MRAP2 (NM_138409) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MRAP2 |
| Synonyms | bA51G5.2; C6orf117 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_138409 |
| ORF Size | 615 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_138409.4 |
| RefSeq Size | 2229 bp |
| RefSeq ORF | 618 bp |
| Locus ID | 112609 |
| UniProt ID | Q96G30 |
| Cytogenetics | 6q14.2 |
| Protein Families | Transmembrane |
| MW | 23.5 kDa |
| Summary | This gene encodes a protein that modulates melanocortin receptor signaling. The encoded protein has been shown to interact with all known melanocortin receptors and may regulate both receptor trafficking and activation in response to ligands. Mice lacking a functional copy of this gene exhibit severe obesity and a mutation in this gene may be associated with severe obesity in human patients. provided by RefSeq, Oct 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_138409" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203668L1 | Lenti ORF clone of Human melanocortin 2 receptor accessory protein 2 (MRAP2), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC203668L2 | Lenti ORF clone of Human melanocortin 2 receptor accessory protein 2 (MRAP2), mGFP tagged | 10 ug |
$600.00
|
|
| RC203668L3 | Lenti ORF clone of Human melanocortin 2 receptor accessory protein 2 (MRAP2), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC203668L4 | Lenti ORF clone of Human melanocortin 2 receptor accessory protein 2 (MRAP2), mGFP tagged | 10 ug |
$600.00
|
|
| RG203668 | MRAP2 (tGFP-tagged) - Human melanocortin 2 receptor accessory protein 2 (MRAP2) | 10 ug |
$489.00
$500.00
|
|
| SC100506 | MRAP2 (untagged)-Human melanocortin 2 receptor accessory protein 2 (MRAP2) | 10 ug |
$300.00
|
|
Citations
MRAP2 Inhibits β-Arrestin-2 Recruitment to the Prokineticin Receptor 2
Lattanzi, Casella, Fullone et al
Curr Issues Mol Biol (2024) 46 (2), 1607-1620 DOI: 10.3390/cimb46020104
Lattanzi, Casella, Fullone et al
Curr Issues Mol Biol (2024) 46 (2), 1607-1620 DOI: 10.3390/cimb46020104
Cited by 11
AI Summary: OriGene contributed to this study by providing the MRAP2 expression plasmid (RC203668), which was used to investigate the role of MRAP2 in modulating β-arrestin-2 recruitment to the prokineticin receptor 2.
Snippet: Luc was described in [38]. For crosslinking experiments to express the PKR2-ΔC52 mutant, we used PKR2-ΔC52 pcDNA described in [38] and to express MRAP2, we used the RC203668 plasmid purchased from Origene (Thermo Fisher Scientific, Grand Island, NY, USA). For the expression of hMRAP2-Glutathione S-Transferase (GST), hMRAP2-CTpBS was digested with BamHI and EcoRI, and the obtained fragment was
A human obesity-associated MC4R mutation with defective Gq/11α signaling leads to hyperphagia in mice
Metzger, Zhang, Carlson et al
J Clin Invest (2024)
Metzger, Zhang, Carlson et al
J Clin Invest (2024)
AI Summary: OriGene’s mouse anti-DDK (FLAG) monoclonal antibody (TA50011-100) was used in this study to evaluate the expression of DDK-Myc-tagged Human MRAP2 by immunoblotting, thereby verifying successful exogenous protein expression in the experimental cell system.
Snippet: The exogenous expression of DDK-Myc-tagged Human MRAP2 in the stable cell pool was 551 evaluated by immunoblotting membrane fractions with mouse anti-DDK (FLAG) monoclonal 552 antibody (1:250, TA50011-100, OriGene) and anti-a-tubulin antibody (CP06, Sigma-Aldrich) to 553 control for loading. MRAP2 expression was verified with quantitative RT-PCR (Forward primer: 554 5’-ATTTTCTCGCCAAGGCAACG-3’, reverse primer: 5’- TGCTTCTGATGGCTTCCTGG-3’)
Arginine 125 Is an Essential Residue for the Function of MRAP2
Fullone, Maftei, Vincenzi et al
Int J Mol Sci (2022) 23 (17) DOI: 10.3390/ijms23179853
Fullone, Maftei, Vincenzi et al
Int J Mol Sci (2022) 23 (17) DOI: 10.3390/ijms23179853
Cited by 12
AI Summary: OriGene’s MYC-DDK-tagged human MRAP2 expression plasmid (RC203668) was used to constitutively express MRAP2 in mammalian cell lines, enabling functional analysis of MRAP2 and its mutants in this study.
Snippet: BamHI up and MRAP2 EcoRI dw and cloned into p413 digested with BamHI and EcoRI. For constitutive expression of MYC-DDk-tagged human MRAP2 in mammalian cell lines, RC203668 plasmid was purchased from Origene (Fisher Scientific, Waltham, MA, USA). The p413 humanMRAP2 plasmid previously described in [5] was used as a template to construct the R125H and R125C MRAP2 mutants with the QuikChange II Sit
Identification of Regions Involved in the Physical Interaction between Melanocortin Receptor Accessory Protein 2 and Prokineticin Receptor 2
Fullone, Maftei, Vincenzi et al
Biomolecules (2022) 12 (3) DOI: 10.3390/biom12030474
Fullone, Maftei, Vincenzi et al
Biomolecules (2022) 12 (3) DOI: 10.3390/biom12030474
Cited by 17
AI Summary: OriGene’s MYC-DDK-tagged human MRAP2 expression plasmid (RC203668) was used to constitutively express MRAP2 in mammalian cell lines, enabling the study of its interaction with Prokineticin Receptor 2 in this paper.
Snippet: s and adipocyte tissue. 2. Materials and Methods 2.1. Expression Constructs For the constitutive expression of MYC-DDk-tagged human MRAP2 in mammalian cell lines, RC203668 plasmid was purchased from Origene (Fisher Scientific). For the expression of MRAP2 in Saccharomyces cerevisiae (S. cerevisiae), the p413 MRAP2 plasmid was constructed. The cDNA of MRAP2 was amplified by PCR using a as templat
Showing 1-4 of 4 papers.
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