TEX47 (NM_152706) Human Tagged ORF Clone

SKU
RC205422
C7orf62 (Myc-DDK-tagged)-Human chromosome 7 open reading frame 62 (C7orf62)
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TEX47
Synonyms C7orf62
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205422 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTTTTCGGTCCATAACCAGAAGGGCAGCAAAAGGCCTTTGCCACTGGAACCTCTTCTTTTTCTCC
AAGTCCCACGTAGCAATTACCTGCACTTTCAAGAAGAGAAACAACGACTACACCTAAAGAAATTCCTTCT
TGATAGGATGTTTCTAGTGGCCAAGATACAAGCAAATGTAGAAAGAAAAGATGTTGCTGACTACTATGAA
CAAATGTTTCAGTCAGTTTTGAAACATCACCTAGGAGAAGCAGTGACAGGATTGCTGCTCATCTATCCCA
CTTCCATTCTGCATATCCTCGAGTCCTCCAGCGACACTCTCTACAAAGTTCTTTTAGATTATATTGGCCA
TGTCAAAGATGAAACAGTATTTTTTATTCAACAAATGAAAATTATAGTCATTTCTCATAACATTCCAATG
AGGCTTTTTATGCAATGGCATGTTTCAGTGATAAAAGTTCCAGTTATGTATCTCGACGATGTGACACAGT
CACAGTCCCTAAAGGAGGTCATCACAGATTTTCTCACACAAACTCATAAACTGTCACTCTACCTTTGCCA
GACTATGAAAGTAGGCACTAAAGGACCAGGCGATAACTTACACCAAGTTGCACCTGACCTACTCCTCCCA
GAACAAATCATAAAGTACTTGTGCAAATCCGAAGAATTCATGGACCCGGCAACATTTATAAACATGTATA
ATAGACCCATACACATCACTCTGGATTCTGAGGTGGTATGGCCTGCTCCTTCACGTTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205422 protein sequence
Red=Cloning site Green=Tags(s)

MSFSVHNQKGSKRPLPLEPLLFLQVPRSNYLHFQEEKQRLHLKKFLLDRMFLVAKIQANVERKDVADYYE
QMFQSVLKHHLGEAVTGLLLIYPTSILHILESSSDTLYKVLLDYIGHVKDETVFFIQQMKIIVISHNIPM
RLFMQWHVSVIKVPVMYLDDVTQSQSLKEVITDFLTQTHKLSLYLCQTMKVGTKGPGDNLHQVAPDLLLP
EQIIKYLCKSEEFMDPATFINMYNRPIHITLDSEVVWPAPSRF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_152706
ORF Size 759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152706.4
RefSeq Size 1038 bp
RefSeq ORF 762 bp
Locus ID 219557
UniProt ID Q8TBZ9
Cytogenetics 7q21.13
MW 29.5 kDa
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Citations

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