Ubiquinol Cytochrome C Reductase Core Protein I (UQCRC1) (NM_003365) Human Tagged ORF Clone

CAT#: RC203299

UQCRC1 (Myc-DDK-tagged)-Human ubiquinol-cytochrome c reductase core protein I (UQCRC1)

Product Images

Circular map for RC203299

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UQCRC1 (Cat# RC203299, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UQCRC1 (Cat# TA800089). Positive lysates LY401151 (100ug) and LC401151 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401151) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203299 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells transfected with either RC203299 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UQCRC1 antibody (TA800089), and then analyzed by flow cytometry.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ubiquinol Cytochrome C Reductase Core Protein I
• Synonyms: D3S3191; QCR1; UQCR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203299 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTCCGTGGTCTGTCGGGCCGCTACCGCCGGGGCACAAGTGCTATTGCGCGCCCGCCGCTCGC
  CGGCCCTGCTGCGGACGCCAGCCTTGCGGAGTACGGCAACCTTCGCTCAGGCGCTCCAGTTCGTGCCGGA
  GACGCAGGTTAGCCTGCTGGACAACGGCCTGCGTGTGGCCTCCGAGCAGTCCTCTCAGCCCACTTGCACG
  GTGGGAGTGTGGATTGATGTTGGCAGCCGTTTTGAGACTGAGAAGAATAATGGGGCAGGCTACTTTTTGG
  AGCATCTGGCTTTCAAGGGAACAAAGAATCGGCCTGGCAGTGCCCTGGAGAAGGAGGTGGAGAGCATGGG
  GGCCCATCTTAATGCCTACAGCACCCGGGAGCACACAGCTTACTACATCAAGGCGCTGTCCAAGGATCTG
  CCGAAAGCTGTGGAGCTCCTGGGTGACATTGTGCAGAACTGTAGTCTGGAAGACTCACAGATTGAGAAGG
  AACGTGATGTGATCCTGCGGGAGATGCAGGAGAATGATGCATCTATGCGAGATGTGGTCTTTAACTACCT
  GCATGCCACAGCATTCCAGGGCACACCTCTAGCCCAGGCTGTGGAGGGGCCCAGTGAGAATGTCAGGAAG
  CTGTCTCGTGCAGACTTGACCGAGTACCTCAGCACACATTACAAGGCCCCTCGAATGGTGCTGGCAGCAG
  CTGGAGGAGTGGAGCACCAGCAACTGTTAGACCTCGCCCAGAAGCACCTCGGTGGCATCCCATGGACATA
  TGCAGAGGACGCTGTGCCCACTCTTACTCCATGCCGCTTCACTGGCAGTGAGATCCGCCACCGTGATGAT
  GCTCTACCTTTTGCCCACGTGGCCATTGCAGTAGAGGGTCCTGGCTGGGCCAGCCCGGACAATGTGGCCT
  TGCAAGTGGCCAATGCCATCATCGGCCACTATGACTGCACTTATGGTGGTGGCGTGCACCTGTCCAGCCC
  ACTGGCTTCAGGTGCTGTGGCCAACAAGCTATGCCAGAGTTTCCAGACCTTCAGCATCTGCTATGCAGAG
  ACGGGCTTGCTGGGTGCACACTTTGTCTGTGACCGAATGAAAATCGATGACATGATGTTCGTCCTGCAAG
  GGCAGTGGATGCGCCTGTGTACCAGTGCCACGGAGAGTGAGGTGGCCCGGGGCAAAAACATCCTCAGAAA
  TGCCCTGGTATCTCATCTAGATGGCACTACTCCTGTGTGTGAGGACATCGGACGCAGCCTCCTGACCTAT
  GGCCGCCGCATCCCCCTGGCTGAATGGGAAAGCCGGATTGCGGAGGTGGATGCCAGTGTGGTACGTGAGA
  TCTGCTCCAAGTACATCTATGACCAGTGCCCAGCAGTGGCTGGATATGGCCCCATTGAGCAGCTCCCAGA
  CTACAACCGGATCCGTAGCGGCATGTTCTGGCTGCGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203299 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASVVCRAATAGAQVLLRARRSPALLRTPALRSTATFAQALQFVPETQVSLLDNGLRVASEQSSQPTCT
  VGVWIDVGSRFETEKNNGAGYFLEHLAFKGTKNRPGSALEKEVESMGAHLNAYSTREHTAYYIKALSKDL
  PKAVELLGDIVQNCSLEDSQIEKERDVILREMQENDASMRDVVFNYLHATAFQGTPLAQAVEGPSENVRK
  LSRADLTEYLSTHYKAPRMVLAAAGGVEHQQLLDLAQKHLGGIPWTYAEDAVPTLTPCRFTGSEIRHRDD
  ALPFAHVAIAVEGPGWASPDNVALQVANAIIGHYDCTYGGGVHLSSPLASGAVANKLCQSFQTFSICYAE
  TGLLGAHFVCDRMKIDDMMFVLQGQWMRLCTSATESEVARGKNILRNALVSHLDGTTPVCEDIGRSLLTY
  GRRIPLAEWESRIAEVDASVVREICSKYIYDQCPAVAGYGPIEQLPDYNRIRSGMFWLRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003365
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003365.3
• RefSeq Size: 1636 bp
• RefSeq ORF: 1443 bp
• Locus ID: 7384
• UniProt ID: P31930
• Cytogenetics: 3p21.31
• Domains: Peptidase_M16, Peptidase_M16_C
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• MW: 52.6 kDa
• Gene Summary: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.[UniProtKB/Swiss-Prot Function]