KDEL Receptor (KDELR1) (NM_006801) Human Tagged ORF Clone

SKU RC205880

KDELR1 (Myc-DDK-tagged)-Human KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1 (KDELR1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: KDEL Receptor
• Synonyms: ERD2; ERD2.1; HDEL; PM23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205880 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAATCTCTTCCGATTCCTGGGAGACCTCTCCCACCTCCTCGCCATCATCTTGCTACTGCTCAAAATCT; GGAAGTCCCGCTCGTGCGCCGGAATTTCAGGGAAGAGCCAGGTCCTGTTTGCTGTGGTGTTCACTGCCCG; ATATCTGGACCTCTTCACCAACTACATCTCACTCTACAACACGTGTATGAAGGTGGTCTACATAGCCTGC; TCCTTCACCACGGTCTGGTTGATTTATAGCAAGTTCAAAGCTACTTACGATGGGAACCATGACACGTTCA; GAGTGGAGTTCCTGGTCGTTCCCACAGCCATTCTGGCGTTCCTGGTCAATCATGACTTCACCCCTCTGGA; GATCCTCTGGACCTTCTCCATCTACCTGGAGTCAGTGGCCATCTTGCCGCAGCTGTTCATGGTGAGCAAG; ACCGGCGAGGCGGAGACCATCACCAGCCACTACTTGTTTGCGCTAGGCGTTTACCGCACGCTCTATCTCT; TCAACTGGATCTGGCGCTACCATTTCGAGGGCTTCTTCGACCTCATCGCCATTGTGGCAGGCCTGGTCCA; GACAGTCCTCTACTGCGATTTCTTCTACCTCTATATCACCAAAGTCCTAAAGGGGAAGAAGTTGAGTTTG; CCGGCA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205880 protein sequence; Red=Cloning site Green=Tags(s); ; MNLFRFLGDLSHLLAIILLLLKIWKSRSCAGISGKSQVLFAVVFTARYLDLFTNYISLYNTCMKVVYIAC; SFTTVWLIYSKFKATYDGNHDTFRVEFLVVPTAILAFLVNHDFTPLEILWTFSIYLESVAILPQLFMVSK; TGEAETITSHYLFALGVYRTLYLFNWIWRYHFEGFFDLIAIVAGLVQTVLYCDFFYLYITKVLKGKKLSL; PA; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006801
• ORF Size: 636 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006801.3
• RefSeq Size: 1575 bp
• RefSeq ORF: 639 bp
• Locus ID: 10945
• UniProt ID: P24390
• Cytogenetics: 19q13.33
• Domains: ER_lumen_recept
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection
• MW: 24.5 kDa
• Summary: Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq, Jul 2008]