LETM1 (NM_012318) Human Untagged Clone

CAT#: SC320328

LETM1 (untagged)-Human leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LETM1
• Synonyms: SLC55A1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012318.1 GCGGCCTGGCCGCCCCGCCCCGCGCCTCCGGACGTCTGTGCCGGGACAAGGCCGCCGCTG
  GTGCCGGGTCCTTGAGGAGAGCGCCTCCCGTCCGAGGCCAGCCGCCTCTGTCAGCCGTCC
  GCGCGGGCCGGGTCTGAAGCGCCGCCGGGACGGCGAAGAGCCGCGGCCGCCGCGGAGAAG
  GAGGCAGCGCAGGAGGCCGGAGCGGCCGCCGCGCCCGAGCACATGGCGTCCATCTTACTG
  AGGAGCTGCCGCGGCCGGGCGCCCGCCCGCCTCCCGCCGCCGCCTCGGTACACCGTCCCG
  CGGGGTAGTCCAGGGGATCCTGCTCATCTCAGCTGTGCCAGCACCCTGGGGTTGAGGAAC
  TGCCTGAATGTTCCATTTGGCTGCTGCACTCCCATCCACCCTGTGTACACATCCTCCAGA
  GGCGATCACCTCGGCTGTTGGGCTCTGAGGCCCGAGTGCCTTCGCATAGTGTCGAGAGCG
  CCATGGACCTCTACCTCTGTGGGTTTTGTGGCTGTGGGACCTCAGTGCCTTCCTGTGCGT
  GGCTGGCACTCTTCGCGCCCTGTTCGCGATGACTCGGTAGTAGAGAAGTCCCTCAAGTCC
  TTGAAGGACAAGAACAAGAAGCTGGAGGAAGGCGGCCCGGTGTACAGCCCCCCCGCAGAG
  GTGGTGGTGAAGAAGTCCCTGGGGCAGCGGGTGCTGGACGAGCTGAAGCACTACTACCAT
  GGCTTCCGCCTGCTATGGATCGACACCAAGATCGCGGCACGCATGCTCTGGCGCATCCTC
  AACGGCCACAGCCTGACCCGCCGGGAGCGCAGGCAGTTTCTCCGGATCTGCGCTGACCTC
  TTCCGCCTGGTGCCGTTCCTTGTGTTCGTGGTGGTGCCGTTCATGGAGTTTCTGCTGCCT
  GTTGCTGTGAAGCTCTTCCCCAACATGTTGCCATCCACATTTGAGACTCAGTCACTCAAG
  GAGGAGAGGCTGAAGAAGGAGCTTCGGGTCAAGCTGGAGCTGGCCAAGTTCCTCCAGGAC
  ACCATCGAGGAGATGGCCTTGAAGAACAAGGCAGCCAAGGGCAGCGCCACCAAAGACTTC
  TCTGTGTTTTTCCAGAAGATCCGGGAAACAGGGGAGAGGCCCAGCAATGAGGAAATCATG
  CGTTTTTCCAAATTATTTGAGGATGAGCTGACCCTGGACAACCTGACACGGCCGCAGCTG
  GTGGCCCTGTGCAAGCTGCTGGAGCTACAGTCCATCGGCACCAACAACTTCCTGCGCTTC
  CAGCTTACCATGCGGCTGCGCTCCATAAAGGCAGACGACAAGCTGATTGCTGAGGAAGGG
  GTGGACAGCCTGAATGTCAAGGAGCTGCAGGCAGCGTGTCGGGCACGAGGCATGCGGGCC
  CTGGGCGTCACGGAAGACCGCCTGAGGGGTCAGCTGAAGCAGTGGCTGGACCTGCACCTG
  CATCAGGAGATCCCCACATCGCTGCTCATCCTGTCCCGGGCCATGTACCTCCCGGACACC
  CTCTCTCCAGCCGACCAGCTCAAGTCCACACTGCAGACCCTCCCAGAGATTGTGGCAAAG
  GAAGCACAGGTGAAAGTGGCCGAGGTGGAGGGCGAGCAGGTGGACAACAAGGCCAAGCTG
  GAGGCCACGCTGCAGGAGGAGGCGGCCATCCAGCAGGAGCACCGTGAGAAGGAGCTGCAG
  AAGCGCTCGGAGGTGGCGAAGGATTTTGAGCCCGAACGTGTGGTAGCTGCTCCCCAAAGG
  CCGGGGACCGAGCCACAGCCAGAAATGCCTGACACAGTCCTGCAGTCAGAGACCTTGAAG
  GACACTGCCCCGGTGCTGGAGGGCTTGAAGGAGGAAGAGATCACGAAGGAGGAAATCGAC
  ATCCTCAGCGATGCCTGCTCTAAGCTGCAGGAGCAGAAGAAGTCACTCACCAAGGAGAAG
  GAGGAGCTGGAGCTGCTGAAGGAGGATGTGCAGGACTACAGCGAGGACTTGCAGGAGATC
  AAGAAGGAACTTTCAAAGACTGGTGAAGAAAAATACGTGGAAGAATCTAAAGCCAGCAAG
