AK3 (NM_016282) Human Tagged ORF Clone

SKU
RC204408
AK3 (Myc-DDK-tagged)-Human adenylate kinase 3 (AK3), nuclear gene encoding mitochondrial protein, transcript variant 1
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Specifications
Product Data
Target Symbol AK3
Synonyms AK3L1; AK6; AKL3L; AKL3L1; FIX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204408 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCGTCCGCGCGGCTGCTGCGAGCGGTGATCATGGGGGCCCCGGGCTCGGGCAAGGGCACCGTGT
CGTCGCGCATCACTACACACTTCGAGCTGAAGCACCTCTCCAGCGGGGACCTGCTCCGGGACAACATGCT
GCGGGGCACAGAAATTGGCGTGTTAGCCAAGGCTTTCATTGACCAAGGGAAACTCATCCCAGATGATGTC
ATGACTCGGCTGGCCCTTCATGAGCTGAAAAATCTCACCCAGTATAGCTGGCTGTTGGATGGTTTTCCAA
GGACACTTCCACAGGCAGAAGCCCTAGATAGAGCTTATCAGATCGACACAGTGATTAACCTGAATGTGCC
CTTTGAGGTCATTAAACAACGCCTTACTGCTCGCTGGATTCATCCCGCCAGTGGCCGAGTCTATAACATT
GAATTCAACCCTCCCAAAACTGTGGGCATTGATGACCTGACTGGGGAGCCTCTCATTCAGCGTGAGGATG
ATAAACCAGAGACGGTTATCAAGAGACTAAAGGCTTATGAAGACCAAACAAAGCCAGTCCTGGAATATTA
CCAGAAAAAAGGGGTGCTGGAAACATTCTCCGGAACAGAAACCAACAAGATTTGGCCCTATGTATATGCT
TTCCTACAAACTAAAGTTCCACAAAGAAGCCAGAAAGCTTCAGTTACTCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204408 protein sequence
Red=Cloning site Green=Tags(s)

MGASARLLRAVIMGAPGSGKGTVSSRITTHFELKHLSSGDLLRDNMLRGTEIGVLAKAFIDQGKLIPDDV
MTRLALHELKNLTQYSWLLDGFPRTLPQAEALDRAYQIDTVINLNVPFEVIKQRLTARWIHPASGRVYNI
EFNPPKTVGIDDLTGEPLIQREDDKPETVIKRLKAYEDQTKPVLEYYQKKGVLETFSGTETNKIWPYVYA
FLQTKVPQRSQKASVTP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_016282
ORF Size 681 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016282.4
RefSeq Size 4333 bp
RefSeq ORF 684 bp
Locus ID 50808
UniProt ID Q9UIJ7
Cytogenetics 9p24.1
Domains ADK, ADK_lid
Protein Families Druggable Genome
Protein Pathways Pyrimidine metabolism
MW 25.6 kDa
Summary The protein encoded by this gene is a GTP:ATP phosphotransferase that is found in the mitochondrial matrix. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
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SKU Description Size Price
RC204408L1 Lenti ORF clone of Human adenylate kinase 3 (AK3), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
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RC204408L3 Lenti ORF clone of Human adenylate kinase 3 (AK3), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
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RC204408L4 Lenti ORF clone of Human adenylate kinase 3 (AK3), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
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Citations

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