BORCS5 (NM_058169) Human Untagged Clone

CAT#: SC120239

LOH12CR1 (untagged)-Human loss of heterozygosity, 12, chromosomal region 1 (LOH12CR1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BORCS5
• Synonyms: LOH1CR12; LOH12CR1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_058169, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAGTGAGCAGAGCTCCGAGGCCGAGAGCCGACCCAACGATCTGAACTCCTCAGTGACTCCTTCAC
  CAGCCAAGCATAGAGCCAAGATGGATGATATTGTGGTTGTAGCTCAGGGCTCCCAGGCCTCACGGAACGT
  CAGCAACGATCCCGATGTCATCAAGTTGCAAGAGATTCCAACCTTCCAGCCCCTTTTGAAAGGGCTATTG
  AGTGGCCAGACTTCCCCAACAAATGCCAAATTGGAGAAACTGGACTCTCAGCAGGTGTTGCAGCTCTGCC
  TCCGATATCAAGATCACCTGCATCAGTGTGCAGAGGCCGTTGCTTTTGACCAGAATGCTTTGGTTAAACG
  AATCAAAGAGATGGATCTGTCTGTAGAAACTCTGTTCAGCTTCATGCAGGAGCGCCAGAAAAGATACGCC
  AAGTATGCCGAGCAGATCCAGAAAGTGAACGAGATGTCCGCCATCCTCCGCCGCATACAGATGGGCATCG
  ACCAGACTGTGCCCCTGCTGGACAGGCTCAACAGCATGCTGCCCGAGGGCGAGCGGCTGGAGCCCTTCAG
  CATGAAGCCCGACCGCGAGCTCAGGCTGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_058169 unedited
  GTCAACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGCCCCGTCCCGG
  TCCCTGGCCCCTGCCCTGTCGCCCGCCGCCGGAGCGGTGACCGCCCGGCCCGCCGTTCTT
  CTGCTGCCACCGCTGTCGGCACCATGGGCAGTGAGCAGAGCTCCGAGGCCGAGAGCCGAC
  CCAACGATCTGAACTCCTCAGTGACTCCTTCACCAGCCAAGCATAGAGCCAAGATGGATG
  ATATTGTGGTTGTAGCTCAGGGCTCCCAGGCCTCACGGAACGTCAGCAACGATCCCGATG
  TCATCAAGTTGCAAGAGATTCCAACCTTCCAGCCCCTTTTGAAAGGGCTATTGAGTGGCC
  AGACTTCCCCAACAAATGCCAAATTGGAGAAACTGGACTCTCAGCAGGTGTTGCAGCTCT
  GCCTCCGATATCAAGATCACCTGCATCAGTGTGCAGAGGCCGTTGCTTTTGACCAGAATG
  CTTTGGTTAAACGAATCAAAGAGGTAATGTGCTGCGGGAAAATAACATTGCTGACTGGTT
  GTTGTGATTCTCTGCAACTCTGGATGCCATCTTAAGACTTGACATTTGTCACTATTGCTT
  TGAGGAGATTGCTTTTTCCCAGCAGTAAAATGTGATGATGAAAAAAGTGGTCCACAGAGC
  TGGGCTAATTTAGAGTTTGAATTCTGATCACCACTGATTTGCTTGGCCAAGTTGCTTAAC
  TTCTCTGTCTCAGTTTCCTCATGTGAAAAATGGNGCTAATAGTAGTAACTTACCCCAAGG
  GNTGAGTGACAGATTTGCTAGTAAAGTGTAGCTATTATTAGTGCATGGTAAGTCCTGCCA
  TACTAATTTGCTCTAGCCCCATTCTGTCTTTGAATTGACTTAAATGTTCCTGTGTATAAT
  TTATATTTGGTTCCCAGTAGATTAAAATTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_058169
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_058169.2, NP_477517.1
• RefSeq Size: 2049 bp
• RefSeq ORF: 591 bp
• Locus ID: 118426
• UniProt ID: Q969J3
• Cytogenetics: 12p13.2
• Gene Summary: As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor. Thereby, it may indirectly play a role in cell spreading and motility.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.