KLHL22 (NM_032775) Human Tagged ORF Clone

SKU RC204296

KLHL22 (Myc-DDK-tagged)-Human kelch-like 22 (Drosophila) (KLHL22), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: KLHL22
• Synonyms: KELCHL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204296 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGAGGAGCAGGAGTTCACCCAGCTCTGCAAGTTGCCTGCACAGCCCTCACACCCACACTGCGTGA; ACAACACCTACCGCAGCGCACAGCACTCCCAGGCTCTGCTCCGAGGGCTGCTGGCTCTCCGGGACAGCGG; AATCCTCTTCGATGTTGTGCTGGTGGTGGAGGGCAGACACATCGAGGCCCATCGCATCCTGCTGGCTGCG; TCCTGCGATTACTTCAGAGGAATGTTTGCTGGGGGATTGAAGGAGATGGAACAGGAAGAGGTCCTGATCC; ACGGTGTGTCCTACAATGCTATGTGCCAAATCCTACATTTCATATACACCTCCGAGCTGGAGCTCAGCCT; GAGCAATGTACAAGAGACACTGGTGGCTGCCTGCCAGCTTCAGATCCCAGAAATTATCCATTTCTGCTGT; GATTTCCTCATGTCCTGGGTGGACGAAGAGAACATTCTCGATGTCTACCGGCTGGCAGAGCTGTTTGACT; TGAGCCGCCTGACTGAGCAACTGGACACCTATATCCTCAAAAACTTTGTGGCCTTCTCTCGGACTGACAA; GTACCGCCAGCTTCCATTGGAGAAGGTCTACTCCCTCCTCAGCAGCAATCGCCTGGAGGTCTCCTGCGAG; ACCGAGGTATATGAGGGGGCCCTTCTCTACCATTATAGCCTGGAGCAGGTGCAGGCTGACCAGATCTCGC; TGCACGAGCCCCCAAAGCTCCTTGAGACAGTGCGGTTTCCGCTGATGGAAGCTGAGGTCCTGCAGCGGCT; GCATGACAAGCTGGACCCCAGCCCTTTGAGGGACACAGTGGCCAGCGCCCTCATGTACCACCGGAACGAG; AGCCTACAGCCCAGCCTGCAGAGCCCGCAAACGGAGCTGCGGTCGGACTTCCAGTGCGTTGTGGGCTTCG; GGGGCATTCACTCCACGCCGTCCACTGTCCTCAGCGACCAGGCCAAGTATCTAAACCCCTTACTGGGAGA; GTGGAAGCACTTCACTGCCTCCCTGGCCCCCCGCATGTCCAACCAGGGCATCGCGGTGCTCAACAACTTC; GTATACTTGATTGGAGGGGACAACAATGTCCAAGGATTTCGAGCAGAGTCCCGATGCTGGAGGTATGACC; CACGGCACAACCGCTGGTTCCAGATCCAGTCCCTGCAGCAGGAGCACGCCGACCTGTCCGTGTGTGTTGT; AGGCAGGTACATCTACGCTGTGGCGGGCCGTGACTACCACAATGACCTGAATGCTGTGGAGCGCTACGAC; CCTGCCACCAACTCCTGGGCATACGTGGCCCCACTCAAGAGGGAGGTGTATGCCCACGCAGGCGCGACGC; TGGAGGGGAAGATGTATATCACCTGCGGCCGCAGAGGGGAGGATTACCTGAAAGAGACACACTGCTACGA; TCCAGGCAGCAACACTTGGCACACACTGGCTGATGGGCCTGTGCGGCGCGCCTGGCACGGCATGGCAACC; CTCCTCAACAAGCTGTATGTGATCGGGGGCAGCAACAACGATGCCGGATACAGGAGGGACGTGCACCAGG; TGGCCTGCTACAGCTGCACGTCTGGACAGTGGTCATCTGTCTGCCCACTCCCTGCTGGGCACGGTGAGCC; TGGCATTGCTGTGCTGGACAACAGGATCTATGTGTTAGGTGGCCGCTCACACAACCGCGGCAGCCGCACA; GGCTACGTGCACATTTACGATGTGGAGAAGGACTGCTGGGAGGAAGGGCCCCAGCTGGACAACTCCATCT; CAGGCCTGGCGGCCTGTGTGCTCACCCTGCCCCGCTCCCTGCTCCTTGAGCCGCCCCGCGGGACCCCTGA; CCGCAGCCAGGCCGACCCGGACTTTGCCTCTGAGGTGATGAGTGTGTCTGACTGGGAGGAGTTTGACAAC; TCCAGTGAGGAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204296 protein sequence; Red=Cloning site Green=Tags(s); ; MAEEQEFTQLCKLPAQPSHPHCVNNTYRSAQHSQALLRGLLALRDSGILFDVVLVVEGRHIEAHRILLAA; SCDYFRGMFAGGLKEMEQEEVLIHGVSYNAMCQILHFIYTSELELSLSNVQETLVAACQLQIPEIIHFCC; DFLMSWVDEENILDVYRLAELFDLSRLTEQLDTYILKNFVAFSRTDKYRQLPLEKVYSLLSSNRLEVSCE; TEVYEGALLYHYSLEQVQADQISLHEPPKLLETVRFPLMEAEVLQRLHDKLDPSPLRDTVASALMYHRNE; SLQPSLQSPQTELRSDFQCVVGFGGIHSTPSTVLSDQAKYLNPLLGEWKHFTASLAPRMSNQGIAVLNNF; VYLIGGDNNVQGFRAESRCWRYDPRHNRWFQIQSLQQEHADLSVCVVGRYIYAVAGRDYHNDLNAVERYD; PATNSWAYVAPLKREVYAHAGATLEGKMYITCGRRGEDYLKETHCYDPGSNTWHTLADGPVRRAWHGMAT; LLNKLYVIGGSNNDAGYRRDVHQVACYSCTSGQWSSVCPLPAGHGEPGIAVLDNRIYVLGGRSHNRGSRT; GYVHIYDVEKDCWEEGPQLDNSISGLAACVLTLPRSLLLEPPRGTPDRSQADPDFASEVMSVSDWEEFDN; SSED; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032775
• ORF Size: 1902 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032775.4
• RefSeq Size: 2637 bp
• RefSeq ORF: 1905 bp
• Locus ID: 84861
• UniProt ID: Q53GT1
• Cytogenetics: 22q11.21
• Domains: BTB, Kelch
• MW: 71.7 kDa
• Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (PubMed:19995937, PubMed:23455478). The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy (PubMed:29769719).[UniProtKB/Swiss-Prot Function]