BORCS5 (NM_058169) Human Tagged ORF Clone
CAT#: RC203844
LOH12CR1 (Myc-DDK-tagged)-Human loss of heterozygosity, 12, chromosomal region 1 (LOH12CR1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_058169" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | BORCS5 |
Synonyms | LOH1CR12; LOH12CR1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203844 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGTGAGCAGAGCTCCGAGGCCGAGAGCCGACCCAACGATCTGAACTCCTCAGTGACTCCTTCAC CAGCCAAGCATAGAGCCAAGATGGATGATATTGTGGTTGTAGCTCAGGGCTCCCAGGCCTCACGGAACGT CAGCAACGATCCCGATGTCATCAAGTTGCAAGAGATTCCAACCTTCCAGCCCCTTTTGAAAGGGCTATTG AGTGGCCAGACTTCCCCAACAAATGCCAAATTGGAGAAACTGGACTCTCAGCAGGTGTTGCAGCTCTGCC TCCGATATCAAGATCACCTGCATCAGTGTGCAGAGGCCGTTGCTTTTGACCAGAATGCTTTGGTTAAACG AATCAAAGAGATGGATCTGTCTGTAGAAACTCTGTTCAGCTTCATGCAGGAGCGCCAGAAAAGATACGCC AAGTATGCCGAGCAGATCCAGAAAGTGAACGAGATGTCCGCCATCCTCCGCCGCATACAGATGGGCATCG ACCAGACTGTGCCCCTGCTGGACAGGCTCAACAGCATGCTGCCCGAGGGCGAGCGGCTGGAGCCCTTCAG CATGAAGCCCGACCGCGAGCTCAGGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203844 protein sequence
Red=Cloning site Green=Tags(s) MGSEQSSEAESRPNDLNSSVTPSPAKHRAKMDDIVVVAQGSQASRNVSNDPDVIKLQEIPTFQPLLKGLL SGQTSPTNAKLEKLDSQQVLQLCLRYQDHLHQCAEAVAFDQNALVKRIKEMDLSVETLFSFMQERQKRYA KYAEQIQKVNEMSAILRRIQMGIDQTVPLLDRLNSMLPEGERLEPFSMKPDRELRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_058169 |
ORF Size | 588 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_058169.6 |
RefSeq Size | 2092 bp |
RefSeq ORF | 591 bp |
Locus ID | 118426 |
UniProt ID | Q969J3 |
Cytogenetics | 12p13.2 |
MW | 22.2 kDa |
Gene Summary | As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor. Thereby, it may indirectly play a role in cell spreading and motility.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203844L3 | Lenti ORF clone of Human loss of heterozygosity, 12, chromosomal region 1 (LOH12CR1), Myc-DDK-tagged |
USD 600.00 |
|
RC203844L4 | Lenti ORF clone of Human loss of heterozygosity, 12, chromosomal region 1 (LOH12CR1), mGFP tagged |
USD 600.00 |
|
RG203844 | LOH12CR1 (tGFP-tagged) - Human loss of heterozygosity, 12, chromosomal region 1 (LOH12CR1) |
USD 500.00 |
|
SC120239 | LOH12CR1 (untagged)-Human loss of heterozygosity, 12, chromosomal region 1 (LOH12CR1) |
USD 300.00 |
|
SC324648 | LOH12CR1 (untagged)-Human loss of heterozygosity, 12, chromosomal region 1 (LOH12CR1) |
USD 300.00 |
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