Kindlin 2 (FERMT2) (NM_006832) Human Tagged Lenti ORF Clone

SKU
RC205727L3
Lenti ORF clone of Human fermitin family member 2 (FERMT2), transcript variant 1, Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

    Click here to learn more.

$923.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Kindlin 2
Synonyms KIND2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC205727).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_006832
ORF Size 2040 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006832.1, NP_006823.1
RefSeq Size 3351 bp
RefSeq ORF 2043 bp
Locus ID 10979
UniProt ID Q96AC1
Cytogenetics 14q22.1
MW 77.9 kDa
Summary Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC205727 FERMT2 (Myc-DDK-tagged)-Human fermitin family member 2 (FERMT2), transcript variant 1 10 ug
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RC205727L2 Lenti ORF clone of Human fermitin family member 2 (FERMT2), transcript variant 1, mGFP tagged 10 ug
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SC127300 FERMT2 (untagged)-Human fermitin family member 2 (FERMT2), transcript variant 1 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.