SMCR7L (MIEF1) (NM_019008) Human Tagged ORF Clone

SKU RC203568

SMCR7L (Myc-DDK-tagged)-Human Smith-Magenis syndrome chromosome region, candidate 7-like (SMCR7L)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SMCR7L
• Synonyms: AltMIEF1; dJ1104E15.3; HSU79252; MID51; MIEF1-MP; SMCR7L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203568 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGGCGCTGGTGAGCGCAAAGGCAAGAAGGATGACAATGGCATTGGCACGGCCATTGACTTTGTGC; TCTCCAATGCCCGGCTGGTGCTGGGGGTGGGTGGAGCGGCCATGCTGGGCATCGCCACGCTGGCAGTTAA; GCGGATGTACGATCGGGCGATCAGTGCCCCTACCAGCCCCACCCGCCTGAGCCATTCGGGGAAAAGGAGC; TGGGAAGAACCCAACTGGATGGGCTCCCCACGACTGCTGAACAGGGACATGAAGACGGGCCTGAGCCGGT; CCTTGCAGACCCTTCCCACAGACTCCTCCACCTTCGACACAGATACATTCTGCCCGCCCCGGCCCAAGCC; AGTGGCCAGGAAGGGCCAGGTAGACTTGAAGAAGTCACGACTCCGCATGTCCCTGCAGGAGAAACTTCTT; ACTTACTACCGGAACCGGGCAGCCATCCCTGCTGGAGAGCAGGCTCGGGCCAAGCAAGCTGCTGTGGACA; TATGTGCCGAGCTCCGGAGCTTCCTGCGGGCCAAGTTGCCTGACATGCCGCTTCGGGACATGTACTTGAG; TGGCAGCCTCTACGATGACCTGCAGGTGGTGACAGCTGACCACATCCAACTCATTGTGCCCCTTGTGCTG; GAGCAGAACCTGTGGTCATGTATTCCTGGTGAAGACACCATCATGAATGTCCCTGGCTTCTTCCTGGTGC; GTCGTGAGAATCCAGAGTACTTTCCTCGTGGGAGCAGTTACTGGGACCGCTGTGTAGTAGGGGGCTACCT; CTCTCCAAAGACAGTCGCAGATACATTTGAGAAGGTAGTGGCTGGCTCCATCAATTGGCCAGCCATAGGG; TCCCTCTTGGACTATGTGATCCGCCCGGCCCCACCCCCAGAAGCCCTCACACTGGAGGTGCAGTATGAGC; GTGACAAACATCTCTTCATTGACTTCCTGCCATCAGTGACCCTCGGTGACACAGTCTTGGTGGCCAAACC; ACACCGGCTAGCCCAGTATGACAACCTGTGGCGGCTGAGCCTGCGTCCCGCGGAGACGGCACGCCTGCGG; GCTCTGGACCAGGCTGACTCGGGCTGCCGATCTCTGTGCCTCAAGATCCTCAAGGCCATATGCAAGTCCA; CCCCGGCTCTGGGCCACCTCACTGCCAGCCAGCTAACCAATGTCATCCTCCACTTGGCCCAGGAGGAGGC; TGACTGGTCTCCGGATATGCTGGCCGACCGTTTCCTGCAGGCCTTGAGGGGACTTATCAGCTACTTAGAG; GCTGGAGTCCTGCCCAGTGCCCTAAACCCCAAGGTGAACTTATTTGCAGAGCTCACCCCTGAAGAAATAG; ACGAATTAGGATACACTCTGTATTGCTCATTGTCTGAGCCAGAGGTGCTGCTGCAGACG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203568 protein sequence; Red=Cloning site Green=Tags(s); ; MAGAGERKGKKDDNGIGTAIDFVLSNARLVLGVGGAAMLGIATLAVKRMYDRAISAPTSPTRLSHSGKRS; WEEPNWMGSPRLLNRDMKTGLSRSLQTLPTDSSTFDTDTFCPPRPKPVARKGQVDLKKSRLRMSLQEKLL; TYYRNRAAIPAGEQARAKQAAVDICAELRSFLRAKLPDMPLRDMYLSGSLYDDLQVVTADHIQLIVPLVL; EQNLWSCIPGEDTIMNVPGFFLVRRENPEYFPRGSSYWDRCVVGGYLSPKTVADTFEKVVAGSINWPAIG; SLLDYVIRPAPPPEALTLEVQYERDKHLFIDFLPSVTLGDTVLVAKPHRLAQYDNLWRLSLRPAETARLR; ALDQADSGCRSLCLKILKAICKSTPALGHLTASQLTNVILHLAQEEADWSPDMLADRFLQALRGLISYLE; AGVLPSALNPKVNLFAELTPEEIDELGYTLYCSLSEPEVLLQT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019008
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019008.5
• RefSeq Size: 5730 bp
• RefSeq ORF: 1392 bp
• Locus ID: 54471
• UniProt ID: Q9NQG6
• Cytogenetics: 22q13.1
• Protein Families: Transmembrane
• MW: 51.3 kDa
• Summary: Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L.[UniProtKB/Swiss-Prot Function]