PAR4 (PAWR) (NM_002583) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PAR4 |
| Synonyms | Par-4; PAR4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002583 |
| ORF Size | 1020 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002583.4 |
| RefSeq Size | 1967 bp |
| RefSeq ORF | 1023 bp |
| Locus ID | 5074 |
| UniProt ID | Q96IZ0 |
| Cytogenetics | 12q21.2 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 36.6 kDa |
| Summary | This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002583" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202733L1 | Lenti ORF clone of Human PRKC, apoptosis, WT1, regulator (PAWR), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC202733L2 | Lenti ORF clone of Human PRKC, apoptosis, WT1, regulator (PAWR), mGFP tagged | 10 ug |
$757.00
|
|
| RC202733L3 | Lenti ORF clone of Human PRKC, apoptosis, WT1, regulator (PAWR), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC202733L4 | Lenti ORF clone of Human PRKC, apoptosis, WT1, regulator (PAWR), mGFP tagged | 10 ug |
$757.00
|
|
| RG202733 | PAWR (tGFP-tagged) - Human PRKC, apoptosis, WT1, regulator (PAWR) | 10 ug |
$489.00
$657.00
|
|
| SC110969 | PAWR (untagged)-Human PRKC, apoptosis, WT1, regulator (PAWR) | 10 ug |
$457.00
|
|
| SC320836 | PAWR (untagged)-Human PRKC, apoptosis, WT1, regulator (PAWR) | 10 ug |
$457.00
|
|
Citations
Par-4 regulates autophagic cell death in human cancer cells via upregulating p53 and BNIP3
Thayyullathil, Cheratta, Pallichankandy et al
Biochim Biophys Acta Mol Cell Res (2020) 1867 (7), 118692 DOI: 10.1016/j.bbamcr.2020.118692
Thayyullathil, Cheratta, Pallichankandy et al
Biochim Biophys Acta Mol Cell Res (2020) 1867 (7), 118692 DOI: 10.1016/j.bbamcr.2020.118692
Cited by 18
AI Summary: In this study, OriGene’s Par-4 expression plasmid (RC207307) and Par-4 shRNA plasmid (TF314894) were used to modulate Par-4 levels in human cancer cells, enabling the authors to demonstrate that Par-4 regulates autophagic cell death via upregulation of p53 and BNIP3.
Critical role of H2O2 in mediating sanguinarine-induced apoptosis in prostate cancer cells via facilitating ceramide generation, ERK1/2 phosphorylation, and Par-4 cleavage
Rahman, Pallichankandy, Thayyullathil et al
Free Radic. Biol. Med. (2019) 134, 527-544 DOI: 10.1016/j.freeradbiomed.2019.01.039
Rahman, Pallichankandy, Thayyullathil et al
Free Radic. Biol. Med. (2019) 134, 527-544 DOI: 10.1016/j.freeradbiomed.2019.01.039
AI Summary: In this study, OriGene Technologies' anti-DDK antibody and Mega Trans 1.0 transfection reagent were used to facilitate protein detection and gene transfection experiments, contributing to the investigation of sanguinarine-induced apoptosis mechanisms in prostate cancer cells.
Snippet: USA). Anti-caspase-9, anti-caspase-8, anti-caspase-3, anti-PARP, and anti-phospho ERK1/2 (T202/Y204) antibodies were from Cell Signaling Technology (Beverly, MA, USA). Oxidation sensitive DCFH-DA and C6-NBD-Cer were purchased from Molecular Probes (Eugene, OR, USA). N-Oleoyl-ethanolamine (NOE) was from Matreya (PA, USA) and D-erythro-C12-NBD-Cer was a kind gift from Prof. YA Hannun (Department of ...
Par-4-dependent p53 up-regulation plays a critical role in thymoquinone-induced cellular senescence in human malignant glioma cells
Subburayan, Thayyullathil, Pallichankandy et al
Cancer Lett. (2018) 426, 80-97 DOI: 10.1016/j.canlet.2018.04.009
Subburayan, Thayyullathil, Pallichankandy et al
Cancer Lett. (2018) 426, 80-97 DOI: 10.1016/j.canlet.2018.04.009
AI Summary: OriGene's pCMV6-Myc-DDK-tagged Par-4 plasmid (RC202733) was used to overexpress Par-4 in glioma cells, enabling the study of Par-4's role in thymoquinone-induced cellular senescence.
Snippet: Gel Doc XR System (Bio-Rad). 2.9. Plasmids and transient transfection. pCMV6-Myc-DDK-tagged Par-4 (RC202733) was purchased from OriGene Technologies Inc., (Rockville, MD, USA). DNA transfection of cells was performed
TRIM21 is a novel regulator of Par-4 in colon and pancreatic cancer cells
Nguyen, Irby
Cancer Biol. Ther. (2016)
Nguyen, Irby
Cancer Biol. Ther. (2016)
AI Summary: OriGene products, specifically the Par-4 plasmid and anti-TRIM21 shRNA plasmids, were used in this study to manipulate the expression of Par-4 and TRIM21, enabling the authors to investigate the regulatory relationship between these proteins in colon and pancreatic cancer cells.
Snippet: The Par-4 plasmid and anti-TRIM21 shRNA plasmids were obtained from Origene Technologies (Rockville, MD)
ROS-dependent prostate apoptosis response-4 (Par-4) up-regulation and ceramide generation are the prime signaling events associated with curcumin-induced autophagic cell death in human malignant glioma
Thayyullathil, Rahman, Pallichankandy et al
FEBS Open Bio (2014) 4, 763-76 DOI: 10.1016/j.fob.2014.08.005
Thayyullathil, Rahman, Pallichankandy et al
FEBS Open Bio (2014) 4, 763-76 DOI: 10.1016/j.fob.2014.08.005
Cited by 63
AI Summary: In this study, OriGene’s Par-4 expression plasmid (RC200377) and Par-4 shRNA (TF313814) were used to manipulate Par-4 levels in glioma cells, enabling the authors to demonstrate that Par-4 upregulation is a key mediator of curcumin-induced autophagic cell death.
ROS-dependent prostate apoptosis response-4 (Par-4) up-regulation and ceramide generation are the prime signaling events associated with curcumin-induced autophagic cell death in human malignant glioma
Thayyullathil, Rahman, Pallichankandy et al
FEBS Open Bio (2014) 4, 763–776
Thayyullathil, Rahman, Pallichankandy et al
FEBS Open Bio (2014) 4, 763–776
AI Summary: In this study, OriGene products—including MegaTran 1.0 transfection reagent, pCMV6-XL6-Par-4 plasmid (SC110969), and pCMV6-Myc-DDK-tagged PAR-4 plasmid (RC202733)—were used to facilitate gene transfection and Par-4 overexpression, enabling the investigation of curcumin-induced autophagic cell death mechanisms in human malignant glioma cells.
Showing 1-6 of 10 papers.
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