LETM1 (NM_012318) Human Untagged Clone

SKU SC115448

LETM1 (untagged)-Human leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: LETM1
• Synonyms: SLC55A1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115448 sequence for NM_012318 edited (data generated by NextGen Sequencing); ATGGCGTCCATCTTACTGAGGAGCTGCCGCGGCCGGGCGCCCGCCCGCCTCCCGCCGCCG; CCTCGGTACACCGTCCCGCGGGGTAGTCCAGGGGATCCTGCTCATCTCAGCTGTGCCAGC; ACCCTGGGGTTGAGGAACTGCCTGAATGTTCCATTTGGCTGCTGCACTCCCATCCACCCT; GTGTACACATCCTCCAGAGGCGATCACCTCGGCTGTTGGGCTCTGAGGCCCGAGTGCCTT; CGCATAGTGTCGAGAGCGCCATGGACCTCTACCTCTGTGGGTTTTGTGGCTGTGGGACCT; CAGTGCCTTCCTGTGCGTGGCTGGCACTCTTCGCGCCCTGTTCGCGATGACTCGGTAGTA; GAGAAGTCCCTCAAGTCCTTGAAGGACAAGAACAAGAAGCTGGAGGAAGGCGGCCCGGTG; TACAGCCCCCCCGCAGAGGTGGTGGTGAAGAAGTCCCTGGGGCAGCGGGTGCTGGACGAG; CTGAAGCACTACTACCATGGCTTCCGCCTGCTATGGATCGACACCAAGATCGCGGCACGC; ATGCTCTGGCGCATCCTCAACGGCCACAGCCTGACCCGCCGGGAGCGCAGGCAGTTTCTC; CGGATCTGCGCTGACCTCTTCCGCCTGGTGCCGTTCCTTGTGTTCGTGGTGGTGCCGTTC; ATGGAGTTTCTGCTGCCTGTTGCTGTGAAGCTCTTCCCCAACATGTTGCCATCCACATTT; GAGACTCAGTCACTCAAGGAGGAGAGGCTGAAGAAGGAGCTTCGGGTCAAGCTGGAGCTG; GCCAAGTTCCTCCAGGACACCATCGAGGAGATGGCCTTGAAGAACAAGGCAGCCAAGGGC; AGCGCCACCAAAGACTTCTCTGTGTTTTTCCAGAAGATCCGGGAAACAGGGGAGAGGCCC; AGCAATGAGGAAATCATGCGTTTTTCCAAATTATTTGAGGATGAGCTGACCCTGGACAAC; CTGACACGGCCGCAGCTGGTGGCCCTGTGCAAGCTGCTGGAGCTACAGTCCATCGGCACC; AACAACTTCCTGCGCTTCCAGCTTACCATGCGGCTGCGCTCCATAAAGGCAGACGACAAG; CTGATTGCTGAGGAAGGGGTGGACAGCCTGAATGTCAAGGAGCTGCAGGCAGCGTGTCGG; GCACGAGGCATGCGGGCCCTGGGCGTCACGGAAGACCGCCTGAGGGGTCAGCTGAAGCAG; TGGCTGGACCTGCACCTGCATCAGGAGATCCCCACATCGCTGCTCATCCTGTCCCGGGCC; ATGTACCTCCCGGACACCCTCTCTCCAGCCGACCAGCTCAAGTCCACACTGCAGACCCTC; CCAGAGATTGTGGCAAAGGAAGCACAGGTGAAAGTGGCCGAGGTGGAGGGCGAGCAGGTG; GACAACAAGGCCAAGCTGGAGGCCACGCTGCAGGAGGAGGCGGCCATCCAGCAGGAGCAC; CGTGAGAAGGAGCTGCAGAAGCGCTCGGAGGTGGCGAAGGATTTTGAGCCCGAACGTGTG; GTAGCTGCTCCCCAAAGGCCGGGGACCGAGCCACAGCCAGAAATGCCTGACACAGTCCTG; CAGTCAGAGACCTTGAAGGACACTGCCCCGGTGCTGGAGGGCTTGAAGGAGGAAGAGATC; ACGAAGGAGGAAATCGACATCCTCAGCGATGCCTGCTCTAAGCTGCAGGAGCAGAAGAAG; TCACTCACCAAGGAGAAGGAGGAGCTGGAGCTGCTGAAGGAGGATGTGCAGGACTACAGC; GAGGACTTGCAGGAGATCAAGAAGGAACTTTCAAAGACTGGTGAAGAAAAATACGTGGAA; GAATCTAAAGCCAGCAAGAGATTGACAAAAAGGGTGCAGCAAATGATCGGGCAGATCGAT; GGCTTGATCTCGCAGCTGGAGATGGACCAGCAGGCTGGCAAGCTGGCCCCGGCCAACGGC; ATGCCCACGGGGGAGAACGTCATCAGTGTCGCTGAGCTCATCAACGCCATGAAGCAAGTC; AAGCACATTCCCGAAAGCAAGCTCACCAGCCTGGCCGCAGCACTGGATGAAAACAAGGAT; GGCAAGGTCAACATCGACGACCTCGTCAAGGTGATTGAGCTGGTGGACAAAGAAGATGTT; CACATCTCCACCAGCCAGGTGGCTGAGATTGTAGCAACACTGGAAAAAGAGGAGAAGGTG; GAGGAGAAGGAGAAGGCCAAAGAGAAGGCAGAGAAGGAGGTCGCAGAGGTGAAGAGCTAG; ; Clone variation with respect to NM_012318.2; 1791 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012318 unedited; NGGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCGCTGA; GGGGCCCAATGGGAGCCGCGGCCTGGCCGCCCCGCCCCGCGCCTCCGGACGTCTGTGCCG; GGACAAGGCCGCCGCTGGTGCCGGGTCCTTGAGGAGAGCGCCTCCCGTCCGAGGCCAGCC; GCCTCTGTCAGCCGTCCGCGCGGGCCGGGTCTGAAGCGCCGCCGGGACGGCGAAGAGCCG; CGGCCGCCGCGGAGAAGGAGGCAGCGCAGGAGGCCGGAGCGGCCGCCGCGCCCGAGCACA; TGGCGTCCATCTTACTGAGGAGCTGCCGCGGCCGGGCGCCCGCCCGCCTCCCGCCGCCGC; CTCGGTACACCGTCCCGCGGGGTAGTCCAGGGGATCCTGCTCATCTCAGCTGTGCCAGCA; CCCTGGGGTTGAGGAACTGCCTGAATGTTCCATTTGGCTGCTGCACTCCCATCCACCCTG; TGTACACATCCTCCAGAGGCGATCACCTCGGCTGTTGGGCTCTGAGGCCCGAGTGCCTTC; GCATAGTGTCGAGAGCGCCATGGACCTCTACCTCTGTGGNGTTTTGTGGNCTGTGGGACC; TCAGTGCCTTCCTGTGCGTGGCTGGCACTCTTCGCGCCCTGTTCGCGATGACTCGGTAGT; AAAAGAAGTCCCTCAAGTCCTTGAAGGACAAGAACAAGAAGCTGGAGGAAGGCGGCCCCG; GTGTACAGCCCCCCCGCANAGGTGGGTGGTGGAAGAAGTCCCCTGGGCCAACCGGGTGCT; TGGACGANCTGAAGCCCTACTACCATGGCTTCCGCCTGTTATGGATCGACACCAAGATCG; CGGTACGCATGCTTTGGCGCATCCTCAACGGGCACAGCCTGACCCGCCGGNAGCCGCCAG; CAGTTCTCCCGGATCTGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_012318
• Insert Size: 4470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012318.1, NP_036450.1
• RefSeq Size: 3907 bp
• RefSeq ORF: 2220 bp
• Locus ID: 3954
• UniProt ID: O95202
• Cytogenetics: 4p16.3
• Protein Families: Transmembrane
• Summary: This gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause Wolf-Hirschhorn syndrome, a complex malformation syndrome caused by the deletion of parts of the distal short arm of chromosome 4. Related pseudogenes have been identified on chromosomes 8, 15 and 19. [provided by RefSeq, Oct 2009]