FACL4 (ACSL4) (NM_004458) Human Tagged ORF Clone

SKU RC205356

ACSL4 (Myc-DDK-tagged)-Human acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FACL4
• Synonyms: ACS4; FACL4; LACS4; MRX63; MRX68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205356 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAAAGAGAATAAAAGCTAAGCCCACTTCAGACAAACCTGGAAGTCCATATCGCTCTGTCACACACT; TCGACTCACTAGCTGTAATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTATC; CAAGTTTGGGAAGAAGGACAGCCTTGGGACCAGGGAAATCCTAAGTGAAGAAAATGAAATGCAGCCAAAT; GGAAAAGTTTTTAAGAAGTTAATTCTTGGGAATTATAAATGGATGAACTATCTTGAAGTGAATCGCAGAG; TGAATAACTTTGGTAGTGGACTCACTGCACTGGGACTAAAACCAAAGAACACCATTGCCATCTTCTGTGA; GACCAGGGCCGAATGGATGATTGCAGCACAGACCTGCTTTAAGTACAACTTTCCTCTTGTGACTTTATAT; GCCACACTTGGCAAAGAAGCAGTAGTTCATGGGCTAAATGAATCTGAGGCTTCCTATCTGATTACCAGTG; TTGAACTTCTGGAAAGTAAACTTAAGACTGCATTGTTAGATATCAGTTGTGTTAAACATATCATTTATGT; GGACAATAAGGCTATCAATAAAGCAGAGTACCCTGAAGGATTTGAGATTCACAGCATGCAATCAGTAGAA; GAGTTGGGATCTAACCCAGAAAACTTGGGCATTCCTCCAAGTAGACCAACGCCTTCAGACATGGCCATTG; TTATGTATACTAGTGGTTCTACTGGCCGACCTAAGGGAGTGATGATGCATCATAGCAATTTGATAGCTGG; AATGACAGGCCAGTGTGAAAGAATACCTGGACTGGGACCGAAGGACACATATATTGGCTACTTGCCTTTG; GCTCATGTGCTAGAACTGACAGCAGAGATATCTTGCTTTACCTATGGCTGCAGGATTGGATATTCTTCTC; CGCTTACACTCTCTGACCAGTCCAGCAAAATTAAAAAAGGAAGCAAAGGAGACTGTACTGTACTGAAGCC; CACACTTATGGCTGCTGTTCCGGAAATCATGGATAGAATTTATAAGAATGTTATGAGCAAAGTCCAAGAG; ATGAATTATATTCAGAAAACTCTGTTCAAGATAGGGTATGATTACAAATTGGAACAGATCAAAAAGGGAT; ATGATGCACCTCTTTGCAATCTGTTACTGTTTAAAAAGGTCAAGGCCCTGCTGGGAGGGAATGTCCGCAT; GATGCTGTCTGGAGGGGCCCCGCTATCTCCTCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCA; ATTGGCCAGGGTTATGGACTGACAGAATCATGTGGTGCTGGGACAGTTACTGAAGTAACTGACTATACTA; CTGGCAGAGTTGGAGCACCTCTTATTTGCTGTGAAATTAAGCTAAAAGACTGGCAAGAAGGCGGTTATAC; AATTAATGACAAGCCAAACCCCAGAGGTGAAATCGTAATTGGTGGACAGAACATCTCCATGGGATATTTT; AAAAATGAAGAGAAAACAGCAGAAGATTATTCTGTGGATGAAAATGGACAAAGGTGGTTTTGCACTGGTG; ATATTGGAGAATTCCATCCCGATGGATGTTTACAGATTATAGATCGTAAGAAAGATCTAGTGAAGTTACA; AGCAGGAGAGTATGTATCTCTTGGGAAAGTGGAAGCTGCACTGAAGAATTGTCCACTTATTGACAACATC; TGTGCTTTTGCCAAAAGTGATCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAGGTTGACAC; TTTTGGCACAACAGAAAGGGGTAGAAGGAACTTGGGTTGATATCTGCAATAATCCTGCTATGGAAGCTGA; AATACTGAAAGAAATTCGAGAAGCTGCAAATGCCATGAAATTGGAGCGATTTGAAATTCCAATCAAGGTT; CGATTAAGCCCAGAGCCATGGACCCCTGAAACTGGTTTGGTAACTGATGCTTTCAAACTGAAAAGGAAGG; AGCTGAGGAACCATTACCTCAAAGACATTGAACGAATGTATGGGGGCAAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205356 protein sequence; Red=Cloning site Green=Tags(s); ; MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPN; GKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLY; ATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVE; ELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPL; AHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQE; MNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCP; IGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYF; KNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNI; CAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKV; RLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMYGGK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004458
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004458.3
• RefSeq Size: 5039 bp
• RefSeq ORF: 2013 bp
• Locus ID: 2182
• UniProt ID: O60488
• Cytogenetics: Xq23
• Domains: AMP-binding
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• MW: 74.4 kDa
• Summary: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]