Human BCL2-associated athanogene 4 (BAG4), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Silencer of Death Domain
• Synonyms: BAG-4; SODD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206235 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCGGCCCTGAGGCGCTCGGGCTACGGCCCCAGTGACGGTCCGTCCTACGGCCGCTACTACGGGCCTG; GGGGTGGAGATGTGCCGGTACACCCACCTCCACCCTTATATCCTCTTCGCCCTGAACCTCCCCAGCCTCC; CATTTCCTGGCGGGTGCGCGGGGGCGGCCCGGCGGAGACCACCTGGCTGGGAGAAGGCGGAGGAGGCGAT; GGCTACTATCCCTCGGGAGGCGCCTGGCCAGAGCCTGGTCGAGCCGGAGGAAGCCACCAGGAGCAGCCAC; CATATCCTAGCTACAATTCTAACTATTGGAATTCTACTGCGAGATCTAGGGCTCCTTACCCAAGTACATA; TCCTGTAAGACCAGAATTGCAAGGCCAGAGTTTGAATTCTTATACAAATGGAGCGTATGGTCCAACATAC; CCCCCAGGCCCTGGGGCAAATACTGCCTCATACTCAGGGGCTTATTATGCACCTGGTTATACTCAGACCA; GTTACTCCACAGAAGTTCCGAGTACTTACCGTTCATCTGGCAACAGCCCAACTCCAGTCTCTCGTTGGAT; CTATCCCCAGCAGGACTGTCAGACTGAAGCACCCCCTCTTAGGGGGCAGGTTCCAGGATATCCGCCTTCA; CAGAACCCTGGAATGACCCTGCCCCATTATCCTTATGGAGATGGTAATCGTAGTGTTCCACAATCAGGAC; CGACTGTACGACCACAAGAAGATGCGTGGGCTTCTCCTGGTGCTTATGGAATGGGTGGCCGTTATCCCTG; GCCTTCATCAGCGCCCTCAGCACCACCCGGCAATCTCTACATGACTGAAAGTACTTCACCATGGCCTAGC; AGTGGCTCTCCCCAGTCACCCCCTTCACCCCCAGTCCAGCAGCCCAAGGATTCTTCATACCCCTATAGCC; AATCAGATCAAAGCATGAACCGGCACAACTTTCCTTGCAGTGTCCATCAGTACGAATCCTCGGGGACAGT; GAACAATGATGATTCAGATCTTTTGGATTCCCAAGTCCAGTATAGTGCTGAGCCTCAGCTGTATGGTAAT; GCCACCAGTGACCATCCCAACAATCAAGATCAAAGTAGCAGTCTTCCTGAAGAATGTGTACCTTCAGATG; AAAGTACTCCTCCGAGTATTAAAAAAATCATACATGTGCTGGAGAAGGTCCAGTATCTTGAACAAGAAGT; AGAAGAATTTGTAGGAAAAAAGACAGACAAAGCATACTGGCTTCTGGAAGAAATGCTAACCAAGGAACTT; TTGGAACTGGATTCAGTTGAAACTGGGGGCCAGGACTCTGTACGGCAGGCCAGAAAAGAGGCTGTTTGTA; AGATTCAGGCCATACTGGAAAAATTAGAAAAAAAAGGATTA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206235 protein sequence; Red=Cloning site Green=Tags(s); ; MSALRRSGYGPSDGPSYGRYYGPGGGDVPVHPPPPLYPLRPEPPQPPISWRVRGGGPAETTWLGEGGGGD; GYYPSGGAWPEPGRAGGSHQEQPPYPSYNSNYWNSTARSRAPYPSTYPVRPELQGQSLNSYTNGAYGPTY; PPGPGANTASYSGAYYAPGYTQTSYSTEVPSTYRSSGNSPTPVSRWIYPQQDCQTEAPPLRGQVPGYPPS; QNPGMTLPHYPYGDGNRSVPQSGPTVRPQEDAWASPGAYGMGGRYPWPSSAPSAPPGNLYMTESTSPWPS; SGSPQSPPSPPVQQPKDSSYPYSQSDQSMNRHNFPCSVHQYESSGTVNNDDSDLLDSQVQYSAEPQLYGN; ATSDHPNNQDQSSSLPEECVPSDESTPPSIKKIIHVLEKVQYLEQEVEEFVGKKTDKAYWLLEEMLTKEL; LELDSVETGGQDSVRQARKEAVCKIQAILEKLEKKGL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004874
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004874.4
• RefSeq Size: 4478 bp
• RefSeq ORF: 1374 bp
• Locus ID: 9530
• UniProt ID: O95429
• Cytogenetics: 8p11.23
• Domains: BAG
• Protein Families: Druggable Genome
• MW: 49.6 kDa
• Summary: The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]