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DDIT4 (NM_019058) Human Tagged ORF Clone

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SKU
RC202847
DDIT4 (Myc-DDK-tagged)-Human DNA-damage-inducible transcript 4 (DDIT4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "DDIT4 or NM_019058"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DDIT4
Synonyms Dig2; REDD-1; REDD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202847 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTAGCCTTTGGGACCGCTTCTCGTCGTCGTCCACCTCCTCTTCGCCCTCGTCCTTGCCCCGAACTC
CCACCCCAGATCGGCCGCCGCGCTCAGCCTGGGGGTCGGCGACCCGGGAGGAGGGGTTTGACCGCTCCAC
GAGCCTGGAGAGCTCGGACTGCGAGTCCCTGGACAGCAGCAACAGTGGCTTCGGGCCGGAGGAAGACACG
GCTTACCTGGATGGGGTGTCGTTGCCCGACTTCGAGCTGCTCAGTGACCCTGAGGATGAACACTTGTGTG
CCAACCTGATGCAGCTGCTGCAGGAGAGCCTGGCCCAGGCGCGGCTGGGCTCTCGACGCCCTGCGCGCCT
GCTGATGCCTAGCCAGTTGGTAAGCCAGGTGGGCAAAGAACTACTGCGCCTGGCCTACAGCGAGCCGTGC
GGCCTGCGGGGGGCGCTGCTGGACGTCTGCGTGGAGCAGGGCAAGAGCTGCCACAGCGTGGGCCAGCTGG
CACTCGACCCCAGCCTGGTGCCCACCTTCCAGCTGACCCTCGTGCTGCGCCTGGACTCACGACTCTGGCC
CAAGATCCAGGGGCTGTTTAGCTCCGCCAACTCTCCCTTCCTCCCTGGCTTCAGCCAGTCCCTGACGCTG
AGCACTGGCTTCCGAGTCATCAAGAAGAAGCTGTACAGCTCGGAACAGCTGCTCATTGAGGAGTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202847 protein sequence
Red=Cloning site Green=Tags(s)

MPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDT
AYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPC
GLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTL
STGFRVIKKKLYSSEQLLIEEC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019058
ORF Size 696 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019058.4
RefSeq Size 1752 bp
RefSeq ORF 699 bp
Locus ID 54541
UniProt ID Q9NX09
Cytogenetics 10q22.1
Protein Pathways mTOR signaling pathway
MW 25.4 kDa
Summary Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.UniProtKB/Swiss-Prot Function
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"NM_019058" in other vectors (6)
SKU Description Size Price
RC202847L1 Lenti ORF clone of Human DNA-damage-inducible transcript 4 (DDIT4), Myc-DDK-tagged 10 ug
$600.00
RC202847L2 Lenti ORF clone of Human DNA-damage-inducible transcript 4 (DDIT4), mGFP tagged 10 ug
$600.00
RC202847L3 Lenti ORF clone of Human DNA-damage-inducible transcript 4 (DDIT4), Myc-DDK-tagged 10 ug
$600.00
RC202847L4 Lenti ORF clone of Human DNA-damage-inducible transcript 4 (DDIT4), mGFP tagged 10 ug
$600.00
RG202847 DDIT4 (tGFP-tagged) - Human DNA-damage-inducible transcript 4 (DDIT4) 10 ug
$489.00 $500.00
SC128247 DDIT4 (untagged)-Human DNA-damage-inducible transcript 4 (DDIT4) 10 ug
$450.00

Plasmid Map for RC202847

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202847
Circular map for RC202847

file-alt Citations

Histone deacetylase 4 reverses cellular senescence via DDIT4 in dermal fibroblasts
Lee, Song, Park et al
Aging (Albany NY) (2022) 14 (11), 4653-4672 DOI: 10.18632/aging.204118
Cited by 21
AI Summary: OriGene contributed to this study by providing the DDIT4 mammalian expression plasmid (RC202847), which was used to investigate the role of DDIT4 in reversing cellular senescence in human dermal fibroblasts.
Snippet: … Plasmid constructs, transfection, and lentivirus production The mammalian expression plasmids for HDAC4 (plasmid #30485) and DDIT4 (RC202847) were purchased from Addgene (Cambridge, MA, USA) and OriGene (Rockville, MD, USA), respectively. … HDAC4 lentiviral parti;The mammalian expression plasmids for HDAC4 (plasmid #30485) and DDIT4 (RC202847) were purchased from Addgene (Cambridge, MA, USA) and OriGene (Rockville, MD, USA), respectively
APOBEC3G-regulated host factors interfere with measles virus replication: role of REDD1 and mTORC1 inhibition
Tiwarekar, Wohlfahrt, Fehrholz et al
J. Virol. (2018)
AI Summary: OriGene’s REDD1 expression plasmid (RC202847) was used to generate a lentiviral vector for REDD1 overexpression, enabling the authors to demonstrate that increased REDD1 inhibits measles virus replication by modulating mTORC1 activity.
Snippet: Lentiviral expression vector for REDD1 (F6gW-REDD1-Myc-DDK-tag) 347 was generated using the BamH1-SacII fragment of REDD1-expression plasmid RC202847 348 (OriGene) and cloned into the BamH1-EcoR1 sites of F6gW. Vero cells were transduced with
Showing 1-2 of 2 papers.
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