PLEKHO1 (NM_016274) Human Tagged ORF Clone

SKU RC203969

PLEKHO1 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family O member 1 (PLEKHO1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PLEKHO1
• Synonyms: CKIP-1; CKIP1; JBP; OC120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203969 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATGAAGAAGAACAATTCCGCCAAGCGGGGACCTCAGGATGGAAACCAGCAGCCTGCACCGCCCGAGA; AGGTCGGCTGGGTCCGGAAATTCTGCGGGAAAGGGATTTTCAGGGAGATTTGGAAAAACCGCTATGTGGT; GCTGAAAGGGGACCAGCTCTACATCTCTGAGAAGGAGGTAAAAGATGAGAAAAATATTCAAGAGGTATTT; GACCTGAGTGACTATGAGAAGTGTGAAGAGCTCCGGAAGTCCAAGAGCAGGAGCAAGAAAAATCATAGCA; AGTTTACTCTTGCCCACTCCAAACAGCCCGGTAACACGGCACCCAACCTGATCTTCCTGGCAGTGAGTCC; AGAAGAGAAGGAATCGTGGATCAATGCCCTCAACTCTGCCATCACCCGAGCCAAGAACCGTATCTTGGAT; GAGGTCACCGTTGAGGAGGACAGCTATCTTGCCCATCCCACTCGAGACAGGGCAAAAATCCAGCACTCCC; GCCGCCCCCCAACAAGGGGACACCTAATGGCTGTGGCTTCCACCTCTACCTCGGATGGGATGCTGACCTT; GGACTTGATCCAAGAGGAAGACCCTTCCCCTGAGGAACCAACCTCTTGTGCTGAGAGCTTTCGGGTTGAC; CTGGACAAGTCTGTGGCCCAGCTGGCAGGGAGCCGGCGGAGAGCGGACTCAGACCGCATCCAGCCCTCCG; CAGACCGGGCAAGCAGTCTCTCCCGACCTTGGGAAAAAACAGACAAAGGGGCCACCTACACCCCCCAGGC; ACCCAAGAAGTTGACGCCCACAGAGAAAGGCCGCTGCGCCTCCCTGGAGGAGATCCTATCTCAGCGGGAT; GCTGCCTCTGCCCGCACCCTCCAGCTGCGGGCTGAGGAACCCCCAACCCCTGCCCTCCCCAACCCGGGGC; AGCTGTCCCGGATCCAGGACCTGGTAGCAAGGAAACTGGAGGAGACTCAGGAGCTTCTGGCAGAGGTTCA; GGGACTGGGAGATGGGAAGCGAAAGGCCAAGGACCCCCCTCGGTCTCCGCCGGATTCTGAGTCAGAGCAG; CTGCTGCTGGAGACGGAACGGCTGCTGGGAGAGGCATCATCGAATTGGAGCCAGGCAAAGAGGGTGCTGC; AGGAGGTCAGGGAGCTGAGAGACCTGTACAGACAGATGGACCTGCAGACCCCGGACTCCCACCTCAGACA; GACCACCCCGCACAGTCAGTACCGGAAGAGCCTGATG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203969 protein sequence; Red=Cloning site Green=Tags(s); ; MMKKNNSAKRGPQDGNQQPAPPEKVGWVRKFCGKGIFREIWKNRYVVLKGDQLYISEKEVKDEKNIQEVF; DLSDYEKCEELRKSKSRSKKNHSKFTLAHSKQPGNTAPNLIFLAVSPEEKESWINALNSAITRAKNRILD; EVTVEEDSYLAHPTRDRAKIQHSRRPPTRGHLMAVASTSTSDGMLTLDLIQEEDPSPEEPTSCAESFRVD; LDKSVAQLAGSRRRADSDRIQPSADRASSLSRPWEKTDKGATYTPQAPKKLTPTEKGRCASLEEILSQRD; AASARTLQLRAEEPPTPALPNPGQLSRIQDLVARKLEETQELLAEVQGLGDGKRKAKDPPRSPPDSESEQ; LLLETERLLGEASSNWSQAKRVLQEVRELRDLYRQMDLQTPDSHLRQTTPHSQYRKSLM; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016274
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016274.6
• RefSeq Size: 1627 bp
• RefSeq ORF: 1230 bp
• Locus ID: 51177
• UniProt ID: Q53GL0
• Cytogenetics: 1q21.2
• Domains: PH
• MW: 46.2 kDa
• Summary: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.[UniProtKB/Swiss-Prot Function]