CHPF (NM_024536) Human Tagged Lenti ORF Clone

SKU
RC203197L3
Lenti ORF clone of Human chondroitin polymerizing factor (CHPF), transcript variant 1, Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$1,010.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CHPF
Synonyms CHSY2; CSS2
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC203197).
Restriction Sites SgfI-MluI Cloning Scheme for this gene
ACCN NM_024536
ORF Size 2325 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024536.4, NP_078812.2
RefSeq Size 3013 bp
RefSeq ORF 2328 bp
Locus ID 79586
UniProt ID Q8IZ52
Cytogenetics 2q35
Protein Families Druggable Genome
Protein Pathways Chondroitin sulfate biosynthesis, Metabolic pathways
MW 85.5 kDa
Summary Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Isoform 2 may facilitate PRKN transport into the mitochondria. In collaboration with PRKN, isoform 2 may enhance cell viability and protect cells from oxidative stress.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC203197 CHPF (Myc-DDK-tagged)-Human chondroitin polymerizing factor (CHPF), transcript variant 1 10 ug
$710.00
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RG203197 CHPF (tGFP-tagged) - Human chondroitin polymerizing factor (CHPF), transcript variant 1 10 ug
$789.00 MSRP $910.00 MSRP $910.00
SC127898 CHPF (untagged)-Human chondroitin polymerizing factor (CHPF), transcript variant 1 10 ug
$711.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.