  AGATTGACAAAAAGGGTGCAGCAAATGATCGGGCAGATCGATGGCTTGATCTCGCAGCTG
  GAGATGGACCAGCAGGCTGGCAAGCTGGCCCCGGCCAACGGCATGCCCACGGGGGAGAAC
  GTCATCAGTGTCGCTGAGCTCATCAACGCCATGAAGCAAGTCAAGCACATTCCCGAAAGC
  AAGCTCACCAGCCTGGCCGCAGCACTGGATGAAAACAAGGATGGCAAGGTCAACATCGAC
  GACCTCGTCAAGGTGATTGAGCTGGTGGACAAAGAAGATGTTCACATCTCCACCAGCCAG
  GTGGCTGAGATTGTAGCAACACTGGAAAAAGAGGAGAAGGTGGAGGAGAAGGAGAAGGCC
  AAAGAGAAGGCAGAGAAGGAGGTCGCAGAGGTGAAGAGCTAGAACCACTGGCCTGGGCAC
  CTGTCCTCCTGCTGTGCCGTCACCCTGGCAAGGGCCGTGAGGGCGATTGCTTTGTGGTGA
  TTCTCAGTGGCTCATCTAATATTTTGGCTGGAATAAATCAGAGACTTCCATAATCAAGTA
  AATTTTAATTTTCATCATTCCATGGAGATTCAGTCTGTCTGGAATCCCGGAACCCCTCCA
  CAGAATCGTGTCTGGATCCACACTGTGGTGTGGCTGCCACGGCCTCCTGGCTCCAGAGGC
  AGCTGGGCCGGGCCAAGGCAGGAAGGCGCCCCCATGTGTGTGGCCTCAGTCTCAGCATCT
  GACTGTGCTGCCCTGTCCCCAGGGAAGAGAATGAGGACCACGTGGACTCCGCCCACACAG
  AGCTGGCTGCTGTGCCCACCCTCAGGGGCGTCAGGAGACACAGCCTGGCCTCCTCAGGGC
  TGAAGATGCCTCCATTCGCCTGTGGACCTTGATTTCTAGATTTCAGTGTCAATAGACCTG
  TCTTCCTGCACACTTTCAGTTGGATGTGGGTCATTGTGGAGACAGAGGTGTCTGCCATCT
  CTGTGGCCTCTGGAGAGTGACGTCTCCCTGCTGGGAGTGCTGGTTCTCACGTGCGGTTTC
  CCTTGTGTATCGGAGCTCTTTCTCGGCTTTCTATTTCCCCCAGTTCTTTAAGCAGTCAAT
  TGGCACAGAGTTTCCCACGGGGGCTGCAGTGGATTCACTGTGTCAGGGATGAGCCTGGCT
  TGGGGGTTGGTGGGGCTCTGAAGCGCATTTGGGGTTCTTTGTAGCTTCTAATGTGAGGTT
  TGAGTCCAGTGCGCCCACAGCAGCATGCCCTTCTCACCCCTTGCAGTGTTGCAGGCTCAG
  GCCCCTGGGCTCCTTCAGCAAGCAGGCTAGTCAATGAGGCATGAGGATCCGGCACAGGGC
  ATCCCCTGGGGCTTCAAGGGCAATACCCCCGTGCTTAGGGTTTGCCCTGTGCCCTCTGGG
  TGGGGTGGCCTCCCCGCACTCGGCAGCATGGCCAGGCTGGCCAGGGCGTGGGCAGGTGGT
  GTCCTGTGGCACCTCCATCCTCCTGCCCAGCCGGCTGTGTCACACTCATCTTTTTAAGGT
  CAGGTTGGTTCCTGGCAAAAATGTACCTCCAGGGGCCTCCAAGCATAGGATTTGGAAGAC
  AGGAACGGCACAGGCGTCCAGGAAAGCAGCTGCACTCAGACAATGCCTTCTCCATTACTT
  GAAGCTTCTTTCTGTTCAGCCATTAAAGAAACTTGACAAAATAGGAACAGGAGATATTTC
  TCAATTGTAAACCTTTGTGCAGGGACAGTTGGCTTCCAGAGGCTTTCAGCTTTCAGTTAT
  TTGAGAAGTTTGTTTTAGATCCTTAACTAATATTTATGAATTCTCTCAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_012318
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012318.1, NP_036450.1
• RefSeq Size: 3907 bp
• RefSeq ORF: 2220 bp
• Locus ID: 3954
• UniProt ID: O95202
• Cytogenetics: 4p16.3
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause Wolf-Hirschhorn syndrome, a complex malformation syndrome caused by the deletion of parts of the distal short arm of chromosome 4. Related pseudogenes have been identified on chromosomes 8, 15 and 19. [provided by RefSeq, Oct 2009]