UBE2H (NM_003344) Human Tagged ORF Clone
SKU
RC202516
UBE2H (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1
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TrueORF Gold
Protein expression verified by Western blot, fully sequenced and in stock
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Product Data | |
Target Symbol | UBE2H |
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Synonyms | E2-20K; GID3; UBC8; UBCH; UBCH2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC202516 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTCCCAGTCCGGGCAAGAGGCGGATGGACACGGACGTGGTCAAGCTCATCGAGAGTAAACATG AGGTTACGATCCTGGGAGGACTTAATGAATTTGTAGTGAAGTTTTATGGACCACAAGGAACACCATATGA AGGCGGAGTATGGAAAGTTAGAGTGGACCTACCTGATAAATACCCTTTCAAATCTCCATCTATAGGATTC ATGAATAAAATTTTCCATCCCAACATTGATGAAGCGTCAGGAACTGTGTGTCTAGATGTAATTAATCAAA CTTGGACAGCTCTCTATGATCTTACCAATATATTTGAGTCCTTCCTGCCTCAGTTATTGGCCTATCCTAA CCCCATAGATCCTCTCAATGGTGACGCTGCAGCCATGTACCTCCACCGACCAGAAGAATACAAGCAGAAA ATTAAAGAGTACATCCAGAAATACGCCACGGAGGAGGCGCTGAAAGAACAGGAAGAGGGTACCGGGGACA GCTCATCGGAGAGCTCTATGTCTGACTTTTCCGAAGATGAGGCCCAGGATATGGAGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202516 protein sequence
Red=Cloning site Green=Tags(s) MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGF MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQK IKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003344 |
ORF Size | 549 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_003344.4 |
RefSeq Size | 5174 bp |
RefSeq ORF | 552 bp |
Locus ID | 7328 |
UniProt ID | P62256 |
Cytogenetics | 7q32.2 |
Domains | UBCc |
Protein Pathways | Ubiquitin mediated proteolysis |
MW | 20.7 kDa |
Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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RC202516L1 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
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RC202516L2 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
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RC202516L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
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RC202516L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1, mGFP tagged | 10 ug |
$600.00
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RG202516 | UBE2H (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1 | 10 ug |
$489.00
MSRP
$500.00
MSRP
$500.00
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SC111701 | UBE2H (untagged)-Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1 | 10 ug |
$300.00
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.