NDUFS1 (NM_005006) Human Tagged ORF Clone

SKU RC205506

NDUFS1 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) (NDUFS1), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: NDUFS1
• Synonyms: CI-75k; CI-75Kd; MC1DN5; PRO1304
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205506 representing NM_005006; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTAAGGATACCTGTAAGAAAGGCCTTAGTAGGCCTTTCTAAGTCTCCTAAAGGATGTGTTCGAACAA; CTGCCACAGCAGCAAGCAACTTGATTGAAGTATTTGTTGATGGTCAGTCTGTCATGGTGGAACCGGGAAC; GACCGTCCTCCAAGCTTGTGAGAAGGTTGGCATGCAGATCCCTCGATTCTGTTATCATGAAAGGTTGTCT; GTTGCTGGAAACTGCAGGATGTGCCTTGTTGAAATTGAGAAAGCCCCTAAGGTTGTAGCTGCTTGTGCCA; TGCCAGTAATGAAGGGTTGGAATATCCTAACAAACTCAGAAAAATCCAAAAAAGCCAGGGAAGGTGTGAT; GGAGTTCTTATTAGCAAATCACCCATTGGACTGTCCTATTTGTGACCAGGGAGGTGAATGTGATCTGCAG; GACCAGTCCATGATGTTTGGAAATGATAGGAGCCGATTTTTAGAGGGGAAGCGTGCTGTGGAAGACAAGA; ACATTGGGCCATTGGTAAAGACCATCATGACAAGATGTATACAGTGTACTCGCTGCATCAGGTTTGCAAG; TGAGATTGCAGGAGTAGATGATTTGGGAACAACAGGCAGAGGAAATGATATGCAAGTTGGCACATACATT; GAAAAGATGTTCATGTCTGAACTGTCTGGGAATATCATTGATATCTGCCCTGTAGGTGCCCTAACCTCTA; AGCCCTATGCCTTTACTGCCCAGCCTTGGGAAACAAGAAAGACAGAATCCATTGATGTAATGGATGCGGT; TGGAAGTAATATTGTGGTTAGCACAAGAACTGGAGAAGTGATGAGGATTTTGCCACGTATGCATGAGGAC; ATCAATGAAGAGTGGATCTCTGATAAAACCAGATTTGCCTATGATGGGCTAAAACGTCAAAGACTTACCG; AGCCAATGGTCAGAAATGAAAAAGGGCTTTTAACCTATACTTCTTGGGAGGATGCGCTCTCTCGCGTAGC; TGGAATGTTGCAGAGTTTTCAAGGCAAAGATGTGGCAGCAATTGCAGGTGGCTTGGTGGATGCTGAAGCC; CTGGTAGCTCTCAAAGATTTGCTTAATAGAGTGGACTCTGACACCTTATGCACTGAAGAGGTCTTCCCCA; CTGCAGGAGCTGGCACAGATTTGCGTTCCAATTATCTTCTTAATACTACAATTGCTGGTGTGGAAGAGGC; AGATGTTGTTCTTCTGGTTGGTACAAACCCACGTTTTGAGGCACCACTGTTTAATGCTAGAATTCGAAAG; AGCTGGCTGCATAATGACTTAAAAGTGGCCCTTATAGGCAGTCCAGTGGACCTCACTTACACATATGACC; ACCTGGGAGACTCCCCCAAAATTCTTCAAGACATTGCTTCGGGAAGCCATCCATTTAGCCAGGTCCTAAA; GGAAGCTAAAAAACCAATGGTGGTTTTAGGCAGTTCTGCACTCCAAAGAAATGATGGAGCAGCAATTCTT; GCAGCTGTTTCTAGCATTGCACAAAAGATTCGGATGACTAGTGGTGTTACTGGTGATTGGAAAGTTATGA; ATATCCTTCATAGGATTGCAAGTCAAGTAGCTGCTTTGGACCTTGGCTATAAGCCTGGGGTGGAAGCAAT; TCGGAAGAACCCTCCCAAGGTGCTGTTTCTCCTGGGAGCAGATGGAGGTTGTATCACACGACAGGATTTG; CCAAAGGATTGTTTCATTATTTATCAAGGACATCATGGTGATGTTGGGGCTCCCATAGCTGATGTTATTC; TCCCAGGAGCTGCTTACACAGAGAAGTCTGCTACATATGTCAACACTGAGGGTAGAGCTCAGCAGACTAA; GGTAGCAGTGACACCTCCTGGCTTGGCAAGAGAAGACTGGAAAATTATAAGAGCACTCTCTGAGATTGCT; GGAATGACTCTTCCATATGATACTCTGGATCAAGTAAGGAACAGATTGGAAGAAGTCTCTCCTAATCTTG; TTCGATATGATGATATTGAAGGGGCTAATTACTTCCAGCAAGCAAATGAGCTCTCAAAGCTAGTGAACCA; GCAGCTTCTTGCTGACCCACTTGTTCCACCTCAGCTAACTATAAAAGACTTCTACATGACAGATTCAATT; AGCAGAGCCTCACAGACAATGGCCAAATGTGTCAAAGCTGTCACAGAGGGTGCCCAGGCAGTAGAGGAAC; CATCCATATGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205506 representing NM_005006; Red=Cloning site Green=Tags(s); ; MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLS; VAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQ; DQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYI; EKMFMSELSGNIIDICPVGALTSKPYAFTAQPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHED; INEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTYTSWEDALSRVAGMLQSFQGKDVAAIAGGLVDAEA; LVALKDLLNRVDSDTLCTEEVFPTAGAGTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRK; SWLHNDLKVALIGSPVDLTYTYDHLGDSPKILQDIASGSHPFSQVLKEAKKPMVVLGSSALQRNDGAAIL; AAVSSIAQKIRMTSGVTGDWKVMNILHRIASQVAALDLGYKPGVEAIRKNPPKVLFLLGADGGCITRQDL; PKDCFIIYQGHHGDVGAPIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSEIA; GMTLPYDTLDQVRNRLEEVSPNLVRYDDIEGANYFQQANELSKLVNQQLLADPLVPPQLTIKDFYMTDSI; SRASQTMAKCVKAVTEGAQAVEEPSIC; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005006
• ORF Size: 2181 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005006.4
• RefSeq Size: 3417 bp
• RefSeq ORF: 2184 bp
• Locus ID: 4719
• UniProt ID: P28331
• Cytogenetics: 2q33.3
• Domains: fer2, molybdopterin
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• MW: 79.44 kDa
• Summary: The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]