MTAP (NM_002451) Human Tagged ORF Clone

SKU RC206024

MTAP (Myc-DDK-tagged)-Human methylthioadenosine phosphorylase (MTAP)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MTAP
• Synonyms: BDMF; c86fus; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206024 representing NM_002451.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTGGATGATCCA; GAAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAGCCATCTGATGCCTTAATT; TTGGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAAGGCATGGAAGGCAGCACACCATCATGCCT; TCAAAGGTCAACTACCAGGCGAACATCTGGGCTTTGAAGGAAGAGGGCTGTACACATGTCATAGTGACC; ACAGCTTGTGGCTCCTTGAGGGAGGAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGAC; AGGACCACTATGAGACCTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATT; CCAATGGCTGAGCCGTTTTGCCCAAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAGGACTC; CGGTGCCACTCAAAGGGGACAATGGTCACAATCGAGGGACCTCGTTTTAGCTCCCGGGCAGAAAGCTTC; ATGTTCCGTACCTGGGGGGCGGATGTTATCAACATGACCACAGTTCCAGAGGTGGTTCTTGCTAAGGAG; GCTGGAATTTGTTACGCAAGTATCGCCATGGCGACAGATTATGACTGCTGGAAGGAGCACGAGGAAGCA; GTTTCGGTGGACCGGGTCTTAAAGACCCTGAAAGAAAACGCTAATAAAGCCAAAAGCTTACTGCTCACT; ACCATACCTCAGATAGGGTCCACAGAATGGTCAGAAACCCTCCATAACCTGAAGAATATGGCCCAGTTT; TCTGTTTTTTTACCAAGACAT; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
  Protein Sequence

  >Peptide sequence encoded by RC206024; Blue=ORF Red=Cloning site Green=Tag(s); ; MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCILLARHGRQHTIMP; SKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDRTTMRPQSFYDGSHSCARGVCHI; PMAEPFCPKTREVLIETAKKLGLRCHSKGTMVTIEGPRFSSRAESFMFRTWGADVINMTTVPEVVLAKE; AGICYASIAMATDYDCWKEHEEAVSVDRVLKTLKENANKAKSLLLTTIPQIGSTEWSETLHNLKNMAQF; SVFLPRH; TRTRPLEQKLISEEDLAANDILDYKDDDDKV; ; Recombinant protein using RC206024 also available, TP306024
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002451
• ORF Size: 849 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq Size: 4937 bp
• RefSeq ORF: 852 bp
• Locus ID: 4507
• UniProt ID: Q13126
• Cytogenetics: 9p21.3
• Domains: Mtap_PNP
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• MW: 31.3 kDa
• Summary: This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]