GADD45A (NM_001924) Human Tagged ORF Clone
GADD45A (Myc-DDK-tagged)-Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1
|Product Name||GADD45A (NM_001924) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC204005 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC204005 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||495 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001924.1, NM_001924.2, NM_001924.3, NP_001915.1|
|RefSeq Size||1398 bp|
|RefSeq ORF||498 bp|
|Protein Families||Druggable Genome, Stem cell - Pluripotency|
|Protein Pathways||Cell cycle, MAPK signaling pathway, p53 signaling pathway|
|Gene Summary||This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]|
|The use of this cDNA Clones has been cited in the following citations:|
GADD45α-targeted suicide gene therapy driven by synthetic CArG promoter E9NS sensitizes NSCLC cells to cisplatin, resveratrol, and radiation regardless of p53 status
,Shi, Q;Sutariya, V;Varghese Gupta, S;Bhatia, D;,
Onco Targets Ther 2019
,PubMed ID 31114253
An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
,Li, J;Dong, J;Li, S;Xia, W;Su, X;Qin, X;Chen, Y;Ding, H;Li, H;Huang, A;Bai, C;Hu, T;Wang, C;Chu, B;Shao, N;,
Sci Rep 2017
,PubMed ID 28769054
Gadd45a sensitizes medulloblastoma cells to irradiation and suppresses MMP-9–mediated EMT
,Swapna Asuthkar, Arun Kumar Nalla, Christopher S. Gondi, Dzung H. Dinh, Meena Gujrati, Sanjeeva Mohanam, and Jasti S. Rao,
Neuro Oncology, Oct 2011; 13: 1059 - 1073.
|cDNA Clone Resources|
|SC118947||GADD45A (untagged)-Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1||
|RG204005||GADD45A (GFP-tagged) - Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1||
|RC204005L1||Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, Myc-DDK-tagged||
|RC204005L2||Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, mGFP tagged||
|RC204005L3||Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, Myc-DDK-tagged||
|RC204005L4||Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, mGFP tagged||