KHDRBS2 (NM_152688) Human Tagged ORF Clone

SKU RC205428

KHDRBS2 (Myc-DDK-tagged)-Human KH domain containing, RNA binding, signal transduction associated 2 (KHDRBS2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: KHDRBS2
• Synonyms: SLM-1; SLM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205428 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAGAGGAGAAATATTTGCCTGAGCTGATGGCAGAGAAAGATAGCCTGGATCCATCTTTTGTGCATG; CGTCGCGCCTTTTGGCAGAAGAAATTGAAAAGTTTCAAGGTTCTGATGGAAAAAAGGAAGACGAAGAAAA; GAAGTATCTTGATGTCATCAGCAACAAAAACATAAAGCTCTCAGAAAGAGTACTGATTCCTGTCAAGCAG; TATCCAAAGTTCAATTTTGTGGGGAAATTGCTTGGACCAAGAGGAAACTCCTTGAAGAGGCTACAGGAAG; AAACAGGTGCTAAAATGTCTATCCTGGGCAAAGGATCAATGAGAGATAAAGCTAAGGAAGAAGAACTAAG; GAAGAGTGGGGAAGCCAAATATGCCCACTTGAGTGATGAGCTTCATGTATTAATTGAAGTGTTTGCTCCA; CCTGGGGAAGCTTATTCACGTATGAGTCATGCATTGGAAGAGATTAAAAAATTCCTGGTTCCTGACTACA; ATGATGAAATTCGTCAGGAACAACTACGTGAATTATCTTACTTAAATGGCTCAGAGGACTCTGGTCGTGG; CAGAGGTATTAGAGGCAGAGGGATCAGAATAGCTCCCACAGCTCCTTCAAGGGGCCGTGGGGGTGCCATT; CCTCCTCCCCCACCACCTGGACGAGGTGTTCTCACCCCTCGGGGAAGCACTGTAACCCGTGGAGCGCTTC; CAGTGCCACCTGTAGCAAGAGGTGTCCCTACCCCTCGAGCCCGGGGGGCACCAACAGTGCCAGGATACAG; GGCACCTCCTCCTCCAGCCCATGAAGCTTATGAAGAATATGGTTATGATGATGGCTACGGGGGTGAATAT; GATGACCAGACCTATGAGACTTATGATAACAGCTACGCGACCCAAACACAAAGTGTGCCTGAATACTATG; ACTACGGTCATGGAGTAAGTGAGGATGCCTATGACAGCTACGCACCAGAAGAATGGGCCACAACCAGCTC; TAGCTTGAAGGCACCACCGCAAAGGTCAGCCAGAGGGGGATACAGGGAACACCCCTATGGTAGATAT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205428 protein sequence; Red=Cloning site Green=Tags(s); ; MEEEKYLPELMAEKDSLDPSFVHASRLLAEEIEKFQGSDGKKEDEEKKYLDVISNKNIKLSERVLIPVKQ; YPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKAKEEELRKSGEAKYAHLSDELHVLIEVFAP; PGEAYSRMSHALEEIKKFLVPDYNDEIRQEQLRELSYLNGSEDSGRGRGIRGRGIRIAPTAPSRGRGGAI; PPPPPPGRGVLTPRGSTVTRGALPVPPVARGVPTPRARGAPTVPGYRAPPPPAHEAYEEYGYDDGYGGEY; DDQTYETYDNSYATQTQSVPEYYDYGHGVSEDAYDSYAPEEWATTSSSLKAPPQRSARGGYREHPYGRY; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152688
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152688.1, NP_689901.1
• RefSeq Size: 2336 bp
• RefSeq ORF: 1050 bp
• Locus ID: 202559
• UniProt ID: Q5VWX1
• Cytogenetics: 6q11.1
• Domains: KH
• MW: 38.9 kDa
• Summary: RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds both poly(A) and poly(U) homopolymers. Phosphorylation by PTK6 inhibits its RNA-binding ability (By similarity). Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. Can regulate alternative splicing of NRXN1 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. Regulates cell-type specific alternative splicing of NRXN1 at AS4 and acts synergystically with SAM68 in exon skipping. In contrast acts antagonistically with SAM68 in NRXN3 exon skipping at AS4. Its phosphorylation by FYN inhibits its ability to regulate splice site selection. May function as an adapter protein for Src kinases during mitosis.[UniProtKB/Swiss-Prot Function]