LETM1 (NM_012318) Human Tagged ORF Clone

CAT#: RC202855

LETM1 (Myc-DDK-tagged)-Human leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC202855

• 

  Western blot validation of overexpression lysate (Cat# LY402195) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202855 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LETM1
• Synonyms: SLC55A1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202855 representing NM_012318
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCATCTTACTGAGGAGCTGCCGCGGCCGGGCGCCCGCCCGCCTCCCGCCGCCGCCTCGGTACA
  CCGTCCCGCGGGGTAGTCCAGGGGATCCTGCTCATCTCAGCTGTGCCAGCACCCTGGGGTTGAGGAACTG
  CCTGAATGTTCCATTTGGCTGCTGCACTCCCATCCACCCTGTGTACACATCCTCCAGAGGCGATCACCTC
  GGCTGTTGGGCTCTGAGGCCCGAGTGCCTTCGCATAGTGTCGAGAGCGCCATGGACCTCTACCTCTGTGG
  GTTTTGTGGCTGTGGGACCTCAGTGCCTTCCTGTGCGTGGCTGGCACTCTTCGCGCCCTGTTCGCGATGA
  CTCGGTAGTAGAGAAGTCCCTCAAGTCCTTGAAGGACAAGAACAAGAAGCTGGAGGAAGGCGGCCCGGTG
  TACAGCCCCCCCGCAGAGGTGGTGGTGAAGAAGTCCCTGGGGCAGCGGGTGCTGGACGAGCTGAAGCACT
  ACTACCATGGCTTCCGCCTGCTATGGATCGACACCAAGATCGCGGCACGCATGCTCTGGCGCATCCTCAA
  CGGCCACAGCCTGACCCGCCGGGAGCGCAGGCAGTTTCTCCGGATCTGCGCTGACCTCTTCCGCCTGGTG
  CCGTTCCTTGTGTTCGTGGTGGTGCCGTTCATGGAGTTTCTGCTGCCTGTTGCTGTGAAGCTCTTCCCCA
  ACATGTTGCCATCCACATTTGAGACTCAGTCACTCAAGGAGGAGAGGCTGAAGAAGGAGCTTCGGGTCAA
  GCTGGAGCTGGCCAAGTTCCTCCAGGACACCATCGAGGAGATGGCCTTGAAGAACAAGGCAGCCAAGGGC
  AGCGCCACCAAAGACTTCTCTGTGTTTTTCCAGAAGATCCGGGAAACAGGGGAGAGGCCCAGCAATGAGG
  AAATCATGCGTTTTTCCAAATTATTTGAGGATGAGCTGACCCTGGACAACCTGACACGGCCGCAGCTGGT
  GGCCCTGTGCAAGCTGCTGGAGCTACAGTCCATCGGCACCAACAACTTCCTGCGCTTCCAGCTTACCATG
  CGGCTGCGCTCCATAAAGGCAGACGACAAGCTGATTGCTGAGGAAGGGGTGGACAGCCTGAATGTCAAGG
  AGCTGCAGGCAGCGTGTCGGGCACGAGGCATGCGGGCCCTGGGCGTCACGGAAGACCGCCTGAGGGGTCA
  GCTGAAGCAGTGGCTGGACCTGCACCTGCATCAGGAGATCCCCACATCGCTGCTCATCCTGTCCCGGGCC
  ATGTACCTCCCGGACACCCTCTCTCCAGCCGACCAGCTCAAGTCCACACTGCAGACCCTCCCAGAGATTG
  TGGCAAAGGAAGCACAGGTGAAAGTGGCCGAGGTGGAGGGCGAGCAGGTGGACAACAAGGCCAAGCTGGA
  GGCCACGCTGCAGGAGGAGGCGGCCATCCAGCAGGAGCACCGTGAGAAGGAGCTGCAGAAGCGCTCGGAG
  GTGGCGAAGGATTTTGAGCCCGAACGTGTGGTAGCTGCTCCCCAAAGGCCGGGGACCGAGCCACAGCCAG
  AAATGCCTGACACAGTCCTGCAGTCAGAGACCTTGAAGGACACTGCCCCGGTGCTGGAGGGCTTGAAGGA
  GGAAGAGATCACGAAGGAGGAAATCGACATCCTCAGCGATGCCTGCTCTAAGCTGCAGGAGCAGAAGAAG
  TCACTCACCAAGGAGAAGGAGGAGCTGGAGCTGCTGAAGGAGGATGTGCAGGACTACAGCGAGGACTTGC
  AGGAGATCAAGAAGGAACTTTCAAAGACTGGTGAAGAAAAATACGTGGAAGAATCTAAAGCCAGCAAGAG
  ATTGACAAAAAGGGTGCAGCAAATGATCGGGCAGATCGATGGCTTGATCTCGCAGCTGGAGATGGACCAG
  CAGGCTGGCAAGCTGGCCCCGGCCAACGGCATGCCCACGGGGGAGAACGTCATCAGTGTCGCTGAGCTCA
  TCAACGCCATGAAGCAAGTCAAGCACATTCCCGAAAGCAAGCTCACCAGCCTGGCCGCAGCACTGGATGA
  AAACAAGGATGGCAAGGTCAACATCGACGACCTCGTCAAGGTGATTGAGCTGGTGGACAAAGAAGATGTT
  CACATCTCCACCAGCCAGGTGGCTGAGATTGTAGCAACACTGGAAAAAGAGGAGAAGGTGGAGGAGAAGG
  AGAAGGCCAAAGAGAAGGCAGAGAAGGAGGTCGCAGAGGTGAAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202855 representing NM_012318
  Red=Cloning site Green=Tags(s)
  
  MASILLRSCRGRAPARLPPPPRYTVPRGSPGDPAHLSCASTLGLRNCLNVPFGCCTPIHPVYTSSRGDHL
  GCWALRPECLRIVSRAPWTSTSVGFVAVGPQCLPVRGWHSSRPVRDDSVVEKSLKSLKDKNKKLEEGGPV
  YSPPAEVVVKKSLGQRVLDELKHYYHGFRLLWIDTKIAARMLWRILNGHSLTRRERRQFLRICADLFRLV
  PFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSLKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKG
  SATKDFSVFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTM
  RLRSIKADDKLIAEEGVDSLNVKELQAACRARGMRALGVTEDRLRGQLKQWLDLHLHQEIPTSLLILSRA
  MYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQKRSE
  VAKDFEPERVVAAPQRPGTEPQPEMPDTVLQSETLKDTAPVLEGLKEEEITKEEIDILSDACSKLQEQKK
  SLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYVEESKASKRLTKRVQQMIGQIDGLISQLEMDQ
  QAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDV
  HISTSQVAEIVATLEKEEKVEEKEKAKEKAEKEVAEVKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012318
• ORF Size: 2217 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012318.1, NP_036450.1
• RefSeq Size: 3907 bp
• RefSeq ORF: 2220 bp
• Locus ID: 3954
• UniProt ID: O95202
• Cytogenetics: 4p16.3
• Protein Families: Transmembrane
• MW: 83.35 kDa
• Gene Summary: This gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause Wolf-Hirschhorn syndrome, a complex malformation syndrome caused by the deletion of parts of the distal short arm of chromosome 4. Related pseudogenes have been identified on chromosomes 8, 15 and 19. [provided by RefSeq, Oct 2009